Phenotypes associated with this allele

• Allele Symbol: Traf6^tm2Ywc
• Allele Name: targeted mutation 2, Yongwon Choi
• Allele ID: MGI:3773273
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd28^tm1Mak/Cd28^tm1Mak Tg(Cd4-cre)1Cwi/? Traf6^tm2Ywc/Traf6^tm2Ywc	involves: 129S2/SvPas * C57BL/6 * DBA/2	MGI:3773280
cn2	Traf6^tm2Ywc/Traf6^tm2Ywc Tg(Cd4-cre)1Cwi/?	involves: C57BL/6 * DBA/2	MGI:3773278
cn3	Tg(Cd4-cre)1Cwi/? Tg(TcraTcrb)425Cbn/? Traf6^tm2Ywc/Traf6^tm2Ywc	involves: C57BL/6 * DBA/2	MGI:3773279

Genotype
MGI:3773280

cn1

• Allelic Composition: Cd28^tm1Mak/Cd28^tm1Mak; Tg(Cd4-cre)1Cwi/?; Traf6^tm2Ywc/Traf6^tm2Ywc
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:130882 )

• CD4 T cells proliferate in vitro at normal levels when stimulated with anti-CD3 antibody compared

increased memory T cell number ( J:130882 )

• increased number of CD44^highCD62^low T cells in the spleen and lymph nodes

increased activated T cell number ( J:130882 )

• increased number of CD69⁺ T cells are found in the spleen and lymph nodes

decreased interleukin-2 secretion ( J:130882 )

• CD4 T cells secrete IL-2 upon stimulation with anti -CD3 or -CD3/-CD28 antibodies

hematopoietic system

decreased T cell proliferation ( J:130882 )

• CD4 T cells proliferate in vitro at normal levels when stimulated with anti-CD3 antibody compared

increased memory T cell number ( J:130882 )

• increased number of CD44^highCD62^low T cells in the spleen and lymph nodes

increased activated T cell number ( J:130882 )

• increased number of CD69⁺ T cells are found in the spleen and lymph nodes

cellular

decreased T cell proliferation ( J:130882 )

• CD4 T cells proliferate in vitro at normal levels when stimulated with anti-CD3 antibody compared

Genotype
MGI:3773278

cn2

• Allelic Composition: Traf6^tm2Ywc/Traf6^tm2Ywc; Tg(Cd4-cre)1Cwi/?
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

increased memory T cell number ( J:130882 )

• increased number of CD44^highCD62^low T cells are found in the spleen and lymph nodes

increased activated T cell number ( J:130882 )

• increased number of CD69⁺ T cells are found in the spleen and lymph nodes

abnormal lymphocyte anergy ( J:130882 )

• CD4 T cells are resistant to anergy induced in vitro as measured by cellular proliferation in response to anti-CD3 restimulation

• proliferation rates are 5-10 fold higher upon restimulation compared to wild-type controls

• CD4 T cells expressing the Vbeta8 chain continue to proliferate upon restimulation with the superantigen SEB compared to CD4 T cells from control mice that are tolerized and do not proliferate in response to restimulation

hematopoietic system

increased memory T cell number ( J:130882 )

• increased number of CD44^highCD62^low T cells are found in the spleen and lymph nodes

increased activated T cell number ( J:130882 )

• increased number of CD69⁺ T cells are found in the spleen and lymph nodes

Genotype
MGI:3773279

cn3

• Allelic Composition: Tg(Cd4-cre)1Cwi/?; Tg(TcraTcrb)425Cbn/?; Traf6^tm2Ywc/Traf6^tm2Ywc
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

increased interleukin-2 secretion ( J:130882 )

• CD4 T cells from mice immunized with OVA antigen secrete almost 10-fold more IL-2 upon in vitro antigen restimulate compared to mice transgenic only for the T cell receptor

abnormal lymphocyte anergy ( J:130882 )

• OVA antigen specific T cells continue to proliferate upon antigen restimulation in a transfer model of tolerance