Phenotypes associated with this allele

• Allele Symbol: Plcg2^tm1.1Kuro
• Allele Name: targeted mutation 1.1, Tomohiro Kurosaki
• Allele ID: MGI:3773674
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plcg2^tm1.1Kuro/Plcg2^tm1.1Kuro	B6.Cg-Plcg2^tm1.1Kuro	MGI:3777288
hm2	Plcg2^tm1.1Kuro/Plcg2^tm1.1Kuro	Not Specified	MGI:3844212
cx3	Blnk^tm1Kplm/Blnk^tm1Kplm Plcg2^tm1.1Kuro/Plcg2^tm1.1Kuro	involves: 129P2/OlaHsd	MGI:3844213
cx4	Blnk^tm1Kplm/Blnk^tm1Kplm Plcg2^tm1.1Kuro/Plcg2^+	involves: 129P2/OlaHsd	MGI:3844214

Genotype
MGI:3777288

hm1

• Allelic Composition: Plcg2^tm1.1Kuro/Plcg2^tm1.1Kuro
• Genetic Background: B6.Cg-Plcg2^tm1.1Kuro

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

impaired natural killer cell mediated cytotoxicity ( J:131621 )

• unlike wild-type cells, cytokine-activated natural killer cells fail to kill YAC-1 or BaF/3-Rae1gamma cells

decreased interferon-gamma secretion ( J:131621 )

• unlike wild-type cells, NKG2D-stimulated natural killer (NK) cells fail to produce IFN-gamma

• however, NK cells produce large amounts of IFN-gamma when exposed to YAC-1 cells

hematopoietic system

impaired natural killer cell mediated cytotoxicity ( J:131621 )

• unlike wild-type cells, cytokine-activated natural killer cells fail to kill YAC-1 or BaF/3-Rae1gamma cells

Genotype
MGI:3844212

hm2

• Allelic Composition: Plcg2^tm1.1Kuro/Plcg2^tm1.1Kuro
• Genetic Background: Not Specified

immune system

spleen hyperplasia ( J:131161 )

• spleen cellularity is increased by about 50% compared to controls

abnormal B cell differentiation ( J:131161 )

• B cell development is partially blocked at an early transition stage but not to the degree that Blnk null homozygotes are

• evidence includes a lower number of peripheral B cells, an increase percentage of B220⁺ IgL^- cells and a 10-fold increase in the number of marrow cells expressing pre-BCR

increased pro-B cell number ( J:131161 )

• there is an increase in B220⁺ IgL^- cells in the bone marrow

abnormal NK cell physiology ( J:187430 )

• NK cells exhibit partially reduced conjugation with RMA-S cells compared with wild-type cells

• decreased killing of hematopoietic cells RMA-S and YAC-1 cells

hematopoietic system

spleen hyperplasia ( J:131161 )

• spleen cellularity is increased by about 50% compared to controls

abnormal B cell differentiation ( J:131161 )

• B cell development is partially blocked at an early transition stage but not to the degree that Blnk null homozygotes are

• evidence includes a lower number of peripheral B cells, an increase percentage of B220⁺ IgL^- cells and a 10-fold increase in the number of marrow cells expressing pre-BCR

increased pro-B cell number ( J:131161 )

• there is an increase in B220⁺ IgL^- cells in the bone marrow

abnormal NK cell physiology ( J:187430 )

• NK cells exhibit partially reduced conjugation with RMA-S cells compared with wild-type cells

• decreased killing of hematopoietic cells RMA-S and YAC-1 cells

growth/size/body

spleen hyperplasia ( J:131161 )

• spleen cellularity is increased by about 50% compared to controls

Genotype
MGI:3844213

cx3

• Allelic Composition: Blnk^tm1Kplm/Blnk^tm1Kplm; Plcg2^tm1.1Kuro/Plcg2^tm1.1Kuro
• Genetic Background: involves: 129P2/OlaHsd

mortality/aging

perinatal lethality, incomplete penetrance ( J:131161 )

• many mice have perinatal deaths due to a bleeding disorder associated with PLCgamma-2 deficiency

• at weaning, litters from double heterozygote parents only yielded 1 out of 40 pups that were double homozygotes

immune system

increased B cell apoptosis ( J:131161 )

• 40% of B cells are dead after 2 hours in culture with 60% after 4 hours and 80% dead after six hours

decreased B cell number ( J:131161 )

• B cell lineage cells are reduced 3- to 5-fold compared to mice null homozygotes for just one of the alleles

• splenic B cell numbers are reduced to 4.1% of lymphocytes

spleen hypoplasia ( J:131161 )

• spleen cellularity is about one half of that in wild-type mice

hematopoietic system

increased B cell apoptosis ( J:131161 )

• 40% of B cells are dead after 2 hours in culture with 60% after 4 hours and 80% dead after six hours

decreased B cell number ( J:131161 )

• B cell lineage cells are reduced 3- to 5-fold compared to mice null homozygotes for just one of the alleles

• splenic B cell numbers are reduced to 4.1% of lymphocytes

spleen hypoplasia ( J:131161 )

• spleen cellularity is about one half of that in wild-type mice

cellular

increased B cell apoptosis ( J:131161 )

• 40% of B cells are dead after 2 hours in culture with 60% after 4 hours and 80% dead after six hours

Genotype
MGI:3844214

cx4

• Allelic Composition: Blnk^tm1Kplm/Blnk^tm1Kplm; Plcg2^tm1.1Kuro/Plcg2⁺
• Genetic Background: involves: 129P2/OlaHsd

immune system

increased B cell apoptosis ( J:131161 )

• 40% of B cells are dead after 2 hours in culture with 50% after 4 hours and 70% dead after 6 hours

decreased B cell number ( J:131161 )

• splenic B cell numbers are reduced to 13.5% of lymphocytes

abnormal B cell activation ( J:131161 )

• there is a 4.3 fold decrease in the concentration of calcium fluxed intracellularly after stimulus compared to wild-type controls

hematopoietic system

increased B cell apoptosis ( J:131161 )

• 40% of B cells are dead after 2 hours in culture with 50% after 4 hours and 70% dead after 6 hours

decreased B cell number ( J:131161 )

• splenic B cell numbers are reduced to 13.5% of lymphocytes

abnormal B cell activation ( J:131161 )

• there is a 4.3 fold decrease in the concentration of calcium fluxed intracellularly after stimulus compared to wild-type controls

cellular

increased B cell apoptosis ( J:131161 )

• 40% of B cells are dead after 2 hours in culture with 50% after 4 hours and 70% dead after 6 hours