Analysis Tools
mortality/aging
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• lethality between E11 and E12
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growth/size/body
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• smaller by E9.5
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embryo
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• smaller by E9.5
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• the telencephalic neural folds fail to close, giving rise to a disorganized neuroepithelium
• unlike wild-type neuroepithelium at E8.5 which shows long and branched adherens junctions, mutant neuroepithelium shows short and uniformly simple adherens junctions
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• open anterior neural folds at E9.5
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cardiovascular system
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• embryos exhibit engorged cardinal veins
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• actin cables within the sarcomeres of E9.5 cardiomyocytes are disorganized and fail to associate with the fascia adherens
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• lack of ventricular septation
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• marker analysis indicates a markedly underdeveloped, but detectable right ventricle
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• massively dilated pericardial sacs
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• pericardial effusion is seen at E11.5
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nervous system
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• ex vivo, neuronal precursor cell migration across a matrigel-coated filter is reduced by 50% compared to wild-type
|
|
• the telencephalic neural folds fail to close, giving rise to a disorganized neuroepithelium
• unlike wild-type neuroepithelium at E8.5 which shows long and branched adherens junctions, mutant neuroepithelium shows short and uniformly simple adherens junctions
|
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• open anterior neural folds at E9.5
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exencephaly
(
J:131432
)
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• although caudal neural tube closure appears to occur normally, the spinal cord exhibits a reduction in volume
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hematopoietic system
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• yolk sac progenitor cells that are treated with thrombopoietin ex vivo differentiate into megakaryoctyes normally, however they show impaired membrane-cytoskeletal association, extending membrane blebs rather than normal sheet-like lamellipodia when placed on fibrinogen
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homeostasis/metabolism
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• pericardial effusion is seen at E11.5
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cellular
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• ex vivo, neuronal precursor cell migration across a matrigel-coated filter is reduced by 50% compared to wild-type
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