Phenotypes associated with this allele

• Allele Symbol: E2f7^tm1.1Gle
• Allele Name: targeted mutation 1.1, Gustavo Leone
• Allele ID: MGI:3773722
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	E2f7^tm1.1Gle/E2f7^tm1.1Gle	involves: 129S6/SvEvTac * FVB/N	MGI:3797852
cx2	E2f7^tm1.1Gle/E2f7^tm1.1Gle E2f8^tm1.1Gle/E2f8^tm1.1Gle	involves: 129S6/SvEvTac	MGI:5473264
cx3	E2f3^tm2.1Gle/E2f3^tm2.1Gle E2f7^tm1.1Gle/E2f7^tm1.1Gle E2f8^tm1.1Gle/E2f8^tm1.1Gle	involves: 129S6/SvEvTac	MGI:5473265
cx4	E2f7^tm1.1Gle/E2f7^tm1.1Gle E2f8^tm1.1Gle/E2f8^tm1.1Gle	involves: 129S6/SvEvTac * FVB/N	MGI:3797854
cx5	E2f7^tm1.1Gle/E2f7^tm1.1Gle E2f8^tm1.1Gle/E2f8^+	involves: 129S6/SvEvTac * FVB/N	MGI:3797862
cx6	E2f7^tm1.1Gle/E2f7^+ E2f8^tm1.1Gle/E2f8^tm1.1Gle	involves: 129S6/SvEvTac * FVB/N	MGI:3797864

Genotype
MGI:3797852

hm1

• Allelic Composition: E2f7^tm1.1Gle/E2f7^tm1.1Gle
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:131292 )

• pups develop normally through puberty and live to old age

Genotype
MGI:5473264

cx2

• Allelic Composition: E2f7^tm1.1Gle/E2f7^tm1.1Gle; E2f8^tm1.1Gle/E2f8^tm1.1Gle
• Genetic Background: involves: 129S6/SvEvTac

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:183999 )

• no live embryos are recovered after E10.5

• double mutants with wild-type (Sox2-cre E2f7^fl/- E28^fl/-) placentas appear normal at E10.5 and lack the developmental phenotypes in double null embryos

• Note: Sox2-cre is expressed only in the inner cell mass with no extraembronic tissue expression so the placentas are normal

• however, embryos rescued from lethality during development die within a few days of birth

growth/size/body

fetal growth retardation ( J:183999 )

embryo

abnormal placental labyrinth vasculature morphology ( J:183999 )

• vascular network is poorly formed, with maternal sinusoids rarely adjacent to fetal blood vessels

increased placenta apoptosis ( J:183999 )

• placentas have an increased number of apoptotic spongiotrophoblast cells

abnormal embryo development ( J:183999 )

• embryos show profound stress response

• widespread apoptosis is observed in embryos

small placenta ( J:183999 )

• placentas are smaller overall

abnormal trophoblast layer morphology ( J:183999 )

• abnormally large clusters of densely packed trophoblast cells which do not effectively invade the maternal decidua

abnormal spongiotrophoblast cell morphology ( J:183999 )

• placentas have an increased number of apoptotic spongiotrophoblast cells

abnormal placenta development ( J:183999 )

• placentas display altered differentiation of multiple extraembryonic cell lineages

cellular

increased placenta apoptosis ( J:183999 )

• placentas have an increased number of apoptotic spongiotrophoblast cells

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:183999 )

• vascular network is poorly formed, with maternal sinusoids rarely adjacent to fetal blood vessels

Genotype
MGI:5473265

cx3

• Allelic Composition: E2f3^tm2.1Gle/E2f3^tm2.1Gle; E2f7^tm1.1Gle/E2f7^tm1.1Gle; E2f8^tm1.1Gle/E2f8^tm1.1Gle
• Genetic Background: involves: 129S6/SvEvTac

embryo

embryo phenotype ( J:183999 )

N • placentas appear normal with well-organized labyrinthine architecture and vasculature, and are functional as fetuses lack signs of acute stress response or ectopic cell apoptosis

N • live embryos/fetuses are recovered at all stages analyzed, including at birth

Genotype
MGI:3797854

cx4

• Allelic Composition: E2f7^tm1.1Gle/E2f7^tm1.1Gle; E2f8^tm1.1Gle/E2f8^tm1.1Gle
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:131292 )

• by E10.5, only 45% of double mutants are alive and by E11.5 all are dead

growth/size/body

decreased embryo size ( J:131292 )

• smaller at E9.5

embryo

abnormal vitelline vasculature morphology ( J:131292 )

• live E10.5 embryos often have vascular defects in the yolk sac

increased embryonic tissue cell apoptosis ( J:131292 )

• massive apoptosis in embryos at E9.5; multiple areas within the head mesenchyme, branchial arch, somites, and neural tube contain cells with pyknotic nuclei surrounded by bright eosinophilic cytoplasm

decreased embryo size ( J:131292 )

• smaller at E9.5

cardiovascular system

abnormal blood vessel morphology ( J:131292 )

• large dilated blood vessels

abnormal vitelline vasculature morphology ( J:131292 )

• live E10.5 embryos often have vascular defects in the yolk sac

hemorrhage ( J:131292 )

• large dilated blood vessels associated with multifocal hemorrhages

cellular

increased embryonic tissue cell apoptosis ( J:131292 )

• massive apoptosis in embryos at E9.5; multiple areas within the head mesenchyme, branchial arch, somites, and neural tube contain cells with pyknotic nuclei surrounded by bright eosinophilic cytoplasm

Genotype
MGI:3797862

cx5

• Allelic Composition: E2f7^tm1.1Gle/E2f7^tm1.1Gle; E2f8^tm1.1Gle/E2f8⁺
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

mortality/aging

premature death ( J:131292 )

• most die within the first 3 months of life

growth/size/body

decreased body size ( J:131292 )

• most mutants appear runted

Genotype
MGI:3797864

cx6

• Allelic Composition: E2f7^tm1.1Gle/E2f7⁺; E2f8^tm1.1Gle/E2f8^tm1.1Gle
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

normal phenotype

no abnormal phenotype detected ( J:131292 )

• mutants appear developmentally normal