Analysis Tools|
Allele Symbol Allele Name Allele ID |
E2f8tm1.1Gle targeted mutation 1.1, Gustavo Leone MGI:3773724 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no live embryos are recovered after E10.5
• double mutants with wild-type (Sox2-cre E2f7fl/- E28fl/-) placentas appear normal at E10.5 and lack the developmental phenotypes in double null embryos
• Note: Sox2-cre is expressed only in the inner cell mass with no extraembronic tissue expression so the placentas are normal
• however, embryos rescued from lethality during development die within a few days of birth
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• vascular network is poorly formed, with maternal sinusoids rarely adjacent to fetal blood vessels
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• placentas have an increased number of apoptotic spongiotrophoblast cells
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• embryos show profound stress response
• widespread apoptosis is observed in embryos
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• placentas are smaller overall
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• abnormally large clusters of densely packed trophoblast cells which do not effectively invade the maternal decidua
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• placentas have an increased number of apoptotic spongiotrophoblast cells
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• placentas display altered differentiation of multiple extraembryonic cell lineages
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• placentas have an increased number of apoptotic spongiotrophoblast cells
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• vascular network is poorly formed, with maternal sinusoids rarely adjacent to fetal blood vessels
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• placentas appear normal with well-organized labyrinthine architecture and vasculature, and are functional as fetuses lack signs of acute stress response or ectopic cell apoptosis
• live embryos/fetuses are recovered at all stages analyzed, including at birth
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• by E10.5, only 45% of double mutants are alive and by E11.5 all are dead
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• smaller at E9.5
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• live E10.5 embryos often have vascular defects in the yolk sac
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• massive apoptosis in embryos at E9.5; multiple areas within the head mesenchyme, branchial arch, somites, and neural tube contain cells with pyknotic nuclei surrounded by bright eosinophilic cytoplasm
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• smaller at E9.5
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• large dilated blood vessels
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• live E10.5 embryos often have vascular defects in the yolk sac
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• large dilated blood vessels associated with multifocal hemorrhages
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• massive apoptosis in embryos at E9.5; multiple areas within the head mesenchyme, branchial arch, somites, and neural tube contain cells with pyknotic nuclei surrounded by bright eosinophilic cytoplasm
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• most die within the first 3 months of life
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• most mutants appear runted
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mutants appear developmentally normal
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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