Phenotypes associated with this allele

• Allele Symbol: Eomes^tm1Rob
• Allele Name: targeted mutation 1, Elizabeth J Robertson
• Allele ID: MGI:3774027
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Eomes^tm1Rob/Eomes^tm1Rob	involves: 129S/SvEv	MGI:3775731
cn2	Eomes^tm1Rob/Eomes^tm1.1Rob Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^+ Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S/SvEv * 129S4/SvJae * C57BL/6 * CBA	MGI:3775735
cn3	Eomes^tm1Rob/Eomes^tm1.1Rob Tg(T-cre)1Lwd/0	involves: 129S/SvEv * C3H * C57BL/6 * CBA	MGI:3775734
cn4	Eomes^tm1Rob/Eomes^+ Nodal^tm1Rob/Nodal^+ Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S/SvEv * C57BL/6 * CBA	MGI:3775737
cn5	Eomes^tm1Rob/Eomes^tm1Rob Sox1^tm1(cre)Take/0	involves: 129S/SvEv * C57BL/6 * CBA	MGI:3808022
cn6	Eomes^tm1Rob/Eomes^tm2.1Rob Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S/SvEv * C57BL/6 * CBA	MGI:4399139
cn7	Eomes^tm1Rob/Eomes^tm1.1Rob Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S/SvEv * C57BL/6 * CBA	MGI:3775733

Genotype
MGI:3775731

hm1

• Allelic Composition: Eomes^tm1Rob/Eomes^tm1Rob
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:131055 )

• animals are recovered in normal numbers at weaning; mice are fertile and show no overt abnormalities

Genotype
MGI:3775735

cn2

• Allelic Composition: Eomes^tm1Rob/Eomes^tm1.1Rob; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S/SvEv * 129S4/SvJae * C57BL/6 * CBA

embryo

abnormal endoderm development ( J:131055 )

• specification of the definitive endoderm is disrupted

abnormal visceral endoderm morphology ( J:131055 )

• visceral endoderm fails to displace proximally at E7.5

Genotype
MGI:3775734

cn3

• Allelic Composition: Eomes^tm1Rob/Eomes^tm1.1Rob; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S/SvEv * C3H * C57BL/6 * CBA

embryo

embryo phenotype ( J:131055 )

N • Mendelian numbers of conditionally null embryos are recovered around E6.5

Genotype
MGI:3775737

cn4

• Allelic Composition: Eomes^tm1Rob/Eomes⁺; Nodal^tm1Rob/Nodal⁺; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

mortality/aging

prenatal lethality ( J:131055 )

• severely affected embryos are not recovered

embryo

rostral body truncation ( J:131055 )

• embryos with anterior axis truncations are recovered at low frequency

Genotype
MGI:3808022

cn5

• Allelic Composition: Eomes^tm1Rob/Eomes^tm1Rob; Sox1^tm1(cre)Take/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

behavior/neurological

increased exploration in new environment ( J:138764 )

• increased exploratory behavior in the open field test

increased aggression towards humans ( J:138764 )

• enhanced aggressiveness upon handling

increased aggression towards mice ( J:138764 )

• male mutants mutilated or killed pups

decreased grip strength ( J:138764 )

• significantly reduced grip strength

nervous system

abnormal neuron apoptosis ( J:138764 )

• enhanced cell death in the dentate migration stream

abnormal axon guidance ( J:138764 )

• fraction of callosal axons are misrouted

• the typical input-related patterning in Barrel cortex was strongly diminished

abnormal neuronal precursor proliferation ( J:138764 )

• significant decrease of nonventricular surface proliferation during cortical development

• expression of subventricular zone (SVZ) markers were reduced indicating about 25% decrease of proliferating cells in the mutant SVZ throughout development

• neurogenesis in the dentate gyrus was completely absent

• nerurogenesis in th subependymal zone of adult mutants appeared normal

decreased brain size ( J:138764 )

• despite normal body weight, mutant brains showed a markedly reduced brain size

• the size of the cortex and the olfactory bulbs was markedly reduced at birth

absent anterior commissure ( J:138764 )

• the contralateral projecting fibers from olfactory nuclei and temporal lobes which forms the anterior commissure were absent

abnormal dentate gyrus morphology ( J:138764 )

• the infrapyramidal blade did not develop

thin cerebral cortex ( J:138764 )

• cortical layers II-IV thickness is reduced

abnormal olfactory bulb morphology ( J:138764 )

• the mitral cell layer in the olfactory bulb is absent

small olfactory bulb ( J:138764 )

cellular

abnormal neuron apoptosis ( J:138764 )

• enhanced cell death in the dentate migration stream

abnormal axon guidance ( J:138764 )

• fraction of callosal axons are misrouted

• the typical input-related patterning in Barrel cortex was strongly diminished

abnormal neuronal precursor proliferation ( J:138764 )

• significant decrease of nonventricular surface proliferation during cortical development

• expression of subventricular zone (SVZ) markers were reduced indicating about 25% decrease of proliferating cells in the mutant SVZ throughout development

• neurogenesis in the dentate gyrus was completely absent

• nerurogenesis in th subependymal zone of adult mutants appeared normal

Genotype
MGI:4399139

cn6

• Allelic Composition: Eomes^tm1Rob/Eomes^tm2.1Rob; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

Eomes^tm1Rob/Eomes^tm2.1Rob Edil3^{Tg(Sox2-cre)1Amc}/0 embryos fail to form the mesoderm cell layer and are blocked in gastrulation

embryo

failure to gastrulate ( J:154785 )

• mice are blocked in gastrulation

abnormal embryonic epiblast morphology ( J:154785 )

• epiblast cells fails to undergo the epithelial to mesenchyme transition and to form a distinctive mesodermal cell layer unlike in wild-type mice

abnormal primitive streak morphology ( J:154785 )

• unlike in wild-type mice, the primitive streak is populated by nascent mesoderm

Genotype
MGI:3775733

cn7

• Allelic Composition: Eomes^tm1Rob/Eomes^tm1.1Rob; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

embryo

abnormal embryonic epiblast morphology ( J:131055 )

• by late headfold stages, embryos have multiple cystic epiblast invaginations and completely lack a discrete mesoderm layer

absent mesoderm ( J:131055 )

• at E7.5, there is complete absence of mesodermal cells between the visceral endoderm and the epiblast

abnormal primitive streak morphology ( J:131055 )

• mutant embryos display abnormal thickening of the primitive streak at E7.0, resulting in an abnormal embryo shape

• there is accumulation of cells with mesenchymal morphology in the primitive streak