Analysis Tools|
Allele Symbol Allele Name Allele ID |
Eomestm1.1Rob targeted mutation 1.1, Elizabeth J Robertson MGI:3774028 |
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| Summary |
7 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no embryos are recovered at E7.5
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• E7.5 blastocysts after 72 hours in culture either fail to form a a trophoblast outgrowth, or at best, form a rudimentary outgrowth with a few trophoblast giant cells; thus recapitulating the null phenotype
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• specification of the definitive endoderm is disrupted
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• visceral endoderm fails to displace proximally at E7.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• Mendelian numbers of conditionally null embryos are recovered around E6.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• by late headfold stages, embryos have multiple cystic epiblast invaginations and completely lack a discrete mesoderm layer
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• at E7.5, there is complete absence of mesodermal cells between the visceral endoderm and the epiblast
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• mutant embryos display abnormal thickening of the primitive streak at E7.0, resulting in an abnormal embryo shape
• there is accumulation of cells with mesenchymal morphology in the primitive streak
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no phenotypic abnormalities are observed at E9.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• AVE is correctly induced but fails to migrate anteriorly
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• heart patterning abnormalities are observed by E9.5 due to loss of midline structures
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• embryos lack visible germ layers
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• left-right patterning abnormalities are observed by E9.5 due to loss of midline structures
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• floor plate expansion results in increased spacing between somite rows at E9.5
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• by E9.5, mutants lack head structures rostral to the otic placodes
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• in the most severely affected embryos, relatively complete duplications of the posterior body axis are observed, including extra somite rows
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• node abnormalities result in expansion of the floor plate of the neural tube
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• node duplications are accompanied by formation of an accessory notochord
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• at E8.5, development of the node is severely disturbed in a subset of mutants
• complete node duplications are observed in some mutants
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• the most severely affected double heterozygotes show abnormalities around E7 and fail to form a primitive streak
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• addition rows of somites are observed in the most severely affected embryos
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• by late gastrulation stages, embryos are grossly disorganized
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• the most severely affected embryos develop pronounced constrictions between the embryonic and extraembryonic regions of the conceptus
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• embryos frequently show tissue accumulation within the amniotic cavity
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• node abnormalities result in expansion of the floor plate of the neural tube
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• AVE is correctly induced but fails to migrate anteriorly
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• normal Mendelian numbers are recovered during embryogenesis
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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