Phenotypes associated with this allele

• Allele Symbol: Kcnb1^tm1Dgen
• Allele Name: targeted mutation 1, Deltagen
• Allele ID: MGI:3774269
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kcnb1^tm1Dgen/Kcnb1^tm1Dgen	involves: C57BL/6	MGI:3806050
hm2	Kcnb1^tm1Dgen/Kcnb1^tm1Dgen	involves: C57BL/6N	MGI:6383043
cx3	Kcnb1^tm1Dgen/Kcnb1^tm1Dgen Kcnv2^tm1.2(KOMP)Wtsi/Kcnv2^tm1.2(KOMP)Wtsi	involves: 129S6/SvEvTac * C57BL/6N	MGI:6383045

Genotype
MGI:3806050

hm1

• Allelic Composition: Kcnb1^tm1Dgen/Kcnb1^tm1Dgen
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased insulin secretion ( J:129963 )

• isolated islet cells exposed to 7 mM glucose secrete significantly more insulin the wild-type islet cells

• this increased secretion lasts for at least 30 minutes after addition of glucose

hypoglycemia ( J:129963 )

• fasting mice have lower blood glucose levels compared to controls

increased circulating insulin level ( J:129963 )

• levels of insulin during fasting are almost double that of wild-type mice

improved glucose tolerance ( J:129963 )

• fasting mice that are given glucose have an accelerated return to baseline glucose levels

endocrine/exocrine glands

abnormal pancreatic beta cell physiology ( J:129963 )

• beta cells have reduced potassium voltage currents

• at 50mV, the current amplitudes are only 17% that of controls

• action potential duration is increased 3-fold compared to controls during a 5 second duration 2 minutes after depolarization induced by glucose

• the action potential frequency is decreased by over 2-fold

increased insulin secretion ( J:129963 )

• isolated islet cells exposed to 7 mM glucose secrete significantly more insulin the wild-type islet cells

• this increased secretion lasts for at least 30 minutes after addition of glucose

Genotype
MGI:6383043

hm2

• Allelic Composition: Kcnb1^tm1Dgen/Kcnb1^tm1Dgen
• Genetic Background: involves: C57BL/6N

vision/eye

short photoreceptor inner segment ( J:279816 )

short photoreceptor outer segment ( J:279816 )

thin retina outer nuclear layer ( J:279816 )

decreased total retina thickness ( J:279816 )

• due to reduced photoreceptor layers

abnormal cone electrophysiology ( J:279816 )

• reduced flicker amplitude without major delay

abnormal rod electrophysiology ( J:279816 )

• barely discernable a-wave with altered shape, c-wave is completely absent

• reduced amplitudes of oscillatory potentials

nervous system

short photoreceptor inner segment ( J:279816 )

short photoreceptor outer segment ( J:279816 )

Genotype
MGI:6383045

cx3

• Allelic Composition: Kcnb1^tm1Dgen/Kcnb1^tm1Dgen; Kcnv2^{tm1.2(KOMP)Wtsi}/Kcnv2^{tm1.2(KOMP)Wtsi}
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6N

vision/eye

short photoreceptor inner segment ( J:279816 )

short photoreceptor outer segment ( J:279816 )

thin retina outer nuclear layer ( J:279816 )

decreased total retina thickness ( J:279816 )

• due to reduced photoreceptor layers

abnormal cone electrophysiology ( J:279816 )

• reduced flicker amplitude without major delay

abnormal rod electrophysiology ( J:279816 )

• barely discernable a-wave with altered shape, c-wave is completely absent

• altered amplitudes of oscillatory potentials (reduced OP1 and OP2; increased OP3 and OP4)

nervous system

short photoreceptor inner segment ( J:279816 )

short photoreceptor outer segment ( J:279816 )