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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Col6a1-cre)1Gkl
transgene insertion 1, George Kollias
MGI:3775430
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Tnftm2Gkl/Tnf+
Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA MGI:6195612
cn2
 		Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA MGI:6195614
cn3
 		Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/? 		involves: 129S/SvEv * C57BL/6 * CBA MGI:3775437
cn4
 		Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: C57BL/6 * C57BL/6J * CBA MGI:6195560
cn5
 		Map3k8tm1.1Gkl/Map3k8tm1.1Gkl
Tg(Col6a1-cre)1Gkl/0 		involves: C57BL/6 * CBA * FVB/N MGI:5476715


Genotype
MGI:6195612
cn1
Allelic
Composition		 Tnftm2Gkl/Tnf+
Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1btm1.1Gkl mutation (0 available); any Tnfrsf1b mutation (41 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:264147 )
N
• mice show amelioration of the aortic valve stenosis seen in single Tnftm2Gkl heterozygotes, including a decrease of the leaflets surface area and reduction of fibrosis

skeleton
joint inflammation ( J:264147 )
• mice show reduced clinical features of arthritis compared to single Tnftm2Gkl heterozygotes, with a reduction of pannus formation and cartilage/bone destruction

cellular
abnormal cell physiology ( J:264147 )
• valvular interstitial cells fail to become activated
• however, cultured valvular interstitial cells appear similar to wild-type cells and exhibit normal proliferation
abnormal cell adhesion ( J:264147 )
• synovial fibroblast and valvular interstitial cell adhesion to a fibronectin substrate is reduced by almost 50% compared to the levels in single Tnftm2Gkl heterozygotes but is somewhat higher than in wild-type cells
abnormal fibroblast physiology ( J:264147 )
• synovial fibroblasts fail to become activated
• however, cultured synovial fibroblasts appear similar to wild-type cells and exhibit normal proliferation

homeostasis/metabolism
increased circulating tumor necrosis factor level ( J:264147 )
• circulating TNF levels are elevated
abnormal wound healing ( J:264147 )
• synovial fibroblasts and valvular interstitial cells show only 40-45% closure of a wound in culture compared to 70-80% closure in single Tnftm2Gkl heterozygotes and about 20% in wild-type cells

immune system
increased circulating tumor necrosis factor level ( J:264147 )
• circulating TNF levels are elevated
increased tumor necrosis factor secretion ( J:264147 )
• levels of secreted TNF-alpha in supernatants of cultured synovial fibroblasts and valvular interstitial cells are elevated
joint inflammation ( J:264147 )
• mice show reduced clinical features of arthritis compared to single Tnftm2Gkl heterozygotes, with a reduction of pannus formation and cartilage/bone destruction




Genotype
MGI:6195614
cn2
Allelic
Composition		 Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1atm2Gkl mutation (1 available); any Tnfrsf1a mutation (52 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:264147 )
N
• aortic valves appear normal at 16 weeks of age, showing no signs of disease




Genotype
MGI:3775437
cn3
Allelic
Composition		 Tnftm2Gkl/Tnf+
Tnfrsf1atm2Gkl/Tnfrsf1atm2Gkl
Tg(Col6a1-cre)1Gkl/?
Genetic
Background		 involves: 129S/SvEv * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1atm2Gkl mutation (1 available); any Tnfrsf1a mutation (52 available)
Tnftm2Gkl mutation (1 available); any Tnf mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
small intestinal inflammation ( J:131930 )
• the ileum contains inflammatory pathology reminiscent of Crohn's disease
• pathology includes blunting of intestinal villi, and infiltration of inflammation cells into the mucosal and submucosal layers
• 100% of mice have disease by 8 weeks of age

immune system
small intestinal inflammation ( J:131930 )
• the ileum contains inflammatory pathology reminiscent of Crohn's disease
• pathology includes blunting of intestinal villi, and infiltration of inflammation cells into the mucosal and submucosal layers
• 100% of mice have disease by 8 weeks of age
autoimmune arthritis ( J:131930 )
• 100% of mice have arthritis by 8 weeks of age
• joints are infiltrated by mononuclear cells
• mice also develop spondyloarthritis in their sacroiliac joints

skeleton
autoimmune arthritis ( J:131930 )
• 100% of mice have arthritis by 8 weeks of age
• joints are infiltrated by mononuclear cells
• mice also develop spondyloarthritis in their sacroiliac joints




Genotype
MGI:6195560
cn4
Allelic
Composition		 Tnfrsf1btm1.1Gkl/Tnfrsf1btm1.1Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Col6a1-cre)1Gkl mutation (1 available)
Tnfrsf1btm1.1Gkl mutation (0 available); any Tnfrsf1b mutation (41 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:264147 )
• mice are born at the expected ratios, are viable, and fertile and do not display any spontaneous phenotypes




Genotype
MGI:5476715
cn5
Allelic
Composition		 Map3k8tm1.1Gkl/Map3k8tm1.1Gkl
Tg(Col6a1-cre)1Gkl/0
Genetic
Background		 involves: C57BL/6 * CBA * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map3k8tm1.1Gkl mutation (1 available); any Map3k8 mutation (41 available)
Tg(Col6a1-cre)1Gkl mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
decreased enterocyte apoptosis ( J:193546 )
• decreased in mice treated with AOM/DSS
abnormal enterocyte proliferation ( J:193546 )
• increased in mice treated with AOM/DSS
abnormal colon morphology ( J:193546 )
• high-grade dysplasia in mice treated with AOM/DSS

neoplasm
increased incidence of tumors by chemical induction ( J:193546 )
• higher tumor burdens and tumor size in mice treated with AOM/DSS
increased tumor growth/size ( J:193546 )
• higher tumor burdens and tumor size in mice treated with AOM/DSS

cellular
decreased enterocyte apoptosis ( J:193546 )
• decreased in mice treated with AOM/DSS
abnormal enterocyte proliferation ( J:193546 )
• increased in mice treated with AOM/DSS

homeostasis/metabolism
homeostasis/metabolism phenotype ( J:193546 )
N
• mice treated with AOM/DSS exhibit normal body weight loss, decreased colon length, inflammation and tissue damage
increased incidence of tumors by chemical induction ( J:193546 )
• higher tumor burdens and tumor size in mice treated with AOM/DSS




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory