Analysis Tools
growth/size/body
reproductive system
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• homozygous mutant females are infertile unless supplemental iron is administered intraperitoneally or in their diet (2% carbonyl iron)
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hematopoietic system
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• hematologic analysis of homozygous mutant mice reveals decreased mean red blood cell volume and decreased hemoglobin, diagnostic of microcytic anemia
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• mask mutants consuming a standard laboratory diet have low tissue iron stores; far fewer spleen cells of mutant than of wild-type mice stain with Prussion blue
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homeostasis/metabolism
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• despite the anemia of iron-deprived homozygous mutants, their liver levels of mRNA encoding hepcidin, a suppressor of intestinal iron absorption, increase to approximately 8-fold the levels in iron-deprived but non-anemic controls, in whom hepcidin transcription is repressed
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• mask mutants consuming a standard laboratory diet have low tissue iron stores; far fewer spleen cells of mutant than of wild-type mice stain with Prussion blue
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• plasma iron levels of homozygous mutant mice are approximately 3-fold lower thant those of control mice
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immune system
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• mask mutants consuming a standard laboratory diet have low tissue iron stores; far fewer spleen cells of mutant than of wild-type mice stain with Prussion blue
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digestive/alimentary system
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• on a standard diet, intestinal iron absorption by mask mutants is slightly greater than that of non-anemic controls, as measured by 59Fe retention after intragastric administration
• after 4 weeks on an iron-deficient diet, homozygous mutants exhibit only 3.5-fold greater 59Fe absorption efficiency than on a standard diet, compared to a 6-fold increase in absorption efficiency in iron-deprived but non-anemic controls
• despite the anemia of iron-deprived homozygous mutants, their liver levels of mRNA encoding hepcidin, a suppressor of intestinal iron absorption, increase to approximately 8-fold the levels in iron-deprived but non-anemic controls, in whom hepcidin transcription is repressed
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integument
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• mice homozygous for this mutation develop a normal hair coat by postnatal day 10 (P10), but from P13 they gradually lose hair from the trunk, and most are nude or have very sparse body hair by P28; facial hair is unaffected
• feeding or intraperitoneal administration of supplemental iron reverses the hair loss phenotype
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• mice homozygous for this mutation develop a normal hair coat by postnatal day 10 (P10), but from P13 they gradually lose hair from the trunk, and most are nude or have very sparse body hair by P28; facial hair is unaffected
• feeding or intraperitoneal administration of supplemental iron reverses the hair loss phenotype
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