Phenotypes associated with this allele

• Allele Symbol: Egln2^tm1Pec
• Allele Name: targeted mutation 1, Peter Carmeliet
• Allele ID: MGI:3776872
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Egln2^tm1Pec/Egln2^tm1Pec	involves: 129S/SvEv * Swiss	MGI:3777335
cx2	Egln2^tm1Pec/Egln2^tm1Pec Epas1^tm1Pec/Epas1^+	involves: 129S/SvEv * Swiss	MGI:3777336
cx3	Egln2^tm1Pec/Egln2^tm1Pec Hif1a^tm1Pec/Hif1a^+	involves: 129S/SvEv * Swiss	MGI:3777337

Genotype
MGI:3777335

hm1

• Allelic Composition: Egln2^tm1Pec/Egln2^tm1Pec
• Genetic Background: involves: 129S/SvEv * Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

skeletal muscle necrosis ( J:132630 )

• after 7 days of ischemia induced by femoral artery ligation, wild-type mice display extensive muscle necrosis, whereas necrotic area is greatly reduced in mutant muscle

abnormal muscle physiology ( J:132630 )

• respiration in myofibers is reduced compared to wild-type fibers

• oxidation of glucose by muscle is significantly reduced in mutants

• glycolytic flux in muscle under normoxic conditions is increased in mutants

• muscle shows reduced oxidative stress in response to induced ischemia

• under ischemia, myofibers still generate energy whereas wild-type myofibers do not

homeostasis/metabolism

decreased oxygen consumption ( J:132630 )

• whole body oxygen consumption is reduced compared to wild-type; oxygen conservation is also detected during daytime when the mice are inactive

cellular

abnormal mitochondrial physiology ( J:132630 )

• state 3 respiration is severly impaired in wild-type muscle, but only minimally impaired in mutant muscle

• mitochondrial damage is reduced in ischemic muscle compared to wild-type ischemic muscle

Genotype
MGI:3777336

cx2

• Allelic Composition: Egln2^tm1Pec/Egln2^tm1Pec; Epas1^tm1Pec/Epas1⁺
• Genetic Background: involves: 129S/SvEv * Swiss

muscle

muscle phenotype ( J:132630 )

N • Egln2-deficient mice which are heterozygous for Epas1 deficiency show similar muscle necrosis to wild-type following induced ischemia; tolerance to hypoxia conferred by Egln2 loss is abrogated in double mutants

Genotype
MGI:3777337

cx3

• Allelic Composition: Egln2^tm1Pec/Egln2^tm1Pec; Hif1a^tm1Pec/Hif1a⁺
• Genetic Background: involves: 129S/SvEv * Swiss

muscle

muscle phenotype ( J:132630 )

N • double mutants show ischemic muscle damage that is intermediate to Egln2/Epas1 double mutants and Egln2-null mice, so protection is partially lost in these animals