Phenotypes associated with this allele

• Allele Symbol: Wipf3^tm1Tta
• Allele Name: targeted mutation 1, Tadaomi Takenawa
• Allele ID: MGI:3777008
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wipf3^tm1Tta/Wipf3^tm1Tta	involves: 129P2/OlaHsd * C57BL/6J	MGI:3777292

Genotype
MGI:3777292

hm1

• Allelic Composition: Wipf3^tm1Tta/Wipf3^tm1Tta
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

teratozoospermia ( J:132529 )

♂

abnormal sperm head morphology ( J:132529 )

♂ • ~78% of homozygous mutant sperm heads display an abnormal shape compared to sperm from heterozygotes

small sperm head ( J:132529 )

♂ • homozygous mutant sperm heads are extremely thin

abnormal spermatid morphology ( J:132529 )

♂ • at step 16, homozygous mutant spermatids exhibit thinner heads

reproductive system

abnormal spermatogenesis ( J:132529 )

♂ • male homozygotes display defective sperm development at the latest step of spermatogenesis

teratozoospermia ( J:132529 )

♂

abnormal sperm head morphology ( J:132529 )

♂ • ~78% of homozygous mutant sperm heads display an abnormal shape compared to sperm from heterozygotes

small sperm head ( J:132529 )

♂ • homozygous mutant sperm heads are extremely thin

abnormal spermatid morphology ( J:132529 )

♂ • at step 16, homozygous mutant spermatids exhibit thinner heads

decreased litter size ( J:132529 )

• average litter size is 2.5 pups, reduced from litters from heterozygous by wild-type matings

reduced male fertility ( J:132529 )

♂ • when male homozygotes are crossed to wild-type females, only 13% of females become pregnant compared to a rate of 86% of females crossed with heterozygous males

impaired fertilization ( J:132529 )

• in vitro fertilization experiments show reduced ability of mutant sperm to fertilize oocytes; 24 hours after insemination, only 20% of oocytes have become fertilized by sperm from mutant males, compared to around 80% of oocytes when heterozygous sperm is tested

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:132529 )

N • serum testosterone and follicle-stimulating hormone levels are comparable between heterozygous and homozygous animals