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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Nfkbidtm1Clay
targeted mutation 1, Linda K Clayton
MGI:3777661
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Nfkbidtm1Clay/Nfkbidtm1Clay involves: 129S6/SvEvTac * C57BL/6 MGI:3777766
cx2
 		H2b/H2b
Nfkbidtm1Clay/Nfkbidtm1Clay
Rag2tm1Fwa/Rag2tm1Fwa
Tg(TcraN15,TcrbN15)L2Elre/? 		involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6 MGI:3777767


Genotype
MGI:3777766
hm1
Allelic
Composition		 Nfkbidtm1Clay/Nfkbidtm1Clay
Genetic
Background		 involves: 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nfkbidtm1Clay mutation (2 available); any Nfkbid mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:132071 )
• Both CD4- and CD8- T cells proliferate poorly in culture to ConA, anti-CD3 treatment, or anti-CD3 + anti-CD28 treatment
• T cells also have reduced proliferation in response to allogenic MHC
• proliferation defects are intrinsic to T cells as revealed by bone marrow chimeras
• T cells proliferate normally to PMA + ionomycin treatment
abnormal thymocyte activation ( J:132071 )
• thymoyctes proliferate poorly in culture to ConA, anti-CD3 treatment, or anti-CD3 + anti-CD28 treatment
abnormal NK cell physiology ( J:132071 )
• NK cells produce very little IFN-gamma when stimulated in culture
decreased interferon-gamma secretion ( J:132071 )
• thymocytes secrete 2- to 3- fold less IFN-gamma when stimulated in vitro
• CD8 T cells produce very little IFN-gamma when stimulated in vitro CD8 T cells produce very little IFN-gamma when stimulated in vitro
decreased interleukin-2 secretion ( J:132071 )
• thymocytes secrete 2- to 3- fold less IL-2 when stimulated in vitro
• CD8 T cells produce virtually no IL-2 when stimulated in vitro

hematopoietic system
decreased T cell proliferation ( J:132071 )
• Both CD4- and CD8- T cells proliferate poorly in culture to ConA, anti-CD3 treatment, or anti-CD3 + anti-CD28 treatment
• T cells also have reduced proliferation in response to allogenic MHC
• proliferation defects are intrinsic to T cells as revealed by bone marrow chimeras
• T cells proliferate normally to PMA + ionomycin treatment
abnormal thymocyte activation ( J:132071 )
• thymoyctes proliferate poorly in culture to ConA, anti-CD3 treatment, or anti-CD3 + anti-CD28 treatment
abnormal NK cell physiology ( J:132071 )
• NK cells produce very little IFN-gamma when stimulated in culture

endocrine/exocrine glands
abnormal thymocyte activation ( J:132071 )
• thymoyctes proliferate poorly in culture to ConA, anti-CD3 treatment, or anti-CD3 + anti-CD28 treatment

cellular
decreased T cell proliferation ( J:132071 )
• Both CD4- and CD8- T cells proliferate poorly in culture to ConA, anti-CD3 treatment, or anti-CD3 + anti-CD28 treatment
• T cells also have reduced proliferation in response to allogenic MHC
• proliferation defects are intrinsic to T cells as revealed by bone marrow chimeras
• T cells proliferate normally to PMA + ionomycin treatment




Genotype
MGI:3777767
cx2
Allelic
Composition		 H2b/H2b
Nfkbidtm1Clay/Nfkbidtm1Clay
Rag2tm1Fwa/Rag2tm1Fwa
Tg(TcraN15,TcrbN15)L2Elre/?
Genetic
Background		 involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2b mutation (30 available); any H2 mutation (282 available)
Nfkbidtm1Clay mutation (2 available); any Nfkbid mutation (22 available)
Rag2tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(TcraN15,TcrbN15)L2Elre mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:132071 )
• T cells proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen
• T cells require about 2 logs higher concentration of antigen for proliferation equivalent of wild-type T cells
abnormal thymocyte activation ( J:132071 )
• thymocytes proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen
decreased interleukin-2 secretion ( J:132071 )
• lymph node T cells secrete significantly less IL-2 than wild-type T cells in response to irradiated spleen cells loaded with VSV8 antigen

hematopoietic system
decreased T cell proliferation ( J:132071 )
• T cells proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen
• T cells require about 2 logs higher concentration of antigen for proliferation equivalent of wild-type T cells
abnormal thymocyte activation ( J:132071 )
• thymocytes proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

endocrine/exocrine glands
abnormal thymocyte activation ( J:132071 )
• thymocytes proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

cellular
decreased T cell proliferation ( J:132071 )
• T cells proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen
• T cells require about 2 logs higher concentration of antigen for proliferation equivalent of wild-type T cells




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory