Phenotypes associated with this allele

• Allele Symbol: Tg(TcraN15,TcrbN15)L2Elre
• Allele Name: transgene insertion L2, Ellis L Reinherz
• Allele ID: MGI:3777745
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	H2^b/H2^b Nfkbid^tm1Clay/Nfkbid^tm1Clay Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcraN15,TcrbN15)L2Elre/?	involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6	MGI:3777767
cx2	H2^d/H2^d Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcraN15,TcrbN15)L2Elre/?	involves: 129S/SvEv * C57BL/6	MGI:3777752
cx3	H2^b/H2^b Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcraN15,TcrbN15)L2Elre/?	involves: 129S/SvEv * C57BL/6	MGI:3777753

Genotype
MGI:3777767

cx1

• Allelic Composition: H2^b/H2^b; Nfkbid^tm1Clay/Nfkbid^tm1Clay; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraN15,TcrbN15)L2Elre/?
• Genetic Background: involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:132071 )

• T cells proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

• T cells require about 2 logs higher concentration of antigen for proliferation equivalent of wild-type T cells

abnormal thymocyte activation ( J:132071 )

• thymocytes proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

decreased interleukin-2 secretion ( J:132071 )

• lymph node T cells secrete significantly less IL-2 than wild-type T cells in response to irradiated spleen cells loaded with VSV8 antigen

hematopoietic system

decreased T cell proliferation ( J:132071 )

• T cells proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

• T cells require about 2 logs higher concentration of antigen for proliferation equivalent of wild-type T cells

abnormal thymocyte activation ( J:132071 )

• thymocytes proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

endocrine/exocrine glands

abnormal thymocyte activation ( J:132071 )

• thymocytes proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

cellular

decreased T cell proliferation ( J:132071 )

• T cells proliferate poorly to irradiated, wild-type spleen cells loaded with VSV8 antigen

• T cells require about 2 logs higher concentration of antigen for proliferation equivalent of wild-type T cells

Genotype
MGI:3777752

cx2

• Allelic Composition: H2^d/H2^d; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraN15,TcrbN15)L2Elre/?
• Genetic Background: involves: 129S/SvEv * C57BL/6

immune system

arrested T cell differentiation ( J:133112 )

• the majority of T cells in the thymus are arrested at the double positive stage

• single positive T cells are virtually absent

• however, the TCR transgene rescues the arrestment of T cells in the double negative stage that occurs in Rag2 deficient background

decreased CD4-positive, alpha-beta T cell number ( J:133112 )

• mature CD4 T cells are severely reduced in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:133112 )

• mature CD8 T cells are virtually absent in the spleen

hematopoietic system

arrested T cell differentiation ( J:133112 )

• the majority of T cells in the thymus are arrested at the double positive stage

• single positive T cells are virtually absent

• however, the TCR transgene rescues the arrestment of T cells in the double negative stage that occurs in Rag2 deficient background

decreased CD4-positive, alpha-beta T cell number ( J:133112 )

• mature CD4 T cells are severely reduced in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:133112 )

• mature CD8 T cells are virtually absent in the spleen

Genotype
MGI:3777753

cx3

• Allelic Composition: H2^b/H2^b; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraN15,TcrbN15)L2Elre/?
• Genetic Background: involves: 129S/SvEv * C57BL/6

immune system

increased T cell proliferation ( J:133112 )

• CD8 T cells proliferate when presented VSV8 peptide by antigen presenting cells

arrested T cell differentiation ( J:133112 )

• the majority of T cells in the thymus are arrested at the double positive stage

• single positive CD4 positive T cells are absent in the thymus

• there is a reduced percentage of single positive CD8 positive T cells

• however, the TCR transgene rescues the arrestment of T cells in the double negative stage that occurs in Rag2 deficient background

• injection of the VSV8 antigen leads to rapid apoptosis and depletion of double-positive thymocytes with a 3-6 fold drop in total number of thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:133112 )

• there are almost 10-fold less CD4 T cells in the spleen

increased CD8-positive, alpha-beta T cell number ( J:133112 )

• there are about 4-fold more CD8 T cells in the spleen

abnormal cytotoxic T cell physiology ( J:133112 )

• CD8 positive T cells exhibit increased cytotoxicity towards cells loaded with VSV8 peptide

hematopoietic system

increased T cell proliferation ( J:133112 )

• CD8 T cells proliferate when presented VSV8 peptide by antigen presenting cells

arrested T cell differentiation ( J:133112 )

• the majority of T cells in the thymus are arrested at the double positive stage

• single positive CD4 positive T cells are absent in the thymus

• there is a reduced percentage of single positive CD8 positive T cells

• however, the TCR transgene rescues the arrestment of T cells in the double negative stage that occurs in Rag2 deficient background

• injection of the VSV8 antigen leads to rapid apoptosis and depletion of double-positive thymocytes with a 3-6 fold drop in total number of thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:133112 )

• there are almost 10-fold less CD4 T cells in the spleen

increased CD8-positive, alpha-beta T cell number ( J:133112 )

• there are about 4-fold more CD8 T cells in the spleen

abnormal cytotoxic T cell physiology ( J:133112 )

• CD8 positive T cells exhibit increased cytotoxicity towards cells loaded with VSV8 peptide

cellular

increased T cell proliferation ( J:133112 )

• CD8 T cells proliferate when presented VSV8 peptide by antigen presenting cells