Phenotypes associated with this allele

• Allele Symbol: Stim1^tm1Rao
• Allele Name: targeted mutation 1, Anjana Rao
• Allele ID: MGI:3778911
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Stim1^tm1Rao/Stim1^tm1Rao Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:3783457
cn2	Stim1^tm1Rao/Stim1^tm1Rao Stim2^tm1Rao/Stim2^tm1Rao Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2 * SJL	MGI:3783460

Genotype
MGI:3783457

cn1

• Allelic Composition: Stim1^tm1Rao/Stim1^tm1Rao; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased lymphocyte cell number ( J:133262 )

• mice exhibit a milder lymphoproliferation than observed in Stim1^tm1Rao/ Stim1^tm1Rao Stim2^tm1Rao/Stim2^tm1Rao Tg(CD4-cre)1Cwi mice

abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )

• CD4+ T cells exhibit no calcium influx after depletion with thapsigargin or stimulation with anti-CD3 antibodies and ionomycin unlike wild-type cells

• CD4+ T cells fail to produce IL-2 after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies unlike wild-type cells

decreased interleukin-2 secretion ( J:133262 )

• CD4+ T cells fail to produce IL-2 after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies unlike wild-type cells

hematopoietic system

increased lymphocyte cell number ( J:133262 )

• mice exhibit a milder lymphoproliferation than observed in Stim1^tm1Rao/ Stim1^tm1Rao Stim2^tm1Rao/Stim2^tm1Rao Tg(CD4-cre)1Cwi mice

abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )

• CD4+ T cells exhibit no calcium influx after depletion with thapsigargin or stimulation with anti-CD3 antibodies and ionomycin unlike wild-type cells

• CD4+ T cells fail to produce IL-2 after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies unlike wild-type cells

Genotype
MGI:3783460

cn2

• Allelic Composition: Stim1^tm1Rao/Stim1^tm1Rao; Stim2^tm1Rao/Stim2^tm1Rao; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

immune system

abnormal immune system morphology ( J:133262 )

abnormal B cell differentiation ( J:133262 )

• mice produce more IgE+ B cells than wild-type mice

abnormal T cell differentiation ( J:133262 )

• mice produce more memory or effector T cells than wild-type mice

• as determined by bone marrow transplant assays, mice exhibit a cell-intrinsic defect in regulatory T cell development

abnormal granulocyte morphology ( J:133262 )

• mice produce more CD11b+ IL-5R+ eosinophils or basophils than wild-type mice

decreased regulatory T cell number ( J:133262 )

• at 5 to 6 weeks of age, mice exhibit fewer regulatory T cells in the thymus, spleen and lymph nodes compared to wild-type mice due to a cell-intrinsic defect in regulatory T cell development

• while the proportion of regulatory T cells increases with age it remains 10% to 20% of wild-type

increased lymphocyte cell number ( J:133262 )

increased germinal center B cell number ( J:133262 )

increased spleen germinal center number ( J:133262 )

abnormal lymph organ size ( J:133262 )

enlarged spleen ( J:133262 )

• in mice older than 8 weeks

enlarged lymph nodes ( J:133262 )

• in mice older than 8 weeks

abnormal immune system physiology ( J:133262 )

decreased T cell proliferation ( J:133262 )

• T cells undergo reduced proliferation compared to wild-type cells after TCR stimulation

increased IgG1 level ( J:133262 )

abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )

• CD4+ T cells exhibit no calcium influx after depletion with thapsigargin or stimulation with anti-CD3 antibodies and ionomycin unlike wild-type cells

• CD4+ T cells produce almost no IL-2 and very little interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells

decreased interferon-gamma secretion ( J:133262 )

• CD4+ T cells produce very little interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells

decreased interleukin-2 secretion ( J:133262 )

• CD4+ T cells produce almost no IL-2 after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells

blepharitis ( J:133262 )

• in mice older than 8 weeks

dermatitis ( J:133262 )

• in mice older than 8 weeks

vision/eye

blepharitis ( J:133262 )

• in mice older than 8 weeks

hematopoietic system

decreased T cell proliferation ( J:133262 )

• T cells undergo reduced proliferation compared to wild-type cells after TCR stimulation

enlarged spleen ( J:133262 )

• in mice older than 8 weeks

abnormal B cell differentiation ( J:133262 )

• mice produce more IgE+ B cells than wild-type mice

abnormal T cell differentiation ( J:133262 )

• mice produce more memory or effector T cells than wild-type mice

• as determined by bone marrow transplant assays, mice exhibit a cell-intrinsic defect in regulatory T cell development

abnormal granulocyte morphology ( J:133262 )

• mice produce more CD11b+ IL-5R+ eosinophils or basophils than wild-type mice

decreased regulatory T cell number ( J:133262 )

• at 5 to 6 weeks of age, mice exhibit fewer regulatory T cells in the thymus, spleen and lymph nodes compared to wild-type mice due to a cell-intrinsic defect in regulatory T cell development

• while the proportion of regulatory T cells increases with age it remains 10% to 20% of wild-type

increased lymphocyte cell number ( J:133262 )

increased germinal center B cell number ( J:133262 )

increased spleen germinal center number ( J:133262 )

increased IgG1 level ( J:133262 )

abnormal CD4-positive, alpha-beta T cell physiology ( J:133262 )

• CD4+ T cells exhibit no calcium influx after depletion with thapsigargin or stimulation with anti-CD3 antibodies and ionomycin unlike wild-type cells

• CD4+ T cells produce almost no IL-2 and very little interferon-gamma after stimulation with phorbol ester PMA and ionomycin or with anti-CD3 and anti-CD28 antibodies compared to wild-type cells

integument

dermatitis ( J:133262 )

• in mice older than 8 weeks

cellular

decreased T cell proliferation ( J:133262 )

• T cells undergo reduced proliferation compared to wild-type cells after TCR stimulation

growth/size/body

enlarged spleen ( J:133262 )

• in mice older than 8 weeks