Phenotypes associated with this allele

• Allele Symbol: Skap1^Gt713Lex
• Allele Name: gene trap 713, Lexicon Genetics
• Allele ID: MGI:3783261
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Skap1^Gt713Lex/Skap1^Gt713Lex	B6.129S5-Skap1^Gt713Lex	MGI:3809764

Genotype
MGI:3809764

hm1

• Allelic Composition: Skap1^Gt713Lex/Skap1^Gt713Lex
• Genetic Background: B6.129S5-Skap1^Gt713Lex

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:126545 )

N • mice exhibit normal lymphoid development, composition of T cell and B cell subsets, and NK cells

abnormal immune system physiology ( J:126545 )

• fewer cells with localized CD3 are present at the immunological synapse between T cells and antigen presenting cells than in wild-type mice

abnormal T cell physiology ( J:126545 )

• stimulated and unstimulated T cells exhibit decreased contact dwelling time with antigen-presenting cells compared to wild-type cells

• adhesion to fibronectin in unstimulated and anti-CD3 antibody stimulated T cells is reduced compared to in wild-type cells

• adhesion of stimulated T cells to ICAM1 is reduced compared to that of wild-type cells

• however, normal ICAM binding can be restored with high levels of stimulation of anti-CD3 antibodies

• 50% fewer stimulated T cells bind to fibronectin than wild-type cells

decreased T cell proliferation ( J:126545 )

• following stimulation with SEA or anti-CD3 antibodies, T cell proliferation is reduced compared to in wild-type mice

• more stimulated T cells remain in M1 compared to in wild-type mice only during the first round of replication

• however, normal proliferation can be restored with high levels of stimulation of anti-CD3 antibodies

abnormal CD4-positive, alpha-beta T cell physiology ( J:126545 )

• in response to coculture with isolated mature dendritic cells in the presence of the superantigen SEA, fewer and 5- to 10-fold smaller aggregates form compared the response of wild-type cells

decreased interferon-gamma secretion ( J:126545 )

• following stimulation with anti-CD3 antibodies, the number of cells expressing IFN-gamma is reduced 40% to 50% compared to in wild-type mice

decreased interleukin-2 secretion ( J:126545 )

• following stimulation with SEA or anti-CD3 antibodies

hematopoietic system

abnormal T cell physiology ( J:126545 )

• stimulated and unstimulated T cells exhibit decreased contact dwelling time with antigen-presenting cells compared to wild-type cells

• adhesion to fibronectin in unstimulated and anti-CD3 antibody stimulated T cells is reduced compared to in wild-type cells

• adhesion of stimulated T cells to ICAM1 is reduced compared to that of wild-type cells

• however, normal ICAM binding can be restored with high levels of stimulation of anti-CD3 antibodies

• 50% fewer stimulated T cells bind to fibronectin than wild-type cells

decreased T cell proliferation ( J:126545 )

• following stimulation with SEA or anti-CD3 antibodies, T cell proliferation is reduced compared to in wild-type mice

• more stimulated T cells remain in M1 compared to in wild-type mice only during the first round of replication

• however, normal proliferation can be restored with high levels of stimulation of anti-CD3 antibodies

abnormal CD4-positive, alpha-beta T cell physiology ( J:126545 )

• in response to coculture with isolated mature dendritic cells in the presence of the superantigen SEA, fewer and 5- to 10-fold smaller aggregates form compared the response of wild-type cells

cellular

decreased T cell proliferation ( J:126545 )

• following stimulation with SEA or anti-CD3 antibodies, T cell proliferation is reduced compared to in wild-type mice

• more stimulated T cells remain in M1 compared to in wild-type mice only during the first round of replication

• however, normal proliferation can be restored with high levels of stimulation of anti-CD3 antibodies