Phenotypes associated with this allele

• Allele Symbol: Tpp2^tm1.1Gnie
• Allele Name: targeted mutation 1.1, Gabriele Niedermann
• Allele ID: MGI:3783750
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tpp2^tm1.1Gnie/Tpp2^tm1.1Gnie	involves: 129S6/SvEvTac	MGI:5697800
hm2	Tpp2^tm1.1Gnie/Tpp2^tm1.1Gnie	involves: 129S6/SvEvTac * C57BL/6	MGI:3785157

Genotype
MGI:5697800

hm1

• Allelic Composition: Tpp2^tm1.1Gnie/Tpp2^tm1.1Gnie
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

enlarged spleen ( J:221205 )

• lymphadenopathy and/or splenomegaly are inconsistently seen in about 50% of mice

abnormal CD4-positive T cell differentiation ( J:221205 )

• advanced differentiation of CD4+ T cells

abnormal CD8-positive, alpha-beta T cell differentiation ( J:221205 )

• increase in the fraction of CD127- CD8+ T cells in naive mice, however most of these cells show increased Klrg1 expression seen in senescent T cells, indicating enhanced differentiation of these cells

increased B cell number ( J:221205 )

• increase in the fraction of CD11c+CD11b+ B cells

increased memory T cell number ( J:221205 )

• increase in CD44^hi memory CD8+ T cells

• most CD8+ T cells have an effector memory phenotype

decreased leukocyte cell number ( J:221205 )

• older mice develop leukopenia

abnormal lymphocyte physiology ( J:221205 )

• the fraction of cells responding to phorbol 12-myristate 13-acetate/ionomycin stimulation with expression of interferon-gamma is enhanced in CD4+ and CD8+ T cells indicating enhanced effector functions of T cells

immune system

enlarged spleen ( J:221205 )

• lymphadenopathy and/or splenomegaly are inconsistently seen in about 50% of mice

abnormal CD4-positive T cell differentiation ( J:221205 )

• advanced differentiation of CD4+ T cells

abnormal CD8-positive, alpha-beta T cell differentiation ( J:221205 )

• increase in the fraction of CD127- CD8+ T cells in naive mice, however most of these cells show increased Klrg1 expression seen in senescent T cells, indicating enhanced differentiation of these cells

increased B cell number ( J:221205 )

• increase in the fraction of CD11c+CD11b+ B cells

increased memory T cell number ( J:221205 )

• increase in CD44^hi memory CD8+ T cells

• most CD8+ T cells have an effector memory phenotype

decreased leukocyte cell number ( J:221205 )

• older mice develop leukopenia

abnormal lymphocyte physiology ( J:221205 )

• the fraction of cells responding to phorbol 12-myristate 13-acetate/ionomycin stimulation with expression of interferon-gamma is enhanced in CD4+ and CD8+ T cells indicating enhanced effector functions of T cells

enlarged lymph nodes ( J:221205 )

• lymphadenopathy and/or splenomegaly are inconsistently seen in about 50% of mice

increased autoantibody level ( J:221205 )

• 8 of 16 mice have anti-nucleolar or anti-cytoplasmic antibodies

• however, hypergammaglobulinemia is not seen

increased anti-nuclear antigen antibody level ( J:221205 )

• 2 of 16 mice develop antinuclear antibodies

growth/size/body

enlarged spleen ( J:221205 )

• lymphadenopathy and/or splenomegaly are inconsistently seen in about 50% of mice

Genotype
MGI:3785157

hm2

• Allelic Composition: Tpp2^tm1.1Gnie/Tpp2^tm1.1Gnie
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

mortality/aging

premature death ( J:133572 )

• elevated mortality after 1 year of age

premature aging ( J:133572 )

• become aged in appearance

• loss of vigor

growth/size/body

weight loss ( J:133572 )

• reduced body mass after 1 year of age

enlarged spleen ( J:133572 )

• premature development of splenomegaly

immune system

increased T cell apoptosis ( J:133572 )

• of double negative cells

• difference from controls increases with age

• increased apoptosis of activated peripheral CD8+ cells

abnormal cytotoxic T cell physiology ( J:133572 )

• reduced response to lymphocytic choriomeningitis virus

abnormal immune system cell morphology ( J:133572 )

decreased T cell number ( J:133572 )

• strong lymphopenia, more pronounced in blood than lymphoid organs

decreased double-positive T cell number ( J:133572 )

• slightly reduced

decreased CD8-positive, alpha-beta T cell number ( J:133572 )

• reduction of splenic CD8+ cells in young adults is greater than the reduction of splenic CD4+ cells

• peripheral CD8+ cells are reduced to 50% of control numbers by 1 year

• memory CD8+ cells are increased in the spleen and liver

increased T cell number ( J:133572 )

increased double-negative T cell number ( J:133572 )

• slightly increased

• slight increase in proliferation

increased single-positive T cell number ( J:133572 )

• slightly increased

abnormal immune system organ morphology ( J:133572 )

thymus atrophy ( J:133572 )

• normal thymus cellularity to 1 year of age followed by a pronounce thymic involution relative to controls

abnormal spleen morphology ( J:133572 )

enlarged spleen ( J:133572 )

• premature development of splenomegaly

increased spleen red pulp amount ( J:133572 )

• enlarged red pulp

abnormal splenic cell ratio ( J:133572 )

• increased splenic granulocytes and granulocyte progenitors

hematopoietic system

increased T cell apoptosis ( J:133572 )

• of double negative cells

• difference from controls increases with age

• increased apoptosis of activated peripheral CD8+ cells

thymus atrophy ( J:133572 )

• normal thymus cellularity to 1 year of age followed by a pronounce thymic involution relative to controls

extramedullary hematopoiesis ( J:133572 )

• premature occurance

decreased T cell number ( J:133572 )

• strong lymphopenia, more pronounced in blood than lymphoid organs

decreased double-positive T cell number ( J:133572 )

• slightly reduced

decreased CD8-positive, alpha-beta T cell number ( J:133572 )

• reduction of splenic CD8+ cells in young adults is greater than the reduction of splenic CD4+ cells

• peripheral CD8+ cells are reduced to 50% of control numbers by 1 year

• memory CD8+ cells are increased in the spleen and liver

increased T cell number ( J:133572 )

increased double-negative T cell number ( J:133572 )

• slightly increased

• slight increase in proliferation

increased single-positive T cell number ( J:133572 )

• slightly increased

abnormal spleen morphology ( J:133572 )

enlarged spleen ( J:133572 )

• premature development of splenomegaly

increased spleen red pulp amount ( J:133572 )

• enlarged red pulp

abnormal splenic cell ratio ( J:133572 )

• increased splenic granulocytes and granulocyte progenitors

abnormal cytotoxic T cell physiology ( J:133572 )

• reduced response to lymphocytic choriomeningitis virus

cellular

abnormal cell cycle ( J:133572 )

• abbreviated S/G2/M phases of cell cycle in double negative T cells

• marked decrease of G2/M double negative T cells

increased T cell apoptosis ( J:133572 )

• of double negative cells

• difference from controls increases with age

• increased apoptosis of activated peripheral CD8+ cells

early cellular replicative senescence ( J:133572 )

• premature cellular senescence in primary skin fibroblasts

• enlarged flattened appearance of cells

• granular appearance of cells

integument

focal hair loss ( J:133572 )

• large bald areas develop after 1 year of age

disheveled coat ( J:133572 )

• after 1 year of age

endocrine/exocrine glands

thymus atrophy ( J:133572 )

• normal thymus cellularity to 1 year of age followed by a pronounce thymic involution relative to controls