Phenotypes associated with this allele

• Allele Symbol: Tg(GFAP-APOE_i4)#Hol
• Allele Name: transgene insertion, David M Holtzman
• Allele ID: MGI:3784297
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Apoe^tm1Unc/Apoe^tm1Unc Tg(GFAP-APOE_i4)#Hol/0	B6.Cg-Apoe^tm1Unc Tg(GFAP-APOE_i4)#Hol	MGI:3784380
cx2	Apoe^tm1Unc/Apoe^tm1Unc Tg(APPV717F)109Ili/0 Tg(GFAP-APOE_i4)#Hol/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3784383

Genotype
MGI:3784380

cx1

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Tg(GFAP-APOE_i4)#Hol/0
• Genetic Background: B6.Cg-Apoe^tm1Unc Tg(GFAP-APOE_i4)#Hol

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal response to new environment ( J:71062 )

• mice require more time to habituate to a novel environment compared to wild-type mice

abnormal spatial learning ( J:71062 )

• at 14 to 17 months of age, mice did not perform as well as Apoe^tm1Unc homozygotes and Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc mice in a rotating holeboard test

• however, mice perform as well as wild-type mice in a rotating holeboard test

impaired spatial working memory ( J:71062 )

• mice exhibit an impairment in working memory in a radial arm maze test compared to wild-type mice

decreased startle reflex ( J:71062 )

• compared to wild-type mice

abnormal stationary movement ( J:71062 )

• mice exhibit increased numbers of fine movement (not related to ambulation) compared to wild-type mice

abnormal locomotor behavior ( J:71062 )

• mice spend less time than wild-type mice and Apoe^tm1Unc homozygotes in the center of an open field

Genotype
MGI:3784383

cx2

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Tg(APPV717F)109Ili/0; Tg(GFAP-APOE_i4)#Hol/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

nervous system

amyloid beta deposits ( J:127846 , J:133058 )

• by 15 to 18 months, 60% of mice exhibit plaques in the hippocampus (J:127846)

• the amount of amyloid deposited is more than in Tg(APPV717F)109Ili Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc mice but much less than in Tg(APPV717F)109Ili Apoe^tm1Unc/Apoe^tm1Unc mice (J:127846)

• plaques are observed in the outer molecular layer of the dentate gyrus and the stratum oriens and radiatum of the hippocampus (J:127846)

• mice exhibit fibrillar plaques in the outer molecular layer of the dentate gyrus (J:127846)

• 55.6% of mice exposed to traumatic brain injury (TBI) at 9 to 10 months of age exhibit amyloid plaques by 12 to 13 months of age while mice not exposed to TBI do not exhibit plaques until 15 months of age (J:133058)

• mice exhibit increased plaque formation and severity following TBI compared to in similarly treated Tg(APPV717F)109Ili Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc mice (J:133058)

• 44% of mice exhibit thioflavine-S+ amyloid staining (fibrillar amyloid) in the molecular layer unlike in Tg(APPV717F)109Ili Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc and Tg(APPV717F)109Ili Apoe^tm1Unc/Apoe^tm1Unc mice (J:133058)

homeostasis/metabolism

amyloid beta deposits ( J:127846 , J:133058 )

• by 15 to 18 months, 60% of mice exhibit plaques in the hippocampus (J:127846)

• the amount of amyloid deposited is more than in Tg(APPV717F)109Ili Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc mice but much less than in Tg(APPV717F)109Ili Apoe^tm1Unc/Apoe^tm1Unc mice (J:127846)

• plaques are observed in the outer molecular layer of the dentate gyrus and the stratum oriens and radiatum of the hippocampus (J:127846)

• mice exhibit fibrillar plaques in the outer molecular layer of the dentate gyrus (J:127846)

• 55.6% of mice exposed to traumatic brain injury (TBI) at 9 to 10 months of age exhibit amyloid plaques by 12 to 13 months of age while mice not exposed to TBI do not exhibit plaques until 15 months of age (J:133058)

• mice exhibit increased plaque formation and severity following TBI compared to in similarly treated Tg(APPV717F)109Ili Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc mice (J:133058)

• 44% of mice exhibit thioflavine-S+ amyloid staining (fibrillar amyloid) in the molecular layer unlike in Tg(APPV717F)109Ili Tg(GFAP-APOE_i3)37Hol Apoe^tm1Unc/Apoe^tm1Unc and Tg(APPV717F)109Ili Apoe^tm1Unc/Apoe^tm1Unc mice (J:133058)