Phenotypes associated with this allele

• Allele Symbol: Zfp148^Gt(XB878)Byg
• Allele Name: gene trap XB878, BayGenomics
• Allele ID: MGI:3784952
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zfp148^Gt(XB878)Byg/Zfp148^Gt(XB878)Byg	involves: 129P2/OlaHsd	MGI:3807505
hm2	Zfp148^Gt(XB878)Byg/Zfp148^Gt(XB878)Byg	involves: 129P2/OlaHsd * C57BL/6	MGI:5496436
ht3	Zfp148^Gt(XB878)Byg/Zfp148^+	involves: 129P2/OlaHsd	MGI:3807504
cx4	Trp53^tm1Tyj/? Zfp148^Gt(XB878)Byg/Zfp148^Gt(XB878)Byg	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:5496437

Genotype
MGI:3807505

hm1

• Allelic Composition: Zfp148^Gt(XB878)Byg/Zfp148^Gt(XB878)Byg
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:133931 )

• embryos are recovered at normal ratios and are indistinguishable from littermates at E8.5, but all homozygous embryos are dead by E10.5

growth/size/body

embryonic growth retardation ( J:133931 )

• embryos recovered around E10.5 appear significantly growth retarded

embryo

abnormal vitelline vasculature morphology ( J:133931 )

• yolk sac vasculature is disorganized, and some lack visible red blood cells whereas some contain only small pools of primitive erythrocytes around E9.5-10.5

embryonic growth retardation ( J:133931 )

• embryos recovered around E10.5 appear significantly growth retarded

open neural tube ( J:133931 )

• embryos recovered around E10.5 display open neural tubes

nervous system

open neural tube ( J:133931 )

• embryos recovered around E10.5 display open neural tubes

cardiovascular system

abnormal vitelline vasculature morphology ( J:133931 )

• yolk sac vasculature is disorganized, and some lack visible red blood cells whereas some contain only small pools of primitive erythrocytes around E9.5-10.5

integument

pallor ( J:133931 )

• embryos recovered around E10.5 are markedly pale

Genotype
MGI:5496436

hm2

• Allelic Composition: Zfp148^Gt(XB878)Byg/Zfp148^Gt(XB878)Byg
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

growth/size/body

postnatal growth retardation ( J:198319 )

• surviving homozygotes show impaired growth

slow postnatal weight gain ( J:198319 )

• surviving male and female mice show reduced body weights and slower weight gain from 5 weeks through adulthood

homeostasis/metabolism

cyanosis ( J:198319 )

• cyanotic at P1, suggesting respiratory difficulties

integument

pallor ( J:198319 )

• pups are pale at P1

mortality/aging

premature death ( J:198319 )

• homozygotes surviving past 5 weeks have reduced lifespans, with greater than 50% mortality occurring between 20 and 50 weeks

neonatal lethality, incomplete penetrance ( J:198319 )

• homozygotes are found at normal ratios throughout development, but frequency drops to 50% at postnatal day 1

• addition of the antioxidant n-acetyl-L-cysteine (NAC) to maternal drinking water to reduce oxidative stress on embryos prevents the observed neonatal mortality

respiratory system

abnormal lung development ( J:198319 )

• Clara cell markers are reduced in mutant lungs, suggesting that Clara cell differentiation could be affected

• cell proliferation is disrupted (reduced) at the saccular stage of lung development

• addition of the antioxidant n-acetyl-L-cysteine (NAC) to maternal drinking water to reduce oxidative stress on embryos normalizes lung morphology and prevents glycogen accumulation and ectopic CC10 expression

abnormal lung saccule morphology ( J:198319 )

• saccules are poorly developed with thickened primary septae, increased glycogen deposits and widespread ectopic Clara cell marker CC10 expression at E19.5-P1; glycogen deposition and CC10 expression occur prior to birth

Genotype
MGI:3807504

ht3

• Allelic Composition: Zfp148^Gt(XB878)Byg/Zfp148⁺
• Genetic Background: involves: 129P2/OlaHsd

normal phenotype

no abnormal phenotype detected ( J:133931 )

• heterozygotes are indistinguishable from heterozygous or wild-type littermates

Genotype
MGI:5496437

cx4

• Allelic Composition: Trp53^tm1Tyj/?; Zfp148^Gt(XB878)Byg/Zfp148^Gt(XB878)Byg
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

mortality/aging

mortality/aging ( J:198319 )

N • deletion of 1 copy of Trp53 rescues Zfp148^Gt(XB878)Byg homozygotes from neonatal lethality

respiratory system

respiratory system phenotype ( J:198319 )

N • deletion of 1 or 2 copies of Trp53 rescues Zfp148^Gt(XB878)Byg homozygotes from proliferation arrest and lung defects such defective saccule development