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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Npy-MAPT/Sapphire)1Rck
transgene insertion 1, Jeffrey M Friedman
MGI:3785480
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
Lepob/Lepob
Tg(Npy-MAPT/Sapphire)1Rck/0
involves: C57BL/6 * CBA MGI:3785526
cx2
Tg(Npy-MAPT/Sapphire)1Rck/?
Ucp2Gt(S20-3G1)Sor/Ucp2Gt(S20-3G1)Sor
involves: C57BL/6 * CBA MGI:3811500
tg3
Tg(Npy-MAPT/Sapphire)1Rck/0 involves: C57BL/6 * CBA MGI:3785525


Genotype
MGI:3785526
cx1
Allelic
Composition
Lepob/Lepob
Tg(Npy-MAPT/Sapphire)1Rck/0
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lepob mutation (5 available); any Lep mutation (21 available)
Tg(Npy-MAPT/Sapphire)1Rck mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• treatment with 25 ug/g of leptin results in significant decrease in body weight in Lepob homozygotes compared saline-treated mice

nervous system
N
• after 4 days of twice-daily injections of 10 ug of ghrelin, no differences in excitatory or inhibitory synapse number are detected relative to saline-treated mice
• Lepob homozygotes have significantly more synapses onto perikarya of Arcuate nucleus Npy neurons compared to wild-type littermates; in wild-type, inhibitory synapses onto Npy neurons are more numerous than excitatory synapses, whereas in Lepob homozygotes, excitatory synapses onto Npy neurons are more numerous
• altered synaptic profile is consistent with increased excitatory tone onto Npy neurons in Lepob homozygotes
• 6 hours after leptin injection, a reduction in total number of synapses on Npy perikarya is detected, with a decrease in excitatory inputs and increased inhibitory inputs to Npy cells; after 2 days of leptin treatment, synapse number onto Npy neurons is significantly decreased with a 6-fold reduction decrease in excitatory synapse number and a 2-fold increase in inhibitory synapse number in Lepob homozygotes
• similar changes in synaptic profile are observed after 12 days of treatment
• a significantly higher number of spontaneous EPSCs (sEPSCs) onto Npy neurons in hypothalamic slices from Lepob homozygotes is observed compared to Npy neurons in wild-type mice; this is accompanied by a reduction in frequency of sIPSCs relative to wild-type controls
• a tendency toward a decrease in spontaneous EPSC number onto Npy neurons is observed 2 days of leptin treatment in Lepob homozygotes

behavior/neurological
• treatment with 25 ug/g of leptin results in significant decrease in food intake after 2 and 12 days treatment
• after 4 days of twice-daily injections of 10 ug of ghrelin, an increase in food intake is observed




Genotype
MGI:3811500
cx2
Allelic
Composition
Tg(Npy-MAPT/Sapphire)1Rck/?
Ucp2Gt(S20-3G1)Sor/Ucp2Gt(S20-3G1)Sor
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Npy-MAPT/Sapphire)1Rck mutation (1 available)
Ucp2Gt(S20-3G1)Sor mutation (0 available); any Ucp2 mutation (41 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• ghrelin increases mean action potential frequency of GFP-labeled neuronal perikarya in whole-cell recordings of hypothalamic coronal slices from wild-type mice but not in these mice




Genotype
MGI:3785525
tg3
Allelic
Composition
Tg(Npy-MAPT/Sapphire)1Rck/0
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Npy-MAPT/Sapphire)1Rck mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice display no obvious phenotypic abnormalities





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory