Analysis Tools
growth/size/body
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• treatment with 25 ug/g of leptin results in significant decrease in body weight in Lepob homozygotes compared saline-treated mice
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nervous system
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• after 4 days of twice-daily injections of 10 ug of ghrelin, no differences in excitatory or inhibitory synapse number are detected relative to saline-treated mice
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• Lepob homozygotes have significantly more synapses onto perikarya of Arcuate nucleus Npy neurons compared to wild-type littermates; in wild-type, inhibitory synapses onto Npy neurons are more numerous than excitatory synapses, whereas in Lepob homozygotes, excitatory synapses onto Npy neurons are more numerous
• altered synaptic profile is consistent with increased excitatory tone onto Npy neurons in Lepob homozygotes
• 6 hours after leptin injection, a reduction in total number of synapses on Npy perikarya is detected, with a decrease in excitatory inputs and increased inhibitory inputs to Npy cells; after 2 days of leptin treatment, synapse number onto Npy neurons is significantly decreased with a 6-fold reduction decrease in excitatory synapse number and a 2-fold increase in inhibitory synapse number in Lepob homozygotes
• similar changes in synaptic profile are observed after 12 days of treatment
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• a significantly higher number of spontaneous EPSCs (sEPSCs) onto Npy neurons in hypothalamic slices from Lepob homozygotes is observed compared to Npy neurons in wild-type mice; this is accompanied by a reduction in frequency of sIPSCs relative to wild-type controls
• a tendency toward a decrease in spontaneous EPSC number onto Npy neurons is observed 2 days of leptin treatment in Lepob homozygotes
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behavior/neurological
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• treatment with 25 ug/g of leptin results in significant decrease in food intake after 2 and 12 days treatment
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• after 4 days of twice-daily injections of 10 ug of ghrelin, an increase in food intake is observed
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