Phenotypes associated with this allele

• Allele Symbol: Gpx4^tm1Ssd
• Allele Name: targeted mutation 1, Samir S Deeb
• Allele ID: MGI:3785543
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gpx4^tm1Ssd/Gpx4^tm1Ssd	B6.129-Gpx4^tm1Ssd	MGI:3796140
ht2	Gpx4^tm1Ssd/Gpx4^+	B6.129-Gpx4^tm1Ssd	MGI:3796141

Genotype
MGI:3796140

hm1

• Allelic Composition: Gpx4^tm1Ssd/Gpx4^tm1Ssd
• Genetic Background: B6.129-Gpx4^tm1Ssd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:134214 )

• do not survive development past E10.5

Genotype
MGI:3796141

ht2

• Allelic Composition: Gpx4^tm1Ssd/Gpx4⁺
• Genetic Background: B6.129-Gpx4^tm1Ssd

cellular

abnormal mitochondrial physiology ( J:134214 )

• lung fibroblasts have a lower mitochondrial membrane potential, lower levels of reduced thiols, and lower overall levels of cardiolipins

• thiol and cardiolipin oxidation that results from chemical agents causing oxidative stress is reduced in mitochondria of lung fibroblasts

oxidative stress ( J:134214 )

• fibroblasts from lung have increased cytotoxicity to chemicals that cause oxidative stress including low doses of hydrogen peroxide (25 micromolar) and cadmium (concentrations between 75 and 200 micromolar)

• however, fibroblasts have decreased cytotoxicity to phosphatidylcholine hydroperoxide