Phenotypes associated with this allele

• Allele Symbol: Tg(Pmch-ATXN3*)3Rck
• Allele Name: transgene insertion 3, Jeffrey M Friedman
• Allele ID: MGI:3785576
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Lep^ob/Lep^ob Tg(Pmch-ATXN3*)3Rck/0	involves: C57BL/6J * FVB/NJ	MGI:3785732
tg2	Tg(Pmch-ATXN3*)3Rck/0	involves: FVB/NJ	MGI:3785731

Genotype
MGI:3785732

cx1

• Allelic Composition: Lep^ob/Lep^ob; Tg(Pmch-ATXN3*)3Rck/0
• Genetic Background: involves: C57BL/6J * FVB/NJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:104264 )

• at 24-28 weeks, weight is significantly reduced compared to Lep^ob homozygotes

homeostasis/metabolism

decreased circulating glucose level ( J:104264 )

• plasma glucose concentration is significantly reduced compared to Lep^ob homozygotes

behavior/neurological

abnormal maternal nurturing ( J:104264 )

♀ • females can not nurture pups through weaning age; no data is provided

reproductive system

abnormal fertility/fecundity ( J:104264 )

• improvement in fertility is observed; normal-sized litters are produced; no data is provided

liver/biliary system

liver/biliary system phenotype ( J:104264 )

N • steatosis observed in Lep^ob homozygotes is normalized in double mutants

Genotype
MGI:3785731

tg2

• Allelic Composition: Tg(Pmch-ATXN3*)3Rck/0
• Genetic Background: involves: FVB/NJ

growth/size/body

decreased lean body mass ( J:104264 )

• percentage of lean body mass is significantly lower compared to wild-type (at 13 weeks in males, and at 18 weeks in females)

decreased body weight ( J:104264 )

• at 7 weeks of age, male and female transgenic mice show significantly reduced body weights compared to controls; by 22 weeks, reduction is 14% in males and 13% in females

decreased body length ( J:104264 )

adipose tissue

decreased total body fat amount ( J:104264 )

• mice have much less fat mass (40% of levels seen in controls)

decreased percent body fat/body weight ( J:104264 )

♀ • reduced by about half in females at 18 weeks

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:104264 )

N • corticosterone, growth hormone, and insulin-like growth factor levels show no significant differences compared to wild-type (at 13 weeks)

N • hypoleptinemia in mutants causes no changes in mRNA expression for neuropeptide (AGRP, POMC, NPY) in hypothalamus relative to wild-type

N • responses to fasting, leptin treatment and deficiency are normal

decreased circulating glucose level ( J:104264 )

♂ • iat 28 weeks, plasma glucose levels are decreased by 20% in males

decreased circulating insulin level ( J:104264 )

♀ • at 19 weeks, females show a 20% reduction in insulin levels

decreased circulating leptin level ( J:104264 )

♀ • plasma leptin levels are decreased by 30% at 19 weeks in females, but no difference is seen at 28 weeks

increased energy expenditure ( J:104264 )

♂ • males expend significantly more energy and show higher carbon dioxide production than wild-type males

behavior/neurological

decreased food intake ( J:104264 )

• at 16 weeks, females consume 13% less food in 24 hours than wild-type littermates; males also consume less than littermates at 16 weeks of age

• no difference is seen if consumption is corrected for body weight

nervous system

abnormal hypothalamus morphology ( J:104264 )

• ablation of Pmch neurons is detected by postnatal week 4; by postnatal week 8, mice have lost 60% of Pmch expressing neurons in the hypothalamus and by postnatal week 15, 65% of these neurons are lost