All Phenotypes Foxp3<tm4(YFP/icre)Ayr> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Foxp3^{tm4(YFP/icre)Ayr}
targeted mutation 4, Alexander Y Rudensky
MGI:3790499

Summary

28 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Socs1^tm1Ayos/Socs1^tm1Ayos Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:4887825
cn2	Foxo1^tm1Flv/Foxo1^tm1Flv Ifng^tm1Ts/Ifng^tm1Ts Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ	MGI:5448156
cn3	B9d2/Tgfb1^tm1Flv/B9d2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:4943575
cn4	Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:5448157
cn5	B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:4943576
cn6	Foxo1^tm1Flv/Foxo1^tm1Flv Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6	MGI:5448151
cn7	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺ Nr4a2^tm1.1Pcn/Nr4a2^tm1.1Pcn	involves: 129S1/Sv * 129X1/SvJ	MGI:5547376
cn8	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr} Ikzf4^tm1Djr/Ikzf4^tm1Djr	involves: 129S1/Sv * 129X1/SvJ	MGI:6467272
cn9	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}	involves: 129S1/Sv * 129X1/SvJ	MGI:3806256
cn10	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ	MGI:3806255
cn11	Nr4a2^tm1.1Pcn/Nr4a2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ	MGI:5547375
cn12	Il10^tm1Roer/Il10^tm1Roer Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3790650
cn13	Itch^tm1.1Alta/Itch^tm1.1Alta Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5563103
cn14	Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5448149
cn15	Itch^tm1.1Alta/Itch^tm1.1Alta Gt(ROSA)26Sor^tm1Hjf/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5563102
cn16	Becn1^tm1Smoc/Becn1^tm1Smoc Zfp91^tm1Smoc/Zfp91^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:7645512
cn17	Foxp3^{tm4(YFP/icre)Ayr}/Y Zfp91^tm1Smoc/Zfp91^tm1Smoc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:7645491
cn18	Becn1^tm1Smoc/Becn1^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:7645511
cn19	Cd28^tm1Ltu/Cd28^tm1Ltu Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5485238
cn20	Nr4a1^tm1Pcn/Nr4a1^tm1Pcn Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5546602
cn21	Myd88^tm1.1Medz/Myd88^tm1.1Medz Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5616083
cn22	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806254
cn23	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806253
cn24	Altre^tm1.1Hbgl/Altre^tm1.1Hbgl Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:7562295
cn25	Pgd^tm1.1Pse/Pgd^tm1.1Pse Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:7657738
cn26	Areg^{tm2c(EUCOMM)Hmgu}/Areg^{tm2c(EUCOMM)Hmgu} Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:5806476
cn27	Bcl2l11^tm6.1Boui/Bcl2l11^tm6.1Boui Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5562712
cn28	Cish^tm1.1Cdon/Cish^tm1.1Cdon Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S/Sv * 129X1/SvJ * C57BL/6	MGI:5544440

Allelic Composition	Socs1^tm1Ayos/Socs1^tm1Ayos Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Socs1^tm1Ayos mutation (1 available); any Socs1 mutation (30 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased regulatory T cell number ( J:167922 )

blepharitis ( J:167922 )

• by 6 weeks of age

conjunctivitis ( J:167922 )

• by 6 weeks of age

dermatitis ( J:167922 )

• by 6 weeks of age

integument

dermatitis ( J:167922 )

• by 6 weeks of age

vision/eye

blepharitis ( J:167922 )

• by 6 weeks of age

conjunctivitis ( J:167922 )

• by 6 weeks of age

hematopoietic system

increased regulatory T cell number ( J:167922 )

Allelic Composition	Foxo1^tm1Flv/Foxo1^tm1Flv Ifng^tm1Ts/Ifng^tm1Ts Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1^tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Ifng^tm1Ts mutation (18 available); any Ifng mutation (49 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:189962 )

N	• the lethal inflammatory phenotype is partially rescued

immune system

immune system phenotype ( J:189962 )

N	• regulatory T cells are able to suppress development of colitis in Rag1 null mice receiving naive T cells

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

Allelic Composition	B9d2/Tgfb1^tm1Flv/B9d2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B9d2/Tgfb1^tm1Flv mutation (0 available); any B9d2 mutation (13 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice • mice exhibit normal susceptibility to experimental autoimmune encephalomyelitis and Th17 cell differentiation

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

growth/size/body

growth/size/body region phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice

hematopoietic system

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

Allelic Composition	Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:189962 )

• moribund within 35 days of birth

immune system

increased T cell proliferation ( J:189962 )

enlarged spleen ( J:189962 )

increased T cell number ( J:189962 )

• in the spleen and peripheral lymph node

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased regulatory T cell number ( J:189962 )

• in peripheral lymph node at day 20

• however, thymic and splenic regulatory T cell numbers are normal at day 12

abnormal regulatory T cell physiology ( J:189962 )

• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells

enlarged lymph nodes ( J:189962 )

increased interferon-gamma secretion ( J:189962 )

abnormal inflammatory response ( J:189962 )

• infiltrate of leukocytes in the salivary glands, lung interstitia, liver sinusoids, pancreas acini, stomach and colon mucosa

behavior/neurological

hunched posture ( J:189962 )

• lack of mobility associated with hunched posture

paralysis ( J:189962 )

• lack of mobility associated with hunched posture

integument

abnormal skin appearance ( J:189962 )

• crusting of the ears, eyelids and tail

limbs/digits/tail

abnormal tail morphology ( J:189962 )

• scurfy tail

hematopoietic system

increased T cell proliferation ( J:189962 )

enlarged spleen ( J:189962 )

increased T cell number ( J:189962 )

• in the spleen and peripheral lymph node

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased regulatory T cell number ( J:189962 )

• in peripheral lymph node at day 20

• however, thymic and splenic regulatory T cell numbers are normal at day 12

abnormal regulatory T cell physiology ( J:189962 )

• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells

growth/size/body

enlarged spleen ( J:189962 )

cellular

increased T cell proliferation ( J:189962 )

Allelic Composition	B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B9d2/Tgfb1^tm1Flv mutation (0 available); any Tgfb1 mutation (35 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Tgfb1^tm2Flv mutation (0 available); any Tgfb1 mutation (35 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

growth/size/body region phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice

hematopoietic system

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

immune system

immune system phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice • mice exhibit normal susceptibility to experimental autoimmune encephalomyelitis and Th17 cell differentiation

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

Allelic Composition	Foxo1^tm1Flv/Foxo1^tm1Flv Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1^tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli} mutation (1 available); any Gt(ROSA)26Sor mutation (994 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:189962 )

N	• the lethal inflammatory phenotype is completely rescued

immune system

immune system phenotype ( J:189962 )

N	• the lethal inflammatory phenotype is completely rescued

Allelic Composition	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺ Nr4a2^tm1.1Pcn/Nr4a2^tm1.1Pcn
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Nr4a2^tm1.1Pcn mutation (0 available); any Nr4a2 mutation (44 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal regulatory T cell morphology ( J:205658 )

• regulatory T cells are not properly maintained

abnormal regulatory T cell physiology ( J:205658 )

• slightly attenuated suppressive activity

• in a transfer model of colitis, regulatory T cells fail to inhibit wasting disease and colitis compared with wild-type cells

hematopoietic system

abnormal regulatory T cell morphology ( J:205658 )

• regulatory T cells are not properly maintained

abnormal regulatory T cell physiology ( J:205658 )

• slightly attenuated suppressive activity

• in a transfer model of colitis, regulatory T cells fail to inhibit wasting disease and colitis compared with wild-type cells

Allelic Composition	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr} Ikzf4^tm1Djr/Ikzf4^tm1Djr
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Ikzf4^tm1Djr mutation (0 available); any Ikzf4 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

small intestinal inflammation ( J:294549 )

• increased lymphocyte infiltration and neutrophils in lamina propria at age 3 months

enlarged spleen ( J:294549 )

• slight splenomegaly at age 2 months, marked splenomegaly at age 3 months

increased germinal center B cell number ( J:294549 )

• increased percentage in immunized and unimmunized mice at age 3 months

decreased regulatory T cell number ( J:294549 )

• reduced percentage of regulatory follicular T cells in immunized and unimmunized mice at age 3 months

increased T cell number ( J:294549 )

• increased absolute total CD4+, CD8+ and regulatory T cell numbers at age 3 months

• elevated percentage of regulatory T cells at age 3 months

• elevated percentage of CD44hi-CD62Llo CD4+, CD8+ and regulatory T cells at age 3 months

• increased percentage of follicular T cells in unimmunized mice at age 3 months

increased T follicular helper cell number ( J:294549 )

• increased percentage in unimmunized mice at age 3 months

increased spleen B cell follicle number ( J:294549 )

• increased number in immunized mice at age 3 months

increased spleen germinal center number ( J:294549 )

• increased number in immunized mice at age 3 months

increased immunoglobulin level ( J:294549 )

• increased serum levels of all immunoglobulin isotypes and anti-nuclear antibodies (ANA) at age 3 months

enlarged lymph nodes ( J:294549 )

• slight lymphadenopathy at age 2 months, marked lymphadenopathy at age 3 months

increased susceptibility to experimental autoimmune encephalomyelitis ( J:294549 )

• at age 3 months

chronic inflammation ( J:294549 )

• increased lymphocyte infiltration in small intestine, lungs, liver, salivary glands and kidneys at age 3 months

granulomatous inflammation ( J:294549 )

• hepatic microgranulomas at age 3 months

glomerulonephritis ( J:294549 )

• at age 3 months

hematopoietic system

enlarged spleen ( J:294549 )

• slight splenomegaly at age 2 months, marked splenomegaly at age 3 months

increased germinal center B cell number ( J:294549 )

• increased percentage in immunized and unimmunized mice at age 3 months

decreased regulatory T cell number ( J:294549 )

• reduced percentage of regulatory follicular T cells in immunized and unimmunized mice at age 3 months

increased T cell number ( J:294549 )

• increased absolute total CD4+, CD8+ and regulatory T cell numbers at age 3 months

• elevated percentage of regulatory T cells at age 3 months

• elevated percentage of CD44hi-CD62Llo CD4+, CD8+ and regulatory T cells at age 3 months

• increased percentage of follicular T cells in unimmunized mice at age 3 months

increased T follicular helper cell number ( J:294549 )

• increased percentage in unimmunized mice at age 3 months

increased spleen B cell follicle number ( J:294549 )

• increased number in immunized mice at age 3 months

increased spleen germinal center number ( J:294549 )

• increased number in immunized mice at age 3 months

increased immunoglobulin level ( J:294549 )

• increased serum levels of all immunoglobulin isotypes and anti-nuclear antibodies (ANA) at age 3 months

growth/size/body

enlarged spleen ( J:294549 )

• slight splenomegaly at age 2 months, marked splenomegaly at age 3 months

digestive/alimentary system

abnormal small intestine morphology ( J:294549 )

• hyperplastic epithelium at age 3 months

decreased small intestinal microvillus size ( J:294549 )

• blunted at age 3 months

small intestinal inflammation ( J:294549 )

• increased lymphocyte infiltration and neutrophils in lamina propria at age 3 months

renal/urinary system

glomerulonephritis ( J:294549 )

• at age 3 months

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
encephalomyelitis		DOID:640		J:294549

Allelic Composition	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1^tm1Bdh mutation (4 available); any Dicer1 mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

enlarged lymph nodes ( J:138683 )

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

Allelic Composition	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

enlarged lymph nodes ( J:138683 )

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

Allelic Composition	Nr4a2^tm1.1Pcn/Nr4a2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal regulatory T cell morphology ( J:205658 )

• Treg cells are not properly maintained

hematopoietic system

abnormal regulatory T cell morphology ( J:205658 )

• Treg cells are not properly maintained

Allelic Composition	Il10^tm1Roer/Il10^tm1Roer Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Il10^tm1Roer mutation (1 available); any Il10 mutation (45 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

colitis ( J:134513 )

• starting at 10 weeks of age, mice present with the gross manifestations of colitis including colonic thickening and substantial increase in the size of mesenteric lymph nodes

• histological examinations reveals prominent mononuclear infiltration of epithelial tissues as well as tissue destruction of the cecum and portions of the colon

• disease is similar to what is observed in Il10^tm1Cgn null mice though with less severity and incidence, and a slightly later onset

immune system

colitis ( J:134513 )

• starting at 10 weeks of age, mice present with the gross manifestations of colitis including colonic thickening and substantial increase in the size of mesenteric lymph nodes

• histological examinations reveals prominent mononuclear infiltration of epithelial tissues as well as tissue destruction of the cecum and portions of the colon

• disease is similar to what is observed in Il10^tm1Cgn null mice though with less severity and incidence, and a slightly later onset

abnormal regulatory T cell physiology ( J:134513 )

• Foxp3 expressing regulatory CD4 T cells fail to produce IL-10

• mice exhibit spontaneous colitis and inflammation in the lung that is contributable to defective regulatory T cell responses

• mice also have heightened inflammatory responses when challenged with antigen to the lungs or skin

abnormal type IV hypersensitivity reaction ( J:134513 )

• there is a substantial increase in ear thickness and inflammation after application of dinitrofluorobenzene (DNFB) on the ear

• T cells infiltrating the site of DNFB application have higher expression of the activation marker CD69

• there is no difference in the number of FoxP3 regulatory T cells found at the site of inflammation when compared to control mice suggesting the inability to make IL-10 is affecting regulator T cell function

decreased interleukin-10 secretion ( J:134513 )

• IL-10 producing T cells are greatly diminished by the Foxp3-expressing, but not Foxp3 negative, CD4⁺ T cell subset

• there is almost a two-fold reduction in the number of lamina propria Foxp3 negative lymphocytes that produce IL-10

lung inflammation ( J:134513 )

• mice have mild perivasculitis and moderate mononuclear infiltration around large airways in the lungs

• inducing lung inflammation by sensitizing and then challenging mice with OVA peptide leads to more pronounced inflammatory response with 2.7 fold increase in leukocytes numbers from bronchoalveolar lavage fluid

• OVA induced inflammation leads to markedly increased mucus production, goblet cell expansion, edema, and increased eosinophilic infiltration around major airways

• OVA treated mice also show a marked increase in airway hyperreactivity to aerosolized methacholine

respiratory system

lung inflammation ( J:134513 )

• mice have mild perivasculitis and moderate mononuclear infiltration around large airways in the lungs

• OVA induced inflammation leads to markedly increased mucus production, goblet cell expansion, edema, and increased eosinophilic infiltration around major airways

• OVA treated mice also show a marked increase in airway hyperreactivity to aerosolized methacholine

hematopoietic system

abnormal regulatory T cell physiology ( J:134513 )

• Foxp3 expressing regulatory CD4 T cells fail to produce IL-10

• mice exhibit spontaneous colitis and inflammation in the lung that is contributable to defective regulatory T cell responses

• mice also have heightened inflammatory responses when challenged with antigen to the lungs or skin

Allelic Composition	Itch^tm1.1Alta/Itch^tm1.1Alta Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Itch^tm1.1Alta mutation (0 available); any Itch mutation (92 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:204685 )

• from 6 weeks of age

immune system

immune system phenotype ( J:204685 )

N	• mice exhibit normal response to experimental autoimmune encephalomyelitis

abnormal lymphopoiesis ( J:204685 )

• from 6 weeks of age, mice exhibit a lymphoproliferative disorder unlike wild-type mice

abnormal T-helper 2 cell differentiation ( J:204685 )

• increased when induced by regulatory T cells

• however, anti-IL4 antibody abolishes Th2 instruction

decreased CD4-positive, alpha-beta T cell number ( J:204685 )

• slightly decreased proportion in the spleen

increased leukocyte cell number ( J:204685 )

• in splenic red pulp

increased eosinophil cell number ( J:204685 )

• in the bronchoalveolar lavage fluid of ovalbumin-treated mice

increased CD4-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased CD8-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased regulatory T cell number ( J:204685 )

• slightly

abnormal immunoglobulin level ( J:204685 )

increased IgA level ( J:204685 )

• modestly

increased IgE level ( J:204685 )

• ovalbumin-specific IgE in ovalbumin-treated mice

increased IgG1 level ( J:204685 )

abnormal T-helper 2 physiology ( J:204685 )

• CD4+ T cells and anti-CD3-stimulated splenocytes produce more Th2 cytokines (IL4, IL5 and IL10) compared with wild-type mice

abnormal regulatory T cell physiology ( J:204685 )

• when injected into ovalbumin-immunized wild-type mice, regulatory T cells exacerbate Th2 immune responses in allergic reaction compared with wild-type cells

• however, regulatory T cells exhibit normal suppressive functions and stability

increased regulatory T cell apoptosis ( J:204685 )

• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells

abnormal lymph organ size ( J:204685 )

• from 6 weeks of age, mice exhibit larger size and cellularity of peripheral lymphoid organs compared with wild-type mice

enlarged spleen ( J:204685 )

enlarged lymph nodes ( J:204685 )

abnormal interleukin secretion ( J:204685 )

increased interleukin-10 secretion ( J:204685 )

• from CD4+ T cells

• from anti-CD3-stimulated splenocytes

increased interleukin-13 secretion ( J:204685 )

• from anti-CD3-stimulated splenocytes

increased interleukin-17 secretion ( J:204685 )

• from CD4+ T cells in the lungs

increased interleukin-4 secretion ( J:204685 )

• from CD4+ T cells

• from anti-CD3-stimulated splenocytes

increased interleukin-5 secretion ( J:204685 )

• from CD4+ T cells

• from anti-CD3-stimulated splenocytes

increased inflammatory response ( J:204685 )

• massive infiltration in the lungs, stomach, skin and liver

• however, no lesions are observed in the kidney and colon

lung inflammation ( J:204685 )

• from 6 weeks of age

• more severe when induced by ovalbumin with increased total cells and eosinophils in the bronchoalveolar lavage fluid

dermatitis ( J:204685 )

• severe at 16 weeks

integument

dermatitis ( J:204685 )

• severe at 16 weeks

skin lesions ( J:204685 )

• from 6 weeks of age

respiratory system

lung inflammation ( J:204685 )

• from 6 weeks of age

• more severe when induced by ovalbumin with increased total cells and eosinophils in the bronchoalveolar lavage fluid

growth/size/body

weight loss ( J:204685 )

• from 6 weeks of age

enlarged spleen ( J:204685 )

cellular

increased regulatory T cell apoptosis ( J:204685 )

• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells

hematopoietic system

enlarged spleen ( J:204685 )

abnormal lymphopoiesis ( J:204685 )

• from 6 weeks of age, mice exhibit a lymphoproliferative disorder unlike wild-type mice

abnormal T-helper 2 cell differentiation ( J:204685 )

• increased when induced by regulatory T cells

• however, anti-IL4 antibody abolishes Th2 instruction

decreased CD4-positive, alpha-beta T cell number ( J:204685 )

• slightly decreased proportion in the spleen

increased leukocyte cell number ( J:204685 )

• in splenic red pulp

increased eosinophil cell number ( J:204685 )

• in the bronchoalveolar lavage fluid of ovalbumin-treated mice

increased CD4-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased CD8-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased regulatory T cell number ( J:204685 )

• slightly

abnormal immunoglobulin level ( J:204685 )

increased IgA level ( J:204685 )

• modestly

increased IgE level ( J:204685 )

• ovalbumin-specific IgE in ovalbumin-treated mice

increased IgG1 level ( J:204685 )

abnormal T-helper 2 physiology ( J:204685 )

• CD4+ T cells and anti-CD3-stimulated splenocytes produce more Th2 cytokines (IL4, IL5 and IL10) compared with wild-type mice

abnormal regulatory T cell physiology ( J:204685 )

• when injected into ovalbumin-immunized wild-type mice, regulatory T cells exacerbate Th2 immune responses in allergic reaction compared with wild-type cells

• however, regulatory T cells exhibit normal suppressive functions and stability

increased regulatory T cell apoptosis ( J:204685 )

• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells

Allelic Composition	Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli} mutation (1 available); any Gt(ROSA)26Sor mutation (994 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:189962 )

N	• mice exhibit normal regulatory T cell differentiation and homeostasis

Allelic Composition	Itch^tm1.1Alta/Itch^tm1.1Alta Gt(ROSA)26Sor^tm1Hjf/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sor^tm1Hjf mutation (5 available); any Gt(ROSA)26Sor mutation (994 available)
Itch^tm1.1Alta mutation (0 available); any Itch mutation (92 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:204685 )

N	• regulatory T cells exhibit normal suppressive functions and stability

increased inflammatory response ( J:204685 )

• severe

Allelic Composition	Becn1^tm1Smoc/Becn1^tm1Smoc Zfp91^tm1Smoc/Zfp91^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Becn1^tm1Smoc mutation (1 available); any Becn1 mutation (36 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Zfp91^tm1Smoc mutation (0 available); any Zfp91 mutation (97 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit higher baseline and maximum glycolytic rates than wild-type T reg cells but similar to either single conditional deletion mice

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression than wild-type T reg cells but similar to either single conditional deletion mice

immune system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit higher baseline and maximum glycolytic rates than wild-type T reg cells but similar to either single conditional deletion mice

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression than wild-type T reg cells but similar to either single conditional deletion mice

Allelic Composition	Foxp3^{tm4(YFP/icre)Ayr}/Y Zfp91^tm1Smoc/Zfp91^tm1Smoc
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Zfp91^tm1Smoc mutation (0 available); any Zfp91 mutation (97 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased susceptibility to induced colitis ( J:306129 )

• mice show increased susceptibility to dextran sodium sulfate (DSS)-induced acute colitis, showing rapid body weight loss, increased clinical scores, exacerbated colon length shortening, increased inflammatory cells into the colon

increased CD8-positive, alpha-beta T cell number ( J:306129 )

• mice show elevated IFN-gamma-producing CD8+ effector T cell proportions in the spleen and mesenteric lymph nodes at 10 weeks of age

decreased regulatory T cell number ( J:306129 )

• mice show low regulatory T (T reg) cell percentages in the spleen and mesenteric lymph nodes at 10 months of age

• however, T cell development and peripheral T cell homeostasis is not affected and T reg cell percentages in the thymus, spleen, and peripheral lymph nodes are normal in 6-week-old mice

abnormal regulatory T cell physiology ( J:306129 )

• adoptive transfer of mutant T reg cells with naive CD45RB^hiCD4+ T cells into RAG-1 deficient mice results in gradual weight loss and a greater frequency of IFN-gamma-producing effector CD4+ T cells that is not seen with wild-type T reg cells

• the fraction of FoxP3+ cells from mice transferred with mutant T reg cells and naive CD45RB^hiCD4+ T cells is decreased compared with those from mice transferred with wild-type T reg cells

• mutant T reg cells transferred into RAG-1 deficient mice cause gradual weight loss, show a more profound loss of Foxp3 expression, and the FoxP3-mainting fraction of mutant T reg cells produce higher amounts of IFN-gamma

• T reg cells exhibit higher baseline and maximum glycolytic rates (increased extracellular acidification rate), indicating hyperglycolysis, however baseline and maximum OXPHOS rates (increased mitochondrial oxygen consumption rate) are not affected

• T regs treated with dichloroacetic acid, which shifts glycolysis towards OXPHOS, show diminished IFN-gamma production

• treatment of stimulated T reg cells with the mTORC1 inhibitor rapamycin lowers the extracellular acidification rate and diminishes IFN-gamma production

• autophagy levels are decreased in T reg cells and autophagy induction after TCR stimulation is severely, but not completely, blocked in T reg cells

increased susceptibility to autoimmune disorder ( J:306129 )

• mice exhibit severe autoimmune symptoms with lymphocytic infiltration in many nonlymphoid organs at 10 months of age

digestive/alimentary system

increased susceptibility to induced colitis ( J:306129 )

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:306129 )

• mice show elevated IFN-gamma-producing CD8+ effector T cell proportions in the spleen and mesenteric lymph nodes at 10 weeks of age

decreased regulatory T cell number ( J:306129 )

• mice show low regulatory T (T reg) cell percentages in the spleen and mesenteric lymph nodes at 10 months of age

• however, T cell development and peripheral T cell homeostasis is not affected and T reg cell percentages in the thymus, spleen, and peripheral lymph nodes are normal in 6-week-old mice

abnormal regulatory T cell physiology ( J:306129 )

• the fraction of FoxP3+ cells from mice transferred with mutant T reg cells and naive CD45RB^hiCD4+ T cells is decreased compared with those from mice transferred with wild-type T reg cells

• T regs treated with dichloroacetic acid, which shifts glycolysis towards OXPHOS, show diminished IFN-gamma production

• treatment of stimulated T reg cells with the mTORC1 inhibitor rapamycin lowers the extracellular acidification rate and diminishes IFN-gamma production

• autophagy levels are decreased in T reg cells and autophagy induction after TCR stimulation is severely, but not completely, blocked in T reg cells

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:306129 )

• mice show increased susceptibility to azoxymethane and DSS-induced colitis-associated cancer, showing higher tumor numbers in the colon than wild-type mice

neoplasm

increased incidence of tumors by chemical induction ( J:306129 )

• mice show increased susceptibility to azoxymethane and DSS-induced colitis-associated cancer, showing higher tumor numbers in the colon than wild-type mice

Allelic Composition	Becn1^tm1Smoc/Becn1^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit increased extracellular acidification rate indicating higher baseline and maximum glycolytic rates than wild-type T reg cells

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression

immune system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit increased extracellular acidification rate indicating higher baseline and maximum glycolytic rates than wild-type T reg cells

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression

Allelic Composition	Cd28^tm1Ltu/Cd28^tm1Ltu Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd28^tm1Ltu mutation (1 available); any Cd28 mutation (52 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Skin inflammation and enlarged lymph nodes and spleen in Cd28^tm1Ltu/Cd28^tm1Ltu Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺ mice

immune system

decreased T cell proliferation ( J:194503 )

• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes

enlarged spleen ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

increased B cell number ( J:194503 )

• in the lymph node

decreased regulatory T cell number ( J:194503 )

• in the thymus but not lymph node or spleens

• in the thymus and periphery of female mice at 3 months

increased activated T cell number ( J:194503 )

• in the lymph nodes and spleen at 2 to 5 months

• with IFN-gamma expression

enlarged lymph nodes ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

increased susceptibility to autoimmune disorder ( J:194503 )

• by 8 to 12 weeks with crusting eyelids, facial hair loss, which progressed to hair loss on the trunk, skin lesions, an ill appearance, ruffled fur, hunching and reduced movements

• however, CD28-sufficient regulatory T cells prevent the autoimmune disease development

increased susceptibility to experimental autoimmune encephalomyelitis ( J:194503 )

• 20 days after immunization, mice treated with rMOG/CFA plus pertussis toxin exhibit increased clinical score and fail to recover compared with wild-type mice

skin inflammation ( J:194503 )

• at 2 to 5 months

integument

skin inflammation ( J:194503 )

• at 2 to 5 months

focal hair loss ( J:194503 )

• facial hair loss, which progressed to hair loss on the trunk by 8 to 12 weeks

ruffled hair ( J:194503 )

• by 8 to 12 weeks

skin lesions ( J:194503 )

• by 8 to 12 weeks

behavior/neurological

hunched posture ( J:194503 )

• by 8 to 12 weeks

decreased locomotor activity ( J:194503 )

• by 8 to 12 weeks

vision/eye

abnormal eyelid morphology ( J:194503 )

• crusting by 8 to 12 weeks

hematopoietic system

decreased T cell proliferation ( J:194503 )

• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes

enlarged spleen ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

increased B cell number ( J:194503 )

• in the lymph node

decreased regulatory T cell number ( J:194503 )

• in the thymus but not lymph node or spleens

• in the thymus and periphery of female mice at 3 months

increased activated T cell number ( J:194503 )

• in the lymph nodes and spleen at 2 to 5 months

• with IFN-gamma expression

growth/size/body

enlarged spleen ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

cellular

decreased T cell proliferation ( J:194503 )

• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes

Allelic Composition	Nr4a1^tm1Pcn/Nr4a1^tm1Pcn Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Nr4a1^tm1Pcn mutation (0 available); any Nr4a1 mutation (39 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:205658 )

N	• the ratio of YFP+/YFP- regulatory T cells is normal

Allelic Composition	Myd88^tm1.1Medz/Myd88^tm1.1Medz Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Myd88^tm1.1Medz mutation (0 available); any Myd88 mutation (52 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:209373 )

N	• mice mount CD4+ T cell responses in response to immunization with OVA and LPS that are indistinguishable from wild-type mice

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-Il-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-IL-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Allelic Composition	Altre^tm1.1Hbgl/Altre^tm1.1Hbgl Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Altre^tm1.1Hbgl mutation (0 available); any Altre mutation (15 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:342907 )

• reduced survival rate in aged mice

immune system

increased CD8-positive, alpha-beta T cell number ( J:342907 )

• increase in the CD8+ T cell subset in the spleen and liver in aged mice

increased memory T cell number ( J:342907 )

• increase in the percentage of effector memory (CD62L- CD44+) T cells in aged mice

decreased T cell number ( J:342907 )

• decrease in the percentage of naive (CD62L+ CD44-) T cells in aged mice

• difference is more pronounced in the CD8+ T cell compartment

decreased regulatory T cell number ( J:342907 )

• significant decrease in the percentage of T reg cells in the live and slight reduction in the spleen at 14 months of age

• however, no change in T reg percentage is seen in young mice

abnormal regulatory T cell physiology ( J:342907 )

• transfection of T reg cells from aged mice into Rag null mice indicates that the ability of these cells to control T cell proliferation is impaired

• oxygen consumption rate is markedly suppressed in aged but not young T reg cells indicating mitochondrial dysfunction

increased regulatory T cell apoptosis ( J:342907 )

• substantial increase in the liver and smaller increase in the spleen at 14 months of age

increased interleukin-17 secretion ( J:342907 )

• in aged mice

neoplasm

increased liver tumor incidence ( J:342907 )

• at 18 months of age

homeostasis/metabolism

increased circulating alanine transaminase level ( J:342907 )

• at 14 months of age

liver/biliary system

abnormal liver morphology ( J:342907 )

• aged mice (14 months) show more severe hepatic damage compared to age matched controls

increased liver tumor incidence ( J:342907 )

• at 18 months of age

increased susceptibility to diet-induced hepatic steatosis ( J:342907 )

• increase in steatotic hepatocytes, inflammation and fat accumulation in the liver following 8 months on a high fat diet

liver fibrosis ( J:342907 )

• increased at 14 months of age compared to age matched controls

cellular

abnormal mitochondrial morphology ( J:342907 )

• decreased mitochondrial mass in aged T reg cells

decreased mitochondrial size ( J:342907 )

• decreased length of mitochondria in T reg cells from aged mice

increased regulatory T cell apoptosis ( J:342907 )

• substantial increase in the liver and smaller increase in the spleen at 14 months of age

abnormal mitochondrial physiology ( J:342907 )

• decrease in mitochondrial membrane potential in aged but not young T reg cells

• increase in reactive oxygen species in aged but not young T reg cells

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:342907 )

• increase in the CD8+ T cell subset in the spleen and liver in aged mice

increased memory T cell number ( J:342907 )

• increase in the percentage of effector memory (CD62L- CD44+) T cells in aged mice

decreased T cell number ( J:342907 )

• decrease in the percentage of naive (CD62L+ CD44-) T cells in aged mice

• difference is more pronounced in the CD8+ T cell compartment

decreased regulatory T cell number ( J:342907 )

• significant decrease in the percentage of T reg cells in the live and slight reduction in the spleen at 14 months of age

• however, no change in T reg percentage is seen in young mice

abnormal regulatory T cell physiology ( J:342907 )

• transfection of T reg cells from aged mice into Rag null mice indicates that the ability of these cells to control T cell proliferation is impaired

• oxygen consumption rate is markedly suppressed in aged but not young T reg cells indicating mitochondrial dysfunction

increased regulatory T cell apoptosis ( J:342907 )

• substantial increase in the liver and smaller increase in the spleen at 14 months of age

Allelic Composition	Pgd^tm1.1Pse/Pgd^tm1.1Pse Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Pgd^tm1.1Pse mutation (0 available); any Pgd mutation (21 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:313817 )

• shorter than normal lifespans

immune system

enlarged spleen ( J:313817 )

ear inflammation ( J:313817 )

• crusting ears

increased T cell number ( J:313817 )

• increased CD4/CD8 positive in the spleen

• increased frequencies of CD44^high increased CD62L^low effector/memory T cells with reduced CD44^low CD62L^high

abnormal CD4-positive helper T cell morphology ( J:313817 )

• increased Th1, Th2, and Th17 responses.

increased IgA level ( J:313817 )

increased IgD level ( J:313817 )

increased IgE level ( J:313817 )

increased IgG2a level ( J:313817 )

increased IgG3 level ( J:313817 )

increased IgM level ( J:313817 )

abnormal regulatory T cell physiology ( J:313817 )

• reduced Tregs suppressive function

increased circulating interferon-gamma level ( J:313817 )

increased circulating interleukin-17 level ( J:313817 )

increased circulating interleukin-4 level ( J:313817 )

increased circulating interleukin-5 level ( J:313817 )

enlarged lymph nodes ( J:313817 )

increased susceptibility to type I hypersensitivity reaction ( J:313817 )

• significant Th2 (allergic) responses in the lung and spleen with increased collagen deposits

increased interferon-gamma secretion ( J:313817 )

• from CD4+ and CD8+ T cells

blepharitis ( J:313817 )

• crusting eyelids

growth/size/body

decreased body size ( J:313817 )

enlarged spleen ( J:313817 )

homeostasis/metabolism

increased circulating interferon-gamma level ( J:313817 )

increased circulating interleukin-17 level ( J:313817 )

increased circulating interleukin-4 level ( J:313817 )

increased circulating interleukin-5 level ( J:313817 )

hearing/vestibular/ear

ear inflammation ( J:313817 )

• crusting ears

vision/eye

blepharitis ( J:313817 )

• crusting eyelids

hematopoietic system

enlarged spleen ( J:313817 )

increased T cell number ( J:313817 )

• increased CD4/CD8 positive in the spleen

• increased frequencies of CD44^high increased CD62L^low effector/memory T cells with reduced CD44^low CD62L^high

abnormal CD4-positive helper T cell morphology ( J:313817 )

• increased Th1, Th2, and Th17 responses.

increased IgA level ( J:313817 )

increased IgD level ( J:313817 )

increased IgE level ( J:313817 )

increased IgG2a level ( J:313817 )

increased IgG3 level ( J:313817 )

increased IgM level ( J:313817 )

abnormal regulatory T cell physiology ( J:313817 )

• reduced Tregs suppressive function

Allelic Composition	Areg^{tm2c(EUCOMM)Hmgu}/Areg^{tm2c(EUCOMM)Hmgu} Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Areg^{tm2c(EUCOMM)Hmgu} mutation (0 available); any Areg mutation (29 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:224904 )

N	• contrary to germ line deletion, mice are born in Mendelian ratios with no gender bias

immune system

immune system phenotype ( J:224904 )

N	• mice exhibit normal frequencies and numbers of B and T cells and activated T cells • unchallenged mice exhibit no heightened inflammatory response and normal T regulator cell suppressor function

increased susceptibility to Orthomyxoviridae infection ( J:224904 )

• flu-infected mice exhibit a greater decrease in average blood oxygen saturation, overall lung tissue damage and decreased surface temperature compared with wild-type mice

• however, flu-infected mice exhibit normal T cell activation and T regulatory cell mediated suppression of anti-vial immunity

homeostasis/metabolism

decreased blood oxygen saturation level ( J:224904 )

• flu-infected mice exhibit a greater decrease in average blood oxygen saturation compared with wild-type mice

• however, mice do not exhibit anemia

decreased body surface temperature ( J:224904 )

• in flu-infected mice

• however, core body temperature is normal

Allelic Composition	Bcl2l11^tm6.1Boui/Bcl2l11^tm6.1Boui Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bcl2l11^tm6.1Boui mutation (1 available); any Bcl2l11 mutation (36 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

increased regulatory T cell number ( J:208318 )

• increased frequency at 2 months that may be due to increased thymic output

• further increase at 6 months as in Bcl2l11^tm1.1Ast homozygotes

immune system

increased regulatory T cell number ( J:208318 )

• increased frequency at 2 months that may be due to increased thymic output

• further increase at 6 months as in Bcl2l11^tm1.1Ast homozygotes

Allelic Composition	Cish^tm1.1Cdon/Cish^tm1.1Cdon Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cish^tm1.1Cdon mutation (0 available); any Cish mutation (23 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:204829 )

N	• mice exhibit normal asthmatic response

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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