All Phenotypes Foxp3<tm4(YFP/icre)Ayr> MGI Mouse

About Help FAQ

Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Foxp3^{tm4(YFP/icre)Ayr}
targeted mutation 4, Alexander Y Rudensky
MGI:3790499

Summary

28 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Socs1^tm1Ayos/Socs1^tm1Ayos Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:4887825
cn2	Foxo1^tm1Flv/Foxo1^tm1Flv Ifng^tm1Ts/Ifng^tm1Ts Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ	MGI:5448156
cn3	B9d2/Tgfb1^tm1Flv/B9d2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:4943575
cn4	Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:5448157
cn5	B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:4943576
cn6	Foxo1^tm1Flv/Foxo1^tm1Flv Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6	MGI:5448151
cn7	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺ Nr4a2^tm1.1Pcn/Nr4a2^tm1.1Pcn	involves: 129S1/Sv * 129X1/SvJ	MGI:5547376
cn8	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr} Ikzf4^tm1Djr/Ikzf4^tm1Djr	involves: 129S1/Sv * 129X1/SvJ	MGI:6467272
cn9	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}	involves: 129S1/Sv * 129X1/SvJ	MGI:3806256
cn10	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ	MGI:3806255
cn11	Nr4a2^tm1.1Pcn/Nr4a2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ	MGI:5547375
cn12	Il10^tm1Roer/Il10^tm1Roer Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3790650
cn13	Itch^tm1.1Alta/Itch^tm1.1Alta Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5563103
cn14	Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5448149
cn15	Itch^tm1.1Alta/Itch^tm1.1Alta Gt(ROSA)26Sor^tm1Hjf/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5563102
cn16	Becn1^tm1Smoc/Becn1^tm1Smoc Zfp91^tm1Smoc/Zfp91^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:7645512
cn17	Foxp3^{tm4(YFP/icre)Ayr}/Y Zfp91^tm1Smoc/Zfp91^tm1Smoc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:7645491
cn18	Becn1^tm1Smoc/Becn1^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:7645511
cn19	Cd28^tm1Ltu/Cd28^tm1Ltu Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5485238
cn20	Nr4a1^tm1Pcn/Nr4a1^tm1Pcn Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5546602
cn21	Myd88^tm1.1Medz/Myd88^tm1.1Medz Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5616083
cn22	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806254
cn23	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806253
cn24	Altre^tm1.1Hbgl/Altre^tm1.1Hbgl Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:7562295
cn25	Pgd^tm1.1Pse/Pgd^tm1.1Pse Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:7657738
cn26	Areg^{tm2c(EUCOMM)Hmgu}/Areg^{tm2c(EUCOMM)Hmgu} Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:5806476
cn27	Bcl2l11^tm6.1Boui/Bcl2l11^tm6.1Boui Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5562712
cn28	Cish^tm1.1Cdon/Cish^tm1.1Cdon Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺	involves: 129S/Sv * 129X1/SvJ * C57BL/6	MGI:5544440

Allelic Composition	Socs1^tm1Ayos/Socs1^tm1Ayos Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Socs1^tm1Ayos mutation (1 available); any Socs1 mutation (30 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased regulatory T cell number ( J:167922 )

blepharitis ( J:167922 )

• by 6 weeks of age

conjunctivitis ( J:167922 )

• by 6 weeks of age

dermatitis ( J:167922 )

• by 6 weeks of age

integument

dermatitis ( J:167922 )

• by 6 weeks of age

vision/eye

blepharitis ( J:167922 )

• by 6 weeks of age

conjunctivitis ( J:167922 )

• by 6 weeks of age

hematopoietic system

increased regulatory T cell number ( J:167922 )

Allelic Composition	Foxo1^tm1Flv/Foxo1^tm1Flv Ifng^tm1Ts/Ifng^tm1Ts Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1^tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Ifng^tm1Ts mutation (18 available); any Ifng mutation (49 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:189962 )

N	• the lethal inflammatory phenotype is partially rescued

immune system

immune system phenotype ( J:189962 )

N	• regulatory T cells are able to suppress development of colitis in Rag1 null mice receiving naive T cells

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/cre)Ayr}/Foxp3⁺ mice

Allelic Composition	B9d2/Tgfb1^tm1Flv/B9d2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B9d2/Tgfb1^tm1Flv mutation (0 available); any B9d2 mutation (13 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice • mice exhibit normal susceptibility to experimental autoimmune encephalomyelitis and Th17 cell differentiation

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

growth/size/body

growth/size/body region phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice

hematopoietic system

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

Allelic Composition	Foxo1^tm1Flv/Foxo1^tm1Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:189962 )

• moribund within 35 days of birth

immune system

increased T cell proliferation ( J:189962 )

enlarged spleen ( J:189962 )

increased T cell number ( J:189962 )

• in the spleen and peripheral lymph node

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased regulatory T cell number ( J:189962 )

• in peripheral lymph node at day 20

• however, thymic and splenic regulatory T cell numbers are normal at day 12

abnormal regulatory T cell physiology ( J:189962 )

• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells

enlarged lymph nodes ( J:189962 )

increased interferon-gamma secretion ( J:189962 )

abnormal inflammatory response ( J:189962 )

• infiltrate of leukocytes in the salivary glands, lung interstitia, liver sinusoids, pancreas acini, stomach and colon mucosa

behavior/neurological

hunched posture ( J:189962 )

• lack of mobility associated with hunched posture

paralysis ( J:189962 )

• lack of mobility associated with hunched posture

integument

abnormal skin appearance ( J:189962 )

• crusting of the ears, eyelids and tail

limbs/digits/tail

abnormal tail morphology ( J:189962 )

• scurfy tail

hematopoietic system

increased T cell proliferation ( J:189962 )

enlarged spleen ( J:189962 )

increased T cell number ( J:189962 )

• in the spleen and peripheral lymph node

increased CD4-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased CD8-positive, alpha-beta T cell number ( J:189962 )

• in the spleen and peripheral lymph nodes

increased regulatory T cell number ( J:189962 )

• in peripheral lymph node at day 20

• however, thymic and splenic regulatory T cell numbers are normal at day 12

abnormal regulatory T cell physiology ( J:189962 )

• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells

growth/size/body

enlarged spleen ( J:189962 )

cellular

increased T cell proliferation ( J:189962 )

Allelic Composition	B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B9d2/Tgfb1^tm1Flv mutation (0 available); any Tgfb1 mutation (35 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Tgfb1^tm2Flv mutation (0 available); any Tgfb1 mutation (35 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

growth/size/body region phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice

hematopoietic system

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

immune system

immune system phenotype ( J:169839 )

N	• mice do not exhibit wasting or inflammation unlike B9d2/Tgfb1^tm1Flv/Tgfb1^tm2Flv Tg(Cd4-cre)1Cwi mice • mice exhibit normal susceptibility to experimental autoimmune encephalomyelitis and Th17 cell differentiation

increased regulatory T cell number ( J:169839 )

• in the peripheral and mesenteric lymph nodes, but not the spleen

• 2-fold increase in mesenteric regulatory T cell

Allelic Composition	Foxo1^tm1Flv/Foxo1^tm1Flv Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1^tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli} mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:189962 )

N	• the lethal inflammatory phenotype is completely rescued

immune system

immune system phenotype ( J:189962 )

N	• the lethal inflammatory phenotype is completely rescued

Allelic Composition	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺ Nr4a2^tm1.1Pcn/Nr4a2^tm1.1Pcn
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Nr4a2^tm1.1Pcn mutation (0 available); any Nr4a2 mutation (44 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal regulatory T cell morphology ( J:205658 )

• regulatory T cells are not properly maintained

abnormal regulatory T cell physiology ( J:205658 )

• slightly attenuated suppressive activity

• in a transfer model of colitis, regulatory T cells fail to inhibit wasting disease and colitis compared with wild-type cells

hematopoietic system

abnormal regulatory T cell morphology ( J:205658 )

• regulatory T cells are not properly maintained

abnormal regulatory T cell physiology ( J:205658 )

• slightly attenuated suppressive activity

• in a transfer model of colitis, regulatory T cells fail to inhibit wasting disease and colitis compared with wild-type cells

Allelic Composition	Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr} Ikzf4^tm1Djr/Ikzf4^tm1Djr
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Ikzf4^tm1Djr mutation (0 available); any Ikzf4 mutation (33 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

small intestinal inflammation ( J:294549 )

• increased lymphocyte infiltration and neutrophils in lamina propria at age 3 months

enlarged spleen ( J:294549 )

• slight splenomegaly at age 2 months, marked splenomegaly at age 3 months

increased germinal center B cell number ( J:294549 )

• increased percentage in immunized and unimmunized mice at age 3 months

decreased regulatory T cell number ( J:294549 )

• reduced percentage of regulatory follicular T cells in immunized and unimmunized mice at age 3 months

increased T cell number ( J:294549 )

• increased absolute total CD4+, CD8+ and regulatory T cell numbers at age 3 months

• elevated percentage of regulatory T cells at age 3 months

• elevated percentage of CD44hi-CD62Llo CD4+, CD8+ and regulatory T cells at age 3 months

• increased percentage of follicular T cells in unimmunized mice at age 3 months

increased T follicular helper cell number ( J:294549 )

• increased percentage in unimmunized mice at age 3 months

increased spleen B cell follicle number ( J:294549 )

• increased number in immunized mice at age 3 months

increased spleen germinal center number ( J:294549 )

• increased number in immunized mice at age 3 months

increased immunoglobulin level ( J:294549 )

• increased serum levels of all immunoglobulin isotypes and anti-nuclear antibodies (ANA) at age 3 months

enlarged lymph nodes ( J:294549 )

• slight lymphadenopathy at age 2 months, marked lymphadenopathy at age 3 months

increased susceptibility to experimental autoimmune encephalomyelitis ( J:294549 )

• at age 3 months

chronic inflammation ( J:294549 )

• increased lymphocyte infiltration in small intestine, lungs, liver, salivary glands and kidneys at age 3 months

granulomatous inflammation ( J:294549 )

• hepatic microgranulomas at age 3 months

glomerulonephritis ( J:294549 )

• at age 3 months

hematopoietic system

enlarged spleen ( J:294549 )

• slight splenomegaly at age 2 months, marked splenomegaly at age 3 months

increased germinal center B cell number ( J:294549 )

• increased percentage in immunized and unimmunized mice at age 3 months

decreased regulatory T cell number ( J:294549 )

• reduced percentage of regulatory follicular T cells in immunized and unimmunized mice at age 3 months

increased T cell number ( J:294549 )

• increased absolute total CD4+, CD8+ and regulatory T cell numbers at age 3 months

• elevated percentage of regulatory T cells at age 3 months

• elevated percentage of CD44hi-CD62Llo CD4+, CD8+ and regulatory T cells at age 3 months

• increased percentage of follicular T cells in unimmunized mice at age 3 months

increased T follicular helper cell number ( J:294549 )

• increased percentage in unimmunized mice at age 3 months

increased spleen B cell follicle number ( J:294549 )

• increased number in immunized mice at age 3 months

increased spleen germinal center number ( J:294549 )

• increased number in immunized mice at age 3 months

increased immunoglobulin level ( J:294549 )

• increased serum levels of all immunoglobulin isotypes and anti-nuclear antibodies (ANA) at age 3 months

growth/size/body

enlarged spleen ( J:294549 )

• slight splenomegaly at age 2 months, marked splenomegaly at age 3 months

digestive/alimentary system

abnormal small intestine morphology ( J:294549 )

• hyperplastic epithelium at age 3 months

decreased small intestinal microvillus size ( J:294549 )

• blunted at age 3 months

small intestinal inflammation ( J:294549 )

• increased lymphocyte infiltration and neutrophils in lamina propria at age 3 months

renal/urinary system

glomerulonephritis ( J:294549 )

• at age 3 months

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
encephalomyelitis		DOID:640		J:294549

Allelic Composition	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1^tm1Bdh mutation (4 available); any Dicer1 mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

enlarged lymph nodes ( J:138683 )

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

Allelic Composition	Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

enlarged lymph nodes ( J:138683 )

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

Allelic Composition	Nr4a2^tm1.1Pcn/Nr4a2⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal regulatory T cell morphology ( J:205658 )

• Treg cells are not properly maintained

hematopoietic system

abnormal regulatory T cell morphology ( J:205658 )

• Treg cells are not properly maintained

Allelic Composition	Il10^tm1Roer/Il10^tm1Roer Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Il10^tm1Roer mutation (1 available); any Il10 mutation (45 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

colitis ( J:134513 )

• starting at 10 weeks of age, mice present with the gross manifestations of colitis including colonic thickening and substantial increase in the size of mesenteric lymph nodes

• histological examinations reveals prominent mononuclear infiltration of epithelial tissues as well as tissue destruction of the cecum and portions of the colon

• disease is similar to what is observed in Il10^tm1Cgn null mice though with less severity and incidence, and a slightly later onset

immune system

colitis ( J:134513 )

• starting at 10 weeks of age, mice present with the gross manifestations of colitis including colonic thickening and substantial increase in the size of mesenteric lymph nodes

• histological examinations reveals prominent mononuclear infiltration of epithelial tissues as well as tissue destruction of the cecum and portions of the colon

• disease is similar to what is observed in Il10^tm1Cgn null mice though with less severity and incidence, and a slightly later onset

abnormal regulatory T cell physiology ( J:134513 )

• Foxp3 expressing regulatory CD4 T cells fail to produce IL-10

• mice exhibit spontaneous colitis and inflammation in the lung that is contributable to defective regulatory T cell responses

• mice also have heightened inflammatory responses when challenged with antigen to the lungs or skin

abnormal type IV hypersensitivity reaction ( J:134513 )

• there is a substantial increase in ear thickness and inflammation after application of dinitrofluorobenzene (DNFB) on the ear

• T cells infiltrating the site of DNFB application have higher expression of the activation marker CD69

• there is no difference in the number of FoxP3 regulatory T cells found at the site of inflammation when compared to control mice suggesting the inability to make IL-10 is affecting regulator T cell function

decreased interleukin-10 secretion ( J:134513 )

• IL-10 producing T cells are greatly diminished by the Foxp3-expressing, but not Foxp3 negative, CD4⁺ T cell subset

• there is almost a two-fold reduction in the number of lamina propria Foxp3 negative lymphocytes that produce IL-10

lung inflammation ( J:134513 )

• mice have mild perivasculitis and moderate mononuclear infiltration around large airways in the lungs

• inducing lung inflammation by sensitizing and then challenging mice with OVA peptide leads to more pronounced inflammatory response with 2.7 fold increase in leukocytes numbers from bronchoalveolar lavage fluid

• OVA induced inflammation leads to markedly increased mucus production, goblet cell expansion, edema, and increased eosinophilic infiltration around major airways

• OVA treated mice also show a marked increase in airway hyperreactivity to aerosolized methacholine

respiratory system

lung inflammation ( J:134513 )

• mice have mild perivasculitis and moderate mononuclear infiltration around large airways in the lungs

• OVA induced inflammation leads to markedly increased mucus production, goblet cell expansion, edema, and increased eosinophilic infiltration around major airways

• OVA treated mice also show a marked increase in airway hyperreactivity to aerosolized methacholine

hematopoietic system

abnormal regulatory T cell physiology ( J:134513 )

• Foxp3 expressing regulatory CD4 T cells fail to produce IL-10

• mice exhibit spontaneous colitis and inflammation in the lung that is contributable to defective regulatory T cell responses

• mice also have heightened inflammatory responses when challenged with antigen to the lungs or skin

Allelic Composition	Itch^tm1.1Alta/Itch^tm1.1Alta Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Itch^tm1.1Alta mutation (0 available); any Itch mutation (92 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:204685 )

• from 6 weeks of age

immune system

immune system phenotype ( J:204685 )

N	• mice exhibit normal response to experimental autoimmune encephalomyelitis

abnormal lymphopoiesis ( J:204685 )

• from 6 weeks of age, mice exhibit a lymphoproliferative disorder unlike wild-type mice

abnormal T-helper 2 cell differentiation ( J:204685 )

• increased when induced by regulatory T cells

• however, anti-IL4 antibody abolishes Th2 instruction

decreased CD4-positive, alpha-beta T cell number ( J:204685 )

• slightly decreased proportion in the spleen

increased leukocyte cell number ( J:204685 )

• in splenic red pulp

increased eosinophil cell number ( J:204685 )

• in the bronchoalveolar lavage fluid of ovalbumin-treated mice

increased CD4-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased CD8-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased regulatory T cell number ( J:204685 )

• slightly

abnormal immunoglobulin level ( J:204685 )

increased IgA level ( J:204685 )

• modestly

increased IgE level ( J:204685 )

• ovalbumin-specific IgE in ovalbumin-treated mice

increased IgG1 level ( J:204685 )

abnormal T-helper 2 physiology ( J:204685 )

• CD4+ T cells and anti-CD3-stimulated splenocytes produce more Th2 cytokines (IL4, IL5 and IL10) compared with wild-type mice

abnormal regulatory T cell physiology ( J:204685 )

• when injected into ovalbumin-immunized wild-type mice, regulatory T cells exacerbate Th2 immune responses in allergic reaction compared with wild-type cells

• however, regulatory T cells exhibit normal suppressive functions and stability

increased regulatory T cell apoptosis ( J:204685 )

• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells

abnormal lymph organ size ( J:204685 )

• from 6 weeks of age, mice exhibit larger size and cellularity of peripheral lymphoid organs compared with wild-type mice

enlarged spleen ( J:204685 )

enlarged lymph nodes ( J:204685 )

abnormal interleukin secretion ( J:204685 )

increased interleukin-10 secretion ( J:204685 )

• from CD4+ T cells

• from anti-CD3-stimulated splenocytes

increased interleukin-13 secretion ( J:204685 )

• from anti-CD3-stimulated splenocytes

increased interleukin-17 secretion ( J:204685 )

• from CD4+ T cells in the lungs

increased interleukin-4 secretion ( J:204685 )

• from CD4+ T cells

• from anti-CD3-stimulated splenocytes

increased interleukin-5 secretion ( J:204685 )

• from CD4+ T cells

• from anti-CD3-stimulated splenocytes

increased inflammatory response ( J:204685 )

• massive infiltration in the lungs, stomach, skin and liver

• however, no lesions are observed in the kidney and colon

lung inflammation ( J:204685 )

• from 6 weeks of age

• more severe when induced by ovalbumin with increased total cells and eosinophils in the bronchoalveolar lavage fluid

dermatitis ( J:204685 )

• severe at 16 weeks

integument

dermatitis ( J:204685 )

• severe at 16 weeks

skin lesions ( J:204685 )

• from 6 weeks of age

respiratory system

lung inflammation ( J:204685 )

• from 6 weeks of age

• more severe when induced by ovalbumin with increased total cells and eosinophils in the bronchoalveolar lavage fluid

growth/size/body

weight loss ( J:204685 )

• from 6 weeks of age

enlarged spleen ( J:204685 )

cellular

increased regulatory T cell apoptosis ( J:204685 )

• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells

hematopoietic system

enlarged spleen ( J:204685 )

abnormal lymphopoiesis ( J:204685 )

• from 6 weeks of age, mice exhibit a lymphoproliferative disorder unlike wild-type mice

abnormal T-helper 2 cell differentiation ( J:204685 )

• increased when induced by regulatory T cells

• however, anti-IL4 antibody abolishes Th2 instruction

decreased CD4-positive, alpha-beta T cell number ( J:204685 )

• slightly decreased proportion in the spleen

increased leukocyte cell number ( J:204685 )

• in splenic red pulp

increased eosinophil cell number ( J:204685 )

• in the bronchoalveolar lavage fluid of ovalbumin-treated mice

increased CD4-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased CD8-positive, alpha-beta T cell number ( J:204685 )

• absolute numbers

increased regulatory T cell number ( J:204685 )

• slightly

abnormal immunoglobulin level ( J:204685 )

increased IgA level ( J:204685 )

• modestly

increased IgE level ( J:204685 )

• ovalbumin-specific IgE in ovalbumin-treated mice

increased IgG1 level ( J:204685 )

abnormal T-helper 2 physiology ( J:204685 )

• CD4+ T cells and anti-CD3-stimulated splenocytes produce more Th2 cytokines (IL4, IL5 and IL10) compared with wild-type mice

abnormal regulatory T cell physiology ( J:204685 )

• when injected into ovalbumin-immunized wild-type mice, regulatory T cells exacerbate Th2 immune responses in allergic reaction compared with wild-type cells

• however, regulatory T cells exhibit normal suppressive functions and stability

increased regulatory T cell apoptosis ( J:204685 )

• regulatory T cells exhibit increased resistance to activation-induced cell death compared with wild-type cells

Allelic Composition	Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli}/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sor^{tm1(CAG-FOXO1,GFP)Moli} mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:189962 )

N	• mice exhibit normal regulatory T cell differentiation and homeostasis

Allelic Composition	Itch^tm1.1Alta/Itch^tm1.1Alta Gt(ROSA)26Sor^tm1Hjf/Gt(ROSA)26Sor⁺ Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sor^tm1Hjf mutation (5 available); any Gt(ROSA)26Sor mutation (993 available)
Itch^tm1.1Alta mutation (0 available); any Itch mutation (92 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:204685 )

N	• regulatory T cells exhibit normal suppressive functions and stability

increased inflammatory response ( J:204685 )

• severe

Allelic Composition	Becn1^tm1Smoc/Becn1^tm1Smoc Zfp91^tm1Smoc/Zfp91^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Becn1^tm1Smoc mutation (1 available); any Becn1 mutation (36 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Zfp91^tm1Smoc mutation (0 available); any Zfp91 mutation (97 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit higher baseline and maximum glycolytic rates than wild-type T reg cells but similar to either single conditional deletion mice

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression than wild-type T reg cells but similar to either single conditional deletion mice

immune system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit higher baseline and maximum glycolytic rates than wild-type T reg cells but similar to either single conditional deletion mice

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression than wild-type T reg cells but similar to either single conditional deletion mice

Allelic Composition	Foxp3^{tm4(YFP/icre)Ayr}/Y Zfp91^tm1Smoc/Zfp91^tm1Smoc
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Zfp91^tm1Smoc mutation (0 available); any Zfp91 mutation (97 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased susceptibility to induced colitis ( J:306129 )

• mice show increased susceptibility to dextran sodium sulfate (DSS)-induced acute colitis, showing rapid body weight loss, increased clinical scores, exacerbated colon length shortening, increased inflammatory cells into the colon

increased CD8-positive, alpha-beta T cell number ( J:306129 )

• mice show elevated IFN-gamma-producing CD8+ effector T cell proportions in the spleen and mesenteric lymph nodes at 10 weeks of age

decreased regulatory T cell number ( J:306129 )

• mice show low regulatory T (T reg) cell percentages in the spleen and mesenteric lymph nodes at 10 months of age

• however, T cell development and peripheral T cell homeostasis is not affected and T reg cell percentages in the thymus, spleen, and peripheral lymph nodes are normal in 6-week-old mice

abnormal regulatory T cell physiology ( J:306129 )

• adoptive transfer of mutant T reg cells with naive CD45RB^hiCD4+ T cells into RAG-1 deficient mice results in gradual weight loss and a greater frequency of IFN-gamma-producing effector CD4+ T cells that is not seen with wild-type T reg cells

• the fraction of FoxP3+ cells from mice transferred with mutant T reg cells and naive CD45RB^hiCD4+ T cells is decreased compared with those from mice transferred with wild-type T reg cells

• mutant T reg cells transferred into RAG-1 deficient mice cause gradual weight loss, show a more profound loss of Foxp3 expression, and the FoxP3-mainting fraction of mutant T reg cells produce higher amounts of IFN-gamma

• T reg cells exhibit higher baseline and maximum glycolytic rates (increased extracellular acidification rate), indicating hyperglycolysis, however baseline and maximum OXPHOS rates (increased mitochondrial oxygen consumption rate) are not affected

• T regs treated with dichloroacetic acid, which shifts glycolysis towards OXPHOS, show diminished IFN-gamma production

• treatment of stimulated T reg cells with the mTORC1 inhibitor rapamycin lowers the extracellular acidification rate and diminishes IFN-gamma production

• autophagy levels are decreased in T reg cells and autophagy induction after TCR stimulation is severely, but not completely, blocked in T reg cells

increased susceptibility to autoimmune disorder ( J:306129 )

• mice exhibit severe autoimmune symptoms with lymphocytic infiltration in many nonlymphoid organs at 10 months of age

digestive/alimentary system

increased susceptibility to induced colitis ( J:306129 )

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:306129 )

• mice show elevated IFN-gamma-producing CD8+ effector T cell proportions in the spleen and mesenteric lymph nodes at 10 weeks of age

decreased regulatory T cell number ( J:306129 )

• mice show low regulatory T (T reg) cell percentages in the spleen and mesenteric lymph nodes at 10 months of age

• however, T cell development and peripheral T cell homeostasis is not affected and T reg cell percentages in the thymus, spleen, and peripheral lymph nodes are normal in 6-week-old mice

abnormal regulatory T cell physiology ( J:306129 )

• the fraction of FoxP3+ cells from mice transferred with mutant T reg cells and naive CD45RB^hiCD4+ T cells is decreased compared with those from mice transferred with wild-type T reg cells

• T regs treated with dichloroacetic acid, which shifts glycolysis towards OXPHOS, show diminished IFN-gamma production

• treatment of stimulated T reg cells with the mTORC1 inhibitor rapamycin lowers the extracellular acidification rate and diminishes IFN-gamma production

• autophagy levels are decreased in T reg cells and autophagy induction after TCR stimulation is severely, but not completely, blocked in T reg cells

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:306129 )

• mice show increased susceptibility to azoxymethane and DSS-induced colitis-associated cancer, showing higher tumor numbers in the colon than wild-type mice

neoplasm

increased incidence of tumors by chemical induction ( J:306129 )

• mice show increased susceptibility to azoxymethane and DSS-induced colitis-associated cancer, showing higher tumor numbers in the colon than wild-type mice

Allelic Composition	Becn1^tm1Smoc/Becn1^tm1Smoc Foxp3^{tm4(YFP/icre)Ayr}/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit increased extracellular acidification rate indicating higher baseline and maximum glycolytic rates than wild-type T reg cells

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression

immune system

abnormal regulatory T cell physiology ( J:306129 )

• stimulated T reg cells exhibit increased extracellular acidification rate indicating higher baseline and maximum glycolytic rates than wild-type T reg cells

• stimulated T reg cells show much lower Foxp3 expression but higher IFN-gamma expression

Allelic Composition	Cd28^tm1Ltu/Cd28^tm1Ltu Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd28^tm1Ltu mutation (1 available); any Cd28 mutation (52 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Skin inflammation and enlarged lymph nodes and spleen in Cd28^tm1Ltu/Cd28^tm1Ltu Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺ mice

immune system

decreased T cell proliferation ( J:194503 )

• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes

enlarged spleen ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

increased B cell number ( J:194503 )

• in the lymph node

decreased regulatory T cell number ( J:194503 )

• in the thymus but not lymph node or spleens

• in the thymus and periphery of female mice at 3 months

increased activated T cell number ( J:194503 )

• in the lymph nodes and spleen at 2 to 5 months

• with IFN-gamma expression

enlarged lymph nodes ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

increased susceptibility to autoimmune disorder ( J:194503 )

• by 8 to 12 weeks with crusting eyelids, facial hair loss, which progressed to hair loss on the trunk, skin lesions, an ill appearance, ruffled fur, hunching and reduced movements

• however, CD28-sufficient regulatory T cells prevent the autoimmune disease development

increased susceptibility to experimental autoimmune encephalomyelitis ( J:194503 )

• 20 days after immunization, mice treated with rMOG/CFA plus pertussis toxin exhibit increased clinical score and fail to recover compared with wild-type mice

skin inflammation ( J:194503 )

• at 2 to 5 months

integument

skin inflammation ( J:194503 )

• at 2 to 5 months

focal hair loss ( J:194503 )

• facial hair loss, which progressed to hair loss on the trunk by 8 to 12 weeks

ruffled hair ( J:194503 )

• by 8 to 12 weeks

skin lesions ( J:194503 )

• by 8 to 12 weeks

behavior/neurological

hunched posture ( J:194503 )

• by 8 to 12 weeks

decreased locomotor activity ( J:194503 )

• by 8 to 12 weeks

vision/eye

abnormal eyelid morphology ( J:194503 )

• crusting by 8 to 12 weeks

hematopoietic system

decreased T cell proliferation ( J:194503 )

• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes

enlarged spleen ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

increased B cell number ( J:194503 )

• in the lymph node

decreased regulatory T cell number ( J:194503 )

• in the thymus but not lymph node or spleens

• in the thymus and periphery of female mice at 3 months

increased activated T cell number ( J:194503 )

• in the lymph nodes and spleen at 2 to 5 months

• with IFN-gamma expression

growth/size/body

enlarged spleen ( J:194503 )

• at 2 to 5 months with accumulation of activated T cells

cellular

decreased T cell proliferation ( J:194503 )

• reduced proliferation of T regulatory cells in the thymus but not spleen or lymph nodes

Allelic Composition	Nr4a1^tm1Pcn/Nr4a1^tm1Pcn Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Nr4a1^tm1Pcn mutation (0 available); any Nr4a1 mutation (39 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:205658 )

N	• the ratio of YFP+/YFP- regulatory T cells is normal

Allelic Composition	Myd88^tm1.1Medz/Myd88^tm1.1Medz Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Myd88^tm1.1Medz mutation (0 available); any Myd88 mutation (52 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:209373 )

N	• mice mount CD4+ T cell responses in response to immunization with OVA and LPS that are indistinguishable from wild-type mice

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-Il-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-IL-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Allelic Composition	Altre^tm1.1Hbgl/Altre^tm1.1Hbgl Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Altre^tm1.1Hbgl mutation (0 available); any Altre mutation (15 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:342907 )

• reduced survival rate in aged mice

immune system

increased CD8-positive, alpha-beta T cell number ( J:342907 )

• increase in the CD8+ T cell subset in the spleen and liver in aged mice

increased memory T cell number ( J:342907 )

• increase in the percentage of effector memory (CD62L- CD44+) T cells in aged mice

decreased T cell number ( J:342907 )

• decrease in the percentage of naive (CD62L+ CD44-) T cells in aged mice

• difference is more pronounced in the CD8+ T cell compartment

decreased regulatory T cell number ( J:342907 )

• significant decrease in the percentage of T reg cells in the live and slight reduction in the spleen at 14 months of age

• however, no change in T reg percentage is seen in young mice

abnormal regulatory T cell physiology ( J:342907 )

• transfection of T reg cells from aged mice into Rag null mice indicates that the ability of these cells to control T cell proliferation is impaired

• oxygen consumption rate is markedly suppressed in aged but not young T reg cells indicating mitochondrial dysfunction

increased regulatory T cell apoptosis ( J:342907 )

• substantial increase in the liver and smaller increase in the spleen at 14 months of age

increased interleukin-17 secretion ( J:342907 )

• in aged mice

neoplasm

increased liver tumor incidence ( J:342907 )

• at 18 months of age

homeostasis/metabolism

increased circulating alanine transaminase level ( J:342907 )

• at 14 months of age

liver/biliary system

abnormal liver morphology ( J:342907 )

• aged mice (14 months) show more severe hepatic damage compared to age matched controls

increased liver tumor incidence ( J:342907 )

• at 18 months of age

increased susceptibility to diet-induced hepatic steatosis ( J:342907 )

• increase in steatotic hepatocytes, inflammation and fat accumulation in the liver following 8 months on a high fat diet

liver fibrosis ( J:342907 )

• increased at 14 months of age compared to age matched controls

cellular

abnormal mitochondrial morphology ( J:342907 )

• decreased mitochondrial mass in aged T reg cells

decreased mitochondrial size ( J:342907 )

• decreased length of mitochondria in T reg cells from aged mice

increased regulatory T cell apoptosis ( J:342907 )

• substantial increase in the liver and smaller increase in the spleen at 14 months of age

abnormal mitochondrial physiology ( J:342907 )

• decrease in mitochondrial membrane potential in aged but not young T reg cells

• increase in reactive oxygen species in aged but not young T reg cells

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:342907 )

• increase in the CD8+ T cell subset in the spleen and liver in aged mice

increased memory T cell number ( J:342907 )

• increase in the percentage of effector memory (CD62L- CD44+) T cells in aged mice

decreased T cell number ( J:342907 )

• decrease in the percentage of naive (CD62L+ CD44-) T cells in aged mice

• difference is more pronounced in the CD8+ T cell compartment

decreased regulatory T cell number ( J:342907 )

• significant decrease in the percentage of T reg cells in the live and slight reduction in the spleen at 14 months of age

• however, no change in T reg percentage is seen in young mice

abnormal regulatory T cell physiology ( J:342907 )

• transfection of T reg cells from aged mice into Rag null mice indicates that the ability of these cells to control T cell proliferation is impaired

• oxygen consumption rate is markedly suppressed in aged but not young T reg cells indicating mitochondrial dysfunction

increased regulatory T cell apoptosis ( J:342907 )

• substantial increase in the liver and smaller increase in the spleen at 14 months of age

Allelic Composition	Pgd^tm1.1Pse/Pgd^tm1.1Pse Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)
Pgd^tm1.1Pse mutation (0 available); any Pgd mutation (21 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:313817 )

• shorter than normal lifespans

immune system

enlarged spleen ( J:313817 )

ear inflammation ( J:313817 )

• crusting ears

increased T cell number ( J:313817 )

• increased CD4/CD8 positive in the spleen

• increased frequencies of CD44^high increased CD62L^low effector/memory T cells with reduced CD44^low CD62L^high

abnormal CD4-positive helper T cell morphology ( J:313817 )

• increased Th1, Th2, and Th17 responses.

increased IgA level ( J:313817 )

increased IgD level ( J:313817 )

increased IgE level ( J:313817 )

increased IgG2a level ( J:313817 )

increased IgG3 level ( J:313817 )

increased IgM level ( J:313817 )

abnormal regulatory T cell physiology ( J:313817 )

• reduced Tregs suppressive function

increased circulating interferon-gamma level ( J:313817 )

increased circulating interleukin-17 level ( J:313817 )

increased circulating interleukin-4 level ( J:313817 )

increased circulating interleukin-5 level ( J:313817 )

enlarged lymph nodes ( J:313817 )

increased susceptibility to type I hypersensitivity reaction ( J:313817 )

• significant Th2 (allergic) responses in the lung and spleen with increased collagen deposits

increased interferon-gamma secretion ( J:313817 )

• from CD4+ and CD8+ T cells

blepharitis ( J:313817 )

• crusting eyelids

growth/size/body

decreased body size ( J:313817 )

enlarged spleen ( J:313817 )

homeostasis/metabolism

increased circulating interferon-gamma level ( J:313817 )

increased circulating interleukin-17 level ( J:313817 )

increased circulating interleukin-4 level ( J:313817 )

increased circulating interleukin-5 level ( J:313817 )

hearing/vestibular/ear

ear inflammation ( J:313817 )

• crusting ears

vision/eye

blepharitis ( J:313817 )

• crusting eyelids

hematopoietic system

enlarged spleen ( J:313817 )

increased T cell number ( J:313817 )

• increased CD4/CD8 positive in the spleen

• increased frequencies of CD44^high increased CD62L^low effector/memory T cells with reduced CD44^low CD62L^high

abnormal CD4-positive helper T cell morphology ( J:313817 )

• increased Th1, Th2, and Th17 responses.

increased IgA level ( J:313817 )

increased IgD level ( J:313817 )

increased IgE level ( J:313817 )

increased IgG2a level ( J:313817 )

increased IgG3 level ( J:313817 )

increased IgM level ( J:313817 )

abnormal regulatory T cell physiology ( J:313817 )

• reduced Tregs suppressive function

Allelic Composition	Areg^{tm2c(EUCOMM)Hmgu}/Areg^{tm2c(EUCOMM)Hmgu} Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Areg^{tm2c(EUCOMM)Hmgu} mutation (0 available); any Areg mutation (29 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:224904 )

N	• contrary to germ line deletion, mice are born in Mendelian ratios with no gender bias

immune system

immune system phenotype ( J:224904 )

N	• mice exhibit normal frequencies and numbers of B and T cells and activated T cells • unchallenged mice exhibit no heightened inflammatory response and normal T regulator cell suppressor function

increased susceptibility to Orthomyxoviridae infection ( J:224904 )

• flu-infected mice exhibit a greater decrease in average blood oxygen saturation, overall lung tissue damage and decreased surface temperature compared with wild-type mice

• however, flu-infected mice exhibit normal T cell activation and T regulatory cell mediated suppression of anti-vial immunity

homeostasis/metabolism

decreased blood oxygen saturation level ( J:224904 )

• flu-infected mice exhibit a greater decrease in average blood oxygen saturation compared with wild-type mice

• however, mice do not exhibit anemia

decreased body surface temperature ( J:224904 )

• in flu-infected mice

• however, core body temperature is normal

Allelic Composition	Bcl2l11^tm6.1Boui/Bcl2l11^tm6.1Boui Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bcl2l11^tm6.1Boui mutation (1 available); any Bcl2l11 mutation (36 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

increased regulatory T cell number ( J:208318 )

• increased frequency at 2 months that may be due to increased thymic output

• further increase at 6 months as in Bcl2l11^tm1.1Ast homozygotes

immune system

increased regulatory T cell number ( J:208318 )

• increased frequency at 2 months that may be due to increased thymic output

• further increase at 6 months as in Bcl2l11^tm1.1Ast homozygotes

Allelic Composition	Cish^tm1.1Cdon/Cish^tm1.1Cdon Foxp3^{tm4(YFP/icre)Ayr}/Foxp3⁺
Genetic Background	involves: 129S/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cish^tm1.1Cdon mutation (0 available); any Cish mutation (23 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:204829 )

N	• mice exhibit normal asthmatic response

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24