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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		E2f6tm1Lees
targeted mutation 1, Jacqueline A Lees
MGI:3790807
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		E2f6tm1Lees/E2f6tm1Lees involves: 129S4/SvJae * C57BL/6 MGI:3790808
ht2
 		E2f6tm1Lees/E2f6+ involves: 129S4/SvJae * C57BL/6 MGI:3790809
cx3
 		Bmi1tm1Brn/Bmi1tm1Brn
E2f6tm1Lees/E2f6+ 		involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:3790813
cx4
 		Bmi1tm1Brn/Bmi1+
E2f6tm1Lees/E2f6tm1Lees 		involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:3790811
cx5
 		Bmi1tm1Brn/Bmi1tm1Brn
E2f6tm1Lees/E2f6tm1Lees 		involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:3790810
cx6
 		Bmi1tm1Brn/Bmi1+
E2f6tm1Lees/E2f6+ 		involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:3790812


Genotype
MGI:3790808
hm1
Allelic
Composition		 E2f6tm1Lees/E2f6tm1Lees
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f6tm1Lees mutation (0 available); any E2f6 mutation (15 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
thoracic vertebral transformation ( J:134271 )
• transformation of T13 to L1 is seen in 50% of homozygous mice
cervical vertebral transformation ( J:134271 )
• transformation of C5 to C6 is seen in 10% of homozygous mice
lumbar vertebral transformation ( J:134271 )
• transformation of L6 to S1 is seen in 80% of homozygous mice

hematopoietic system
hematopoietic system phenotype ( J:134271 )
N
• unlike mice homozygous for Bmi1tm1Brn no abnormalities in the numbers or distribution of hematopoietic cells is detected

cellular
cellular phenotype ( J:134271 )
N
• unlike mice homozygous for Bmi1tm1Brn MEFs do not display any obvious defects in proliferation , senescence, or ability to re-enter the cell cycle




Genotype
MGI:3790809
ht2
Allelic
Composition		 E2f6tm1Lees/E2f6+
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E2f6tm1Lees mutation (0 available); any E2f6 mutation (15 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
thoracic vertebral transformation ( J:134271 )
• transformation of T13 to L1 is seen in 10% of heterozygous mice
lumbar vertebral transformation ( J:134271 )
• transformation of L6 to S1 is seen in 24% of heterozygous mice




Genotype
MGI:3790813
cx3
Allelic
Composition		 Bmi1tm1Brn/Bmi1tm1Brn
E2f6tm1Lees/E2f6+
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation (2 available); any Bmi1 mutation (31 available)
E2f6tm1Lees mutation (0 available); any E2f6 mutation (15 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
abnormal vertebrae morphology ( J:134271 )
• small extra bone present above C1 in 62% of compound mutant mice
thoracic vertebral transformation ( J:134271 )
• transformation of T7 to T8 and T13 to L1 are seen in 77% and 85% of compound mutant mice, respectively
cervical vertebral transformation ( J:134271 )
• transformation of C1 to C2, C5 to C6, and C7 to T1 are seen in 85%, 23%, and 31% of compound mutant mice, respectively
• partial transformation of C7 to T1 is seen in 77% of compound mutant mice
lumbar vertebral transformation ( J:134271 )
• transformation of L6 to S1 is seen in 92% of compound mutant mice




Genotype
MGI:3790811
cx4
Allelic
Composition		 Bmi1tm1Brn/Bmi1+
E2f6tm1Lees/E2f6tm1Lees
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation (2 available); any Bmi1 mutation (31 available)
E2f6tm1Lees mutation (0 available); any E2f6 mutation (15 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
thoracic vertebral transformation ( J:134271 )
• transformation of T13 to L1 is seen in 77% of compound mutant mice
cervical vertebral transformation ( J:134271 )
• partial or complete transformation of C7 to T1 is seen in 27% and 4% of compound mutant mice, respectively
lumbar vertebral transformation ( J:134271 )
• transformation of L6 to S1 is seen in 92% of compound mutant mice




Genotype
MGI:3790810
cx5
Allelic
Composition		 Bmi1tm1Brn/Bmi1tm1Brn
E2f6tm1Lees/E2f6tm1Lees
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation (2 available); any Bmi1 mutation (31 available)
E2f6tm1Lees mutation (0 available); any E2f6 mutation (15 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:134271 )
• lethality is seen between 3 and 20 weeks of age
postnatal lethality, incomplete penetrance ( J:134271 )
• only 38% of the expected number of mice are present at weaning
prenatal lethality, incomplete penetrance ( J:134271 )
• only 46% of the expected number of embryos are present at E18.5

growth/size/body
postnatal growth retardation ( J:134271 )

skeleton
abnormal vertebrae morphology ( J:134271 )
• small extra bone present above C1 in 38% of double homozygous mice
thoracic vertebral transformation ( J:134271 )
• transformation of T7 to T8 and T13 to L1 are seen in 62% and 100% of double homozygous mice, respectively
cervical vertebral transformation ( J:134271 )
• transformation of C1 to C2, C5 to C6, and C7 to T1 are seen in 92%, 23%, and 8% of double homozygous mice, respectively
• partial transformation of C7 to T1 is seen in 85% of double homozygous mice
lumbar vertebral transformation ( J:134271 )
• transformation of L6 to S1 is seen in 100% of double homozygous mice

hematopoietic system
anemia ( J:134271 )
• severe
abnormal bone marrow cell morphology/development ( J:134271 )
• shift in the distribution of myeloid to B lymphoid cells resulting in an increased percentage of myeloid cells
• within the B cell population, loss of immature B cells is greater than the loss of mature B cells
decreased hematopoietic cell number ( J:134271 )
• decreased number of hematopoietic cells in the thymus, bone marrow and spleen

cellular
abnormal cell cycle ( J:134271 )
• impaired ability of serum-starved MEFs to re-enter the cell cycle
decreased cell proliferation ( J:134271 )
• defect in asynchronous proliferation in MEFs

behavior/neurological
ataxia ( J:134271 )
• similar to that seen in mice homozygous for Bmi1tm1Brn alone at 2 months of age

nervous system
cerebellum hypoplasia ( J:134271 )
• all three layers are affected
• hypoplasia is similar to that in mice homozygous for Bmi1tm1Brn alone

immune system




Genotype
MGI:3790812
cx6
Allelic
Composition		 Bmi1tm1Brn/Bmi1+
E2f6tm1Lees/E2f6+
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmi1tm1Brn mutation (2 available); any Bmi1 mutation (31 available)
E2f6tm1Lees mutation (0 available); any E2f6 mutation (15 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
thoracic vertebral transformation ( J:134271 )
• transformation of T7 to T8 and T13 to L1 are seen in 4% and 50% of double heterozygous mice, respectively
cervical vertebral transformation ( J:134271 )
• partial or complete transformation of C7 to T1 is seen in 42% and 8% of double heterozygous mice, respectively
• transformation of C5 to C6 is seen in 4% of double heterozygous mice
lumbar vertebral transformation ( J:134271 )
• transformation of L6 to S1 is seen in 73% of double heterozygous mice




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory