Phenotypes associated with this allele

• Allele Symbol: Wnt7b^tm2.1Amc
• Allele Name: targeted mutation 2.1, Andrew P McMahon
• Allele ID: MGI:3793280
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wnt7b^tm2.1Amc/Wnt7b^tm2.1Amc	involves: 129X1/SvJ	MGI:3793500
cn2	Gt(ROSA)26Sor^tm1Sor/? Wnt7b^tm2Amc/Wnt7b^tm2.1Amc Tg(Hoxb7-cre)13Amc/0	involves: 129S4/SvJaeSor * 129X1/SvJ * C57BL/6	MGI:3829882
cn3	Wnt7b^tm2Amc/Wnt7b^tm2.1Amc Tg(Hoxb7-cre)13Amc/0	involves: 129X1/SvJ * C57BL/6	MGI:3829881
cn4	Wnt7b^tm2Amc/Wnt7b^tm2.1Amc Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:3793499

Genotype
MGI:3793500

hm1

• Allelic Composition: Wnt7b^tm2.1Amc/Wnt7b^tm2.1Amc
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:134483 )

• mice exhibit placental lethality

Genotype
MGI:3829882

cn2

• Allelic Composition: Gt(ROSA)26Sor^tm1Sor/?; Wnt7b^tm2Amc/Wnt7b^tm2.1Amc; Tg(Hoxb7-cre)13Amc/0
• Genetic Background: involves: 129S4/SvJaeSor * 129X1/SvJ * C57BL/6

renal/urinary system

dilated kidney collecting duct ( J:142685 )

• at E15.5 the collecting ducts are dilated and branch points are less evident

Genotype
MGI:3829881

cn3

• Allelic Composition: Wnt7b^tm2Amc/Wnt7b^tm2.1Amc; Tg(Hoxb7-cre)13Amc/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

mortality/aging

postnatal lethality, complete penetrance ( J:142685 )

• survive to P11 - P12

renal/urinary system

decreased urine osmolality ( J:142685 )

• at P10, urine osmolality is 56% that of controls

abnormal kidney morphology ( J:142685 )

• at P11 - P12 kidneys are grossly abnormal

absent kidney medulla ( J:142685 )

• absent at P10

kidney failure ( J:142685 )

• by P11 - P12 kidneys are physiologically incompetent

homeostasis/metabolism

decreased urine osmolality ( J:142685 )

• at P10, urine osmolality is 56% that of controls

Genotype
MGI:3793499

cn4

• Allelic Composition: Wnt7b^tm2Amc/Wnt7b^tm2.1Amc; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

mortality/aging

neonatal lethality, complete penetrance ( J:134483 )

• mice die within hours of birth

respiratory system

lung hemorrhage ( J:134483 )

abnormal lung development ( J:134483 )

• lung epithelial and mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice

• however, normal cell differentiation and patterning are preserved

decreased mesenchymal cell proliferation involved in lung development ( J:134483 )

• mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice

thin lung-associated mesenchyme ( J:134483 )

• the lung mesenchyme is thinner than in wild-type mice

abnormal right lung middle lobe morphology ( J:134483 )

• the medial lobe bronchus of the right lung emanates from the cephalic lobe bronchus, unlike in wild-type mice

• however, the correct number of lobes is formed

small lung ( J:134483 )

• at E12.5

decreased lung weight ( J:134483 )

• lung weight is 50% of wild-type at E18.5 and 25% of wild-type at E14.5

atelectasis ( J:134483 )

• lungs are poorly inflated

abnormal tracheal cartilage morphology ( J:134483 )

• cartilaginous rings are incomplete

homeostasis/metabolism

cyanosis ( J:134483 )

• after birth mice become cyanotic despite chest wall movements

renal/urinary system

increased kidney apoptosis ( J:142685 )

• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells

abnormal kidney cell proliferation ( J:142685 )

• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis

decreased kidney cell proliferation ( J:142685 )

• cell proliferation in the loop of Henle is significantly reduced

abnormal kidney morphology ( J:142685 )

• renal corpuscles abut the renal pelvis

• despite the absence of the medulla, kidneys are similar in size to controls

abnormal kidney collecting duct epithelium morphology ( J:142685 )

• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis

• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells

abnormal kidney medulla development ( J:142685 )

• at E15.5 and E16.5, the nascent medulla is absent indicating a failure to initiate medulla formation

absent kidney medulla ( J:142685 )

• absent at E18.5

truncated loop of Henle ( J:142685 )

• at E18.5 the loop of Henle is truncated resembling morphology at an earlier stage prior to loop elongation

• cell proliferation in the loop of Henle is significantly reduced

hydroureter ( J:142685 )

• at E18.5, less than a third of mice show hydroureter like swelling of the pelvic region

skeleton

abnormal tracheal cartilage morphology ( J:134483 )

• cartilaginous rings are incomplete

cardiovascular system

lung hemorrhage ( J:134483 )

cellular

increased kidney apoptosis ( J:142685 )

• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells

abnormal kidney cell proliferation ( J:142685 )

• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis

decreased kidney cell proliferation ( J:142685 )

• cell proliferation in the loop of Henle is significantly reduced

decreased mesenchymal cell proliferation involved in lung development ( J:134483 )

• mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice