About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ebi3Gt716Lex
gene trap 716, Lexicon Genetics
MGI:3793965
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ebi3Gt716Lex/Ebi3Gt716Lex involves: 129S5/SvEvBrd * C57BL/6 MGI:6442003
cx2
Ebi3Gt716Lex/Ebi3Gt716Lex
Tg(DO11.10)10Dlo/0
involves: 129S5/SvEvBrd * BALB/c * C3H * C57BL/6 MGI:3795472


Genotype
MGI:6442003
hm1
Allelic
Composition
Ebi3Gt716Lex/Ebi3Gt716Lex
Genetic
Background
involves: 129S5/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ebi3Gt716Lex mutation (0 available); any Ebi3 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice infected intracranially with mouse hepatitis virus strain J2.2v-1 (JHMV) show increased susceptibility to JHMV-induced death, with approximately 70% dying by 12 days post infection (dpi) compared to about 30% of wild-type mice

immune system
• JHMV-infected mice show reduced numbers of CD4+ T cell within the brain at 5 dpi but levels return to normal at 7 and 12 dpi
• JHMV-infected mice show reduced numbers of virus-specific CD4+ T cells within the CNS at 7 dpi but increased numbers by 12 dpi
• JHMV-infected mice show decreased numbers of virus-specific CD4+ T cells isolated from spleens
• JHMV-infected mice show reduced numbers of CD8+ T cells within the brains at 5 dpi but numbers are increased by 7 dpi and this increase in sustained to 12 dpi
• JHMV-infected mice show increased numbers of virus-specific CD8+ T cells within the CNS at 7 and 12 dpi
• however, numbers of virus-specific CD8+ T cells present in the spleen are similar to wild-type
• macrophage infiltration is elevated in JHMV-infected mice at 7 and 12 dpi
• CD4+ and CD8+ T cells show enhanced activation (CD62L-CD25+) states within the central nervous system (CNS)
• JHMV virus-specific CD4+ T cells exhibit an approximate 2-fold increase in IFN-gamma production and CD8+ T cells show a more than 2.5-fold increase in IFN-gamma levels in response to peptide stimulation compared to wild-type mice
• JHMV virus-specific T cells show a reduction in 1L-10 production
• JHMV-infected mice show increased thickening of the meninges and increased perivascular cuffing indicating enhanced neuroinflammation
• mice exhibit increased sensitivity to mouse hepatitis virus strain J2.2v-1 (JHMV) infection, showing elevated titers by 7 days post infection (dpi) which are reduced to below detection by 12 dpi indicating ability to control viral replication but showing increased disease severity with enhanced leukocyte infiltration and T cell activation and increased IFN-gamma secretion
• mice infected intracranially with mouse hepatitis virus strain J2.2v-1 (JHMV) show increased susceptibility to JHMV-induced death, with approximately 70% dying by 12 days post infection (dpi) compared to about 30% of wild-type mice

hematopoietic system
• JHMV-infected mice show reduced numbers of CD4+ T cell within the brain at 5 dpi but levels return to normal at 7 and 12 dpi
• JHMV-infected mice show reduced numbers of virus-specific CD4+ T cells within the CNS at 7 dpi but increased numbers by 12 dpi
• JHMV-infected mice show decreased numbers of virus-specific CD4+ T cells isolated from spleens
• JHMV-infected mice show reduced numbers of CD8+ T cells within the brains at 5 dpi but numbers are increased by 7 dpi and this increase in sustained to 12 dpi
• JHMV-infected mice show increased numbers of virus-specific CD8+ T cells within the CNS at 7 and 12 dpi
• however, numbers of virus-specific CD8+ T cells present in the spleen are similar to wild-type
• macrophage infiltration is elevated in JHMV-infected mice at 7 and 12 dpi
• CD4+ and CD8+ T cells show enhanced activation (CD62L-CD25+) states within the central nervous system (CNS)

nervous system
• JHMV-infected mice show increased thickening of the meninges and increased perivascular cuffing indicating enhanced neuroinflammation
• JHMV-infected mice show increased astrogliosis




Genotype
MGI:3795472
cx2
Allelic
Composition
Ebi3Gt716Lex/Ebi3Gt716Lex
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129S5/SvEvBrd * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ebi3Gt716Lex mutation (0 available); any Ebi3 mutation (12 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• following anti-CD3 antibody stimulation, differentiation into Th17 cells is increased compared to in wild-type mice
• following ovalbumin stimulation or infection with Listeriosis monocytogenes, Il-22 plasma levels are increased compared to in wild-type mice
• following ovalbumin stimulation or infection with Listeriosis monocytogenes
• following anti-CD3 antibody stimulation
• mice are resistant to infection with Listeriosis monocytogenes with a 45-fold less bacteria load in the spleen and a 150-fold less bacterial load in the liver after re-challenge compared to in similarly treated wild-type mice

homeostasis/metabolism
• following ovalbumin stimulation or infection with Listeriosis monocytogenes, Il-22 plasma levels are increased compared to in wild-type mice
• following ovalbumin stimulation or infection with Listeriosis monocytogenes

hematopoietic system
• following anti-CD3 antibody stimulation, differentiation into Th17 cells is increased compared to in wild-type mice





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory