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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Akt1tm1Pnt
targeted mutation 1, Philip N Tsichlis
MGI:3794787
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Tg(Lck-cre)1Cwi/0 		involves: 129P2/OlaHsd MGI:3803971
cn2
 		Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0 		involves: 129P2/OlaHsd MGI:3803972
cn3
 		Akt1tm1Pnt/Akt1tm1Pnt
Tg(Lck-cre)1Cwi/0 		Not Specified MGI:3803966
cn4
 		Akt1tm1Pnt/Akt1tm1Pnt
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0 		Not Specified MGI:3803970


Genotype
MGI:3803971
cn1
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Akt2tm1.1Mbb mutation (2 available); any Akt2 mutation (53 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased T cell apoptosis ( J:135903 )
• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 15-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 56.2% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 25% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are partially blocked at the DN3 to DN4 stage and the DN4 to the DP stage

hematopoietic system
increased T cell apoptosis ( J:135903 )
• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 15-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 56.2% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 25% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are partially blocked at the DN3 to DN4 stage and the DN4 to the DP stage

cellular
increased T cell apoptosis ( J:135903 )
• an increased percentage of double positive thymocytes are apoptotic (15.9% compared to 2.6%)

endocrine/exocrine glands
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 15-fold




Genotype
MGI:3803972
cn2
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Akt2tm1.1Mbb/Akt2tm1.1Mbb
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Akt2tm1.1Mbb mutation (2 available); any Akt2 mutation (53 available)
Akt3tm1Mbb mutation (0 available); any Akt3 mutation (42 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
increased T cell apoptosis ( J:135903 )
• double negative thymocytes of all stages have higher rates of apoptosis
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 20-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 85.4% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 1.8% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are almost completely blocked at the DN4 to DP transition

immune system
increased T cell apoptosis ( J:135903 )
• double negative thymocytes of all stages have higher rates of apoptosis
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 20-fold
increased double-negative T cell number ( J:135903 )
• the percentage of double negative T cells in the thymus is 85.4% compared to 2.7% in wild-type mice
• there is a significant reduction in proliferating DN4 cells suggesting a partial development block in transitioning to the DP stage
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 1.8% compared to 84.6% in wild-type mice
• the actual number of DP T cells is 50-fold less than is found in wild-type thymuses
arrested T cell differentiation ( J:135903 )
• developing thymocytes are almost completely blocked at the DN4 to DP transition

cellular
increased T cell apoptosis ( J:135903 )
• double negative thymocytes of all stages have higher rates of apoptosis

endocrine/exocrine glands
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 20-fold




Genotype
MGI:3803966
cn3
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Tg(Lck-cre)1Cwi/0
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:135903 )
• no abnormalities in T cell development are observed




Genotype
MGI:3803970
cn4
Allelic
Composition		 Akt1tm1Pnt/Akt1tm1Pnt
Akt3tm1Mbb/Akt3tm1Mbb
Tg(Lck-cre)1Cwi/0
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Pnt mutation (0 available); any Akt1 mutation (34 available)
Akt3tm1Mbb mutation (0 available); any Akt3 mutation (42 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 2.5 fold
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 78.2% compared to 84.6% in wild-type mice

immune system
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 2.5 fold
decreased double-positive T cell number ( J:135903 )
• the percentage of double positive T cells in the thymus is reduced to 78.2% compared to 84.6% in wild-type mice

endocrine/exocrine glands
thymus hypoplasia ( J:135903 )
• thymus cellularity is reduced by about 2.5 fold




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory