Analysis Tools
behavior/neurological
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• mice exhibit normal activity in locomotion tests, heat and punctate mechanical nociception, and fear response
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• inability to right following scruffing
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• following scruffing, mice assume a rigid posture
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• following scruffing, tails exhibit a waxy flexibility that allow tail to retain a manually set position
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• however, capsaicin treatment abrogates hypersensitivity to cold
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• following scruffing as early as 1 week of age, mice exhibit arrest of all movement, rigid posture, transient apnea, absent righting response, and tail waxy flexibility unlike wild-type mice
• scruffing phenotype persists throughout the life of the mouse without habituation
• however, mice return to normal behavior after 1 to 5 minutes and auditory or mechanical stimuli promotes recovery
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nervous system
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• small dorsal root ganglion neurons with long-duration action potentials are hyper-excitable compared with wild-type cells
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• during the immobile period after scruffing
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• whole cell current is enhanced compared to in wild-type mice
• the depolarizing current required to elicit an action potential is decreased compared to in wild-type mice
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cardiovascular system
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• during the immobile period after scruffing
• however, treatment with atropine prevents decrease in heart rate during immobility
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• during the immobile period after scruffing
• however, treatment with atropine prevents normalizes cardiac rhythm during immobility
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respiratory system
