Analysis Tools
cellular
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• decrease number of elongating/elongated spermatids occurs in the testis of 5 month old males
• total number of elongated spermatid in 9 month old testis is significantly reduced but some are still present
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• the percentage of sperm with motility is half that of controls at 2 months of age whether progressive or total motility is measured
• sperm motility is almost completely absent by 5 months of age
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growth/size/body
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• mice have significantly lower weight than controls at 2 months of age
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reproductive system
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• the percentage of sperm with motility is half that of controls at 2 months of age whether progressive or total motility is measured
• sperm motility is almost completely absent by 5 months of age
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• caudal epididymal sperm counts are reduced and worsen with age
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• male germ cells are often found at inappropriate depth in the seminiferous epithelium or adjacent to germ cells at different stages of spermatogenesis
• examples of this include round spermatid in stage X tubules or diplotene spermatocytes juxtapositioned with round spermatids within the same tubule
• this disorganization increases with age of the male
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• decrease number of elongating/elongated spermatids occurs in the testis of 5 month old males
• total number of elongated spermatid in 9 month old testis is significantly reduced but some are still present
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• increased numbers of elongated spermatid are retained in the seminiferous epithelium of 5 month old males
• stage VIII and stage IX spermatid are often not orientated in the proper direction with acrosomes pointed away from the basement membrane
• spermiation failure becomes progressively more abundant with age
• failure of apical ectoplasmic specialization resolution between spermatids and Sertoli cells is observed at high resolution
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• increase vacuolation occurs in the seminiferous epithelium of 5 month old males that is suggestive of germ cell loss
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• cytoplasm of Sertoli cells in 5 month old males is vacuolated and has a lacy appearance
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• failure of apical ectoplasmic specialization resolution between spermatids and Sertoli cells is observed at high resolution
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• testis have lower weight both in absolute and relative to body weight measurements compared to control males starting at 2 months of age
• testis weight is 82% of controls at 2 months of age and 59% by 9 months of age
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• 5 month old males have a massive increase in the number of round spermatid found in the lumen of the epididymis
• spermatid and spermatocytes are visible within the epididymidal lumen by 1 month of age and maintained until at least 9 months of age
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• litters sired by mutant males are of smaller size and litter sizes decrease with age of the mutant father
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• no males are capable of siring litters after 150 days of age
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• reduced fertility among male mice that gets worse with age
• 3 of 8 young males are sterile, 2 produce only one litter, 1 produces two litters and 2 produce 4 litters
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endocrine/exocrine glands
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• increase vacuolation occurs in the seminiferous epithelium of 5 month old males that is suggestive of germ cell loss
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• cytoplasm of Sertoli cells in 5 month old males is vacuolated and has a lacy appearance
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• failure of apical ectoplasmic specialization resolution between spermatids and Sertoli cells is observed at high resolution
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• testis have lower weight both in absolute and relative to body weight measurements compared to control males starting at 2 months of age
• testis weight is 82% of controls at 2 months of age and 59% by 9 months of age
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