Phenotypes associated with this allele

• Allele Symbol: Cdca8^tm1Tatn
• Allele Name: targeted mutation 1, Tatsutoshi Nakahata
• Allele ID: MGI:3795253
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cdca8^tm1Tatn/Cdca8^tm1Tatn	involves: C57BL/6 * CBA	MGI:3798036
cx2	Cdca8^tm1Tatn/Cdca8^tm1Tatn Trp53^tm1Brd/Trp53^tm1Brd	involves: 129S7/SvEvBrd * C57BL/6 * CBA	MGI:3798037

Genotype
MGI:3798036

hm1

• Allelic Composition: Cdca8^tm1Tatn/Cdca8^tm1Tatn
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:135988 )

• at E3.5 embryos are present but fail to form a fully expanded blastocyst

• by E5.5 embryos are disorganized, degenerate, and in advanced stage of resorption

embryo

failure of blastocyst to hatch from the zona pellucida ( J:135988 )

• cultured E3.5 blastocysts fail to hatch from the zona pellucida

cellular

abnormal cell nucleus morphology ( J:135988 )

• at E3.5 abnormal embryos show micronuclei formation, irregular nuclei, and variable nuclear size

increased cell nucleus count ( J:135988 )

• at E3.5 abnormal embryos show multinucleation

abnormal mitosis ( J:135988 )

• at E3.5 abnormal embryos show diffuse and patchy tubulin staining and lack intercellular bodies indicating failure to reach telophase/cytokinesis

increased apoptosis ( J:135988 )

• by E5.5

Genotype
MGI:3798037

cx2

• Allelic Composition: Cdca8^tm1Tatn/Cdca8^tm1Tatn; Trp53^tm1Brd/Trp53^tm1Brd
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * CBA

mortality/aging

embryonic lethality, complete penetrance ( J:135988 )

• no embryos are found at E7.5