Phenotypes associated with this allele

• Allele Symbol: Tg(Tg-cre)1Soff
• Allele Name: transgene insertion 1, Stefan Offermanns
• Allele ID: MGI:3796561
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gna11^tm1Soff/Gna11^tm1Soff Gnaq^tm2Soff/Gnaq^tm2Soff Tg(Tg-cre)1Soff/0	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6	MGI:3796562

Genotype
MGI:3796562

cn1

• Allelic Composition: Gna11^tm1Soff/Gna11^tm1Soff; Gnaq^tm2Soff/Gnaq^tm2Soff; Tg(Tg-cre)1Soff/0
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:127484 )

N • as mutants age, no significant differences in thyroid size or weight is observed relative to wild-type

abnormal thyroid gland morphology ( J:127484 )

• at 6 months of age, histology is altered; follicular structure is disturbed with few normal follicles remaining

• no increase in thyroid gland weight is observed in 6-8 week-old mice treated with TSH (thyroid stimulating hormone) for 1 week, whereas wild-type mice show a 100% increase in thyroid weight; glands in mutants actually decrease in weight due to slight decrease in follicular lumen size

• no increase in thyrocyte number is observed after TSH treatment in contrast to increase seen in wild-type; thryocytes show hypertrophic response to TSH treatment

• after TSH treatment, colloid area is reduced unlike wild-type thyroids

• mutant thyroids show no response to goitrogenic diet, whereas wild-type mice readily develop hyperplastic goiters

abnormal thyroid follicular cell morphology ( J:127484 )

• cells are often enlarged, columnar and have large nuclei at 6 months of age

abnormal thyroid gland physiology ( J:127484 )

• TSH-induced thyroid hormone release is almost entirely abrogated in mutants

• impaired pinocytic uptake of colloid and reduction in pinocytic vesicle formation in thyroid follicular epithelium upon TSH stimulation is observed in mutants versus wild-type

decreased activity of thyroid gland ( J:127484 )

• after 6 months of age, about half of animals display overt hypothyroidism

homeostasis/metabolism

decreased circulating thyroxine level ( J:127484 )

• at 6 months, mice show low T4 levels compared to wild-type

increased circulating thyroid-stimulating hormone level ( J:127484 )

• beginning at 2 months of age, TSH (thyroid stimulating hormone) plasma levels start to rise and are significantly higher than wild-type at 6 months of age