Analysis Tools|
Allele Symbol Allele Name Allele ID |
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde targeted mutation 1, Michael D Ehlers MGI:3796847 |
||||||||||||||||
| Summary |
3 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• capsaicin infusion after focal induction of Trpv1 expression in subsets of striatal neurons by injection of a lentiviral-cre vector recapitulates contralateral circling behavior at one week post-injection; circling subsides within 15 minutes of capsaicin application
• ipsilateral circling is not observed after capsaicin infusion
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• cheek injection of capsaicin does not evoke pain behavior (forepaw facial wiping), in contrast to robust facial wiping observed in treated wild-type
|
|
• mice have impaired nociceptive responses, showing increased latencies in tail-immersion and hot plate paradigms
|
|
• robust itch behavior is observed after cheek injection of capsaicin compared to little hindpaw scratching seen in treated wild-type
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• capsaicin infusion into striatum does not affect exploratory or motor behavior in Cre-activated mutants or controls
|
|
• infusion of capsaicin into striatum of results in stereotyped contralateral circling behavior within 5 minutes of delivery, while control mice without activation of Trpv1 show no effect
|
|
• infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
• at doses sufficient to induce circling, no cell death is observed
|
|
• in layer 5 cortical slices, capsaicin induces action potentials while cells from Cre-negative animals show no response
• neurons in slices show dose-dependent depolarizing currents in response to capsaicin applied to the bath
• capsaicin applied acutely to single cells causes reproducible bursts of action potentials with increases in firing rate; frequency of firing is dependent on duration and concentration of capsaicin application
|
|
• infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
• at doses sufficient to induce circling, no cell death is observed
|
|
• infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
• at doses sufficient to induce circling, no cell death is observed
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 11/19/2024 MGI 6.24 |
|
|
|
||
