Phenotypes associated with this allele

• Allele Symbol: Flrt3^tm1Rob
• Allele Name: targeted mutation 1, Elizabeth J Robertson
• Allele ID: MGI:3797077
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Flrt3^tm1Rob/Flrt3^tm1Rob	involves: 129S/SvEv	MGI:4950440
hm2	Flrt3^tm1Rob/Flrt3^tm1Rob	involves: 129S/SvEv * C57BL/6J	MGI:3805960

Genotype
MGI:4950440

hm1

• Allelic Composition: Flrt3^tm1Rob/Flrt3^tm1Rob
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

preweaning lethality, incomplete penetrance ( J:171515 )

• fewer than expected mice survive to weaning

• however, over-expression of Flrt2 partially rescues lethality

cellular

impaired basement membrane formation ( J:171515 )

• in embryoid bodies

• however, however, over-expression of Flrt2 partially rescues basement membrane formation

Genotype
MGI:3805960

hm2

• Allelic Composition: Flrt3^tm1Rob/Flrt3^tm1Rob
• Genetic Background: involves: 129S/SvEv * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:136719 )

• at E13.5, >13% of expected 25% homozygous null embryos are recovered

• <3% of Flrt3-deficient embryos complete development and are viable and fertile as adults

embryo

abnormal endoderm development ( J:136719 )

• proliferation and migration of definitive endoderm is compromised in mutants; at E7.5, definitive endoderm cells are underrepresented and disorganized

rostral body truncation ( J:136719 )

• about one-third of mutants display pronounced anterior truncations

abnormal embryonic tissue morphology ( J:136719 )

• development of foregut pocket appears to be disrupted

abnormal embryonic neuroepithelium morphology ( J:136719 )

• severely affected embryos with anterior truncations show loss of neurectoderm

abnormal neural tube closure ( J:136719 )

• defective fusion of the anterior neural region

incomplete rostral neuropore closure ( J:136719 )

• by E8.5, about one third of mutants show abnormal neuroepithelium morphogenesis and headfold fusion defects

cardiovascular system

abnormal heart development ( J:136719 )

• proepicardial cells which give rise to epicardium and coronary vasculature do not migrate toward the midline and intercalate as in wild-type embryos

• cardiac progenitors form a heart tube that undergoes normal chamber formation

cardia bifida ( J:136719 )

• about 30% of E8.5 embryos display a pronounced ventral closure defect always associated with cardia bifida

nervous system

abnormal embryonic neuroepithelium morphology ( J:136719 )

• severely affected embryos with anterior truncations show loss of neurectoderm

abnormal neural tube closure ( J:136719 )

• defective fusion of the anterior neural region

incomplete rostral neuropore closure ( J:136719 )

• by E8.5, about one third of mutants show abnormal neuroepithelium morphogenesis and headfold fusion defects

growth/size/body

failure of ventral body wall closure ( J:136719 )

• development of foregut pocket appears to be disrupted, contributing to failure of ventral closure