Analysis Tools
nervous system
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• in culture, primary microglia isolated from newborn pups exhibit an increased number of TUNEL+ apoptotic cells relative to wild-type microglia
• protein level of cleaved Caspase-3 (c-Casp3) is increased in microglia cell lysates, whereas protein levels of pro-Caspase3 (the inactive precursor to c-Casp3) and Bcl-2 (an anti-apoptotic protein) are decreased, suggesting accelerated microglial apoptosis
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• decreased cell viability (survival) in microglia, as assessed by MTT assay
• decreased microglia cell proliferation, as shown by BrdU incorporation assay
• in culture, primary microglia isolated from newborn pups exhibit an increased number of TUNEL+ apoptotic cells relative to wild-type microglia
• microglia show accelerated degradation of beta-catenin and severe downregulation of Wnt/beta-catenin signaling; beta-catenin levels, microglial cell survival and proliferation can be partially restored by Wnt3a or LiCl or TDZD-8 treatment
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• following neuronal injury induced by kainic acid treatment, mice exhibit decreased microgliosis with significantly reduced morphological changes and number of activated microglia relative to wild-type controls, esp. in the hippocampus
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cellular
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• FACS analysis of microglia revealed an accumulation of cells in the G0/G1 phase of the cell cycle and a decreased percentage of cells in the S phase, indicating cell cycle arrest at the G1/S checkpoint
• mRNA and protein levels of Cyclin D1 and c-Myc are significantly decreased relative to those in wild-type microglia
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• in culture, primary microglia isolated from newborn pups exhibit an increased number of TUNEL+ apoptotic cells relative to wild-type microglia
• protein level of cleaved Caspase-3 (c-Casp3) is increased in microglia cell lysates, whereas protein levels of pro-Caspase3 (the inactive precursor to c-Casp3) and Bcl-2 (an anti-apoptotic protein) are decreased, suggesting accelerated microglial apoptosis
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immune system
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• decreased cell viability (survival) in microglia, as assessed by MTT assay
• decreased microglia cell proliferation, as shown by BrdU incorporation assay
• in culture, primary microglia isolated from newborn pups exhibit an increased number of TUNEL+ apoptotic cells relative to wild-type microglia
• microglia show accelerated degradation of beta-catenin and severe downregulation of Wnt/beta-catenin signaling; beta-catenin levels, microglial cell survival and proliferation can be partially restored by Wnt3a or LiCl or TDZD-8 treatment
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• following neuronal injury induced by kainic acid treatment, mice exhibit decreased microgliosis with significantly reduced morphological changes and number of activated microglia relative to wild-type controls, esp. in the hippocampus
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hematopoietic system
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• decreased cell viability (survival) in microglia, as assessed by MTT assay
• decreased microglia cell proliferation, as shown by BrdU incorporation assay
• in culture, primary microglia isolated from newborn pups exhibit an increased number of TUNEL+ apoptotic cells relative to wild-type microglia
• microglia show accelerated degradation of beta-catenin and severe downregulation of Wnt/beta-catenin signaling; beta-catenin levels, microglial cell survival and proliferation can be partially restored by Wnt3a or LiCl or TDZD-8 treatment
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• following neuronal injury induced by kainic acid treatment, mice exhibit decreased microgliosis with significantly reduced morphological changes and number of activated microglia relative to wild-type controls, esp. in the hippocampus
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