Phenotypes associated with this allele

• Allele Symbol: Gria1^tm2Rsp
• Allele Name: targeted mutation 2, Rolf Sprengel
• Allele ID: MGI:3798452
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gria1^tm2Rsp/Gria1^tm2Rsp	involves: 129S1/Sv * 129X1/SvJ	MGI:5513851
cn2	Gria1^tm2Rsp/Gria1^tm2Rsp Tg(Pvalb-cre)1Mony/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3804917
cn3	Gria1^tm2Rsp/Gria1^tm2Rsp Tg(Slc6a3-icre)9075Gsc/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:3835857

Genotype
MGI:5513851

cn1

• Allelic Composition: Gria1^tm2Rsp/Gria1^tm2Rsp
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal miniature excitatory postsynaptic currents ( J:197912 )

• reduced amplitude with faster decay in cre-infected neurons

Genotype
MGI:3804917

cn2

• Allelic Composition: Gria1^tm2Rsp/Gria1^tm2Rsp; Tg(Pvalb-cre)1Mony/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:136763 )

N • mutants show comparable behavior to wild-type in tests for object recognition memory and spatial change

abnormal spatial working memory ( J:136763 )

• moderate impairment is exhibited in rewarded alternation task compared to wild-type

• spatial reference memory is unimpaired in Y maze paradigm

nervous system

abnormal nervous system electrophysiology ( J:136763 )

• power of gamma oscillations in CA3 region hippocampal slices induced by kainate is reduced compared to wild-type

impaired ability to fire action potentials ( J:136763 )

• stronger stimuli are needed to evoke action potential (AP) firing from fast-spiking cells; average probability of firing is close to 1 in wild-type cells vs 0.7 in mutant cells

abnormal excitatory postsynaptic potential ( J:136763 )

• compound EPSPs in interneurons are significantly smaller in slices from mutants than in slices from wild-type; EPSPs also have longer decay times

reduced AMPA-mediated synaptic currents ( J:136763 )

• no reduction in AMPA current is seen at P14, but AMPA-mediated currents are significantly reduced at P42, comparable with the reduction detected in Gria4 mutants at P42

Genotype
MGI:3835857

cn3

• Allelic Composition: Gria1^tm2Rsp/Gria1^tm2Rsp; Tg(Slc6a3-icre)9075Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

behavior/neurological

enhanced conditioned place preference behavior ( J:139693 )

• mice do not lose the preference for an environment where cocaine was previously administered (i.e. conditioned place preference) even after 16 days have passed since the last cocaine dosing

nervous system

abnormal glutamate-mediated receptor currents ( J:139693 )

• there is no increase in the ratio of AMPA-/NMDA- mediated receptor currents for the ventral tegmental area isolated from mice treated with cocaine as occurs in wild-type controls

• cocaine-induced synaptic strengthening in the ventral tegmental area does not occur in these mice