Analysis Tools
homeostasis/metabolism
|
• when fed a 0.2% cholesterol diet, modestly but significantly, higher amounts of hepatic free cholesterol and phospholipids are found, compared to wild-type
• no difference in cholesteryl ester or triglyceride concentrations are observed on this diet
|
|
• when fed a 0.015% cholesterol diet, plasma total cholesterol is increased by 43% and plasma free cholesterol is elevated 41% relative to wild-type; on a 0.2% cholesterol diet, plasma total cholesterol and free cholesterol are significantly higher than in wild-type mice
|
liver/biliary system
|
• on a 0.015% cholesterol diet, gallbladder cholesterol is increased 13-fold compared to wild-type, whereas phospholipid and bile acid concentrations are maintained
• on a 0.2% cholesterol diet, cholesterol increases in the gallbladder, but levels are 5-fold lower than wild-type
• molar ratios of biliary cholesterol are 14-fold lower when fed a 0.015% cholesterol diet, and 5-fold lower on a 0.2% cholesterol diet compared to wild-type mice
• biliary cholesterol excretion is restored by treatment with ezetimide for 4 days; bile from treated mice has a 10-fold higher cholesterol concentration compared to vehicle-treated transgenic mice, but molar ratio of biliary cholesterol in treated mutants is similar to levels in treated wild-type
|
