Analysis Tools
mortality/aging
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• most die before 5 weeks of age
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growth/size/body
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• visibly smaller at birth
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• lower average weights at 2, 3, and 4 weeks of age
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weight loss
(
J:137415
)
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• during the week before death mice lose weight
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behavior/neurological
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• seen in about 40% of mice
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nervous system
hydrocephaly
(
J:137415
)
|
• the arbor vitae is severely disorganized and hypocellular
|
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• neuronal loss is seen in the major commissures
• the cerebellar commissures are severely disorganized and hypocellular
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• in 2 and 4 week old mice, the corpus callosum is hypocellular and disorganized
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• hypocellular
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• the arbor vitae and cerebellar commissures are severely disorganized and hypocellular
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hematopoietic system
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• disproportionate reduction in double positive thymocytes and accumulation of double negative and singly positive T cells
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• weight is reduced even when smaller body weight is factored in
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• decrease in the total number of B cell with the greatest reduction in the proportion of pre-B cells
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• about 2% of erythrocytes have micronuclei compared to less than 0.1% in controls
• however, hemoglobin levels appear normal
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• contain more extensively segmented nuclei
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• developmental block at the earliest stage of B cell development indicated by an increase in the proportion of pre-pro-B cells
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• virtually absent from the peritoneal cavity
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• virtually absent from the peritoneal cavity
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• greatest reductions are seen in the proportion of B220+ CD25+ pre-B cells
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• red pulp surrounding lymphoid follicles is disorganized
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small spleen
(
J:137415
)
|
• significantly smaller even when reduction in body size is factored in
|
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• total cellularity is reduced to 12 +/- 7.1% of controls at 16 - 25 days of age
|
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• lymphoid follicles are not clearly defined
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• the proportion of B cells is reduced and the proportion of T cells is increased
• a disproportionate reduction in immature B cells is seen especially for T1 type cells accompanied by a modest increase in follicular B cells and a large increase in marginal zone B cells
• CD4:CD8 T cell ratio is increased to 8:1 compared to 2:1 in controls
|
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• impaired reorganization of the cytoskeleton following cross-linking of surface IgM
• only weakly activated by anti-IgM but are activated by PMA/ionomycin
|
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• actin polymerization defects and activation defects following treatment with anti-CD3
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immune system
|
• disproportionate reduction in double positive thymocytes and accumulation of double negative and singly positive T cells
|
|
• weight is reduced even when smaller body weight is factored in
|
|
• contain more extensively segmented nuclei
|
|
• developmental block at the earliest stage of B cell development indicated by an increase in the proportion of pre-pro-B cells
|
|
• virtually absent from the peritoneal cavity
|
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• virtually absent from the peritoneal cavity
|
|
• greatest reductions are seen in the proportion of B220+ CD25+ pre-B cells
|
|
• red pulp surrounding lymphoid follicles is disorganized
|
small spleen
(
J:137415
)
|
• significantly smaller even when reduction in body size is factored in
|
|
• total cellularity is reduced to 12 +/- 7.1% of controls at 16 - 25 days of age
|
|
• lymphoid follicles are not clearly defined
|
|
• the proportion of B cells is reduced and the proportion of T cells is increased
• a disproportionate reduction in immature B cells is seen especially for T1 type cells accompanied by a modest increase in follicular B cells and a large increase in marginal zone B cells
• CD4:CD8 T cell ratio is increased to 8:1 compared to 2:1 in controls
|
|
• impaired reorganization of the cytoskeleton following cross-linking of surface IgM
• only weakly activated by anti-IgM but are activated by PMA/ionomycin
|
|
• actin polymerization defects and activation defects following treatment with anti-CD3
|
|
• sinuses are filled with mucus and about 40% of mice show signs of massive inflammation with cellular infiltrates and damage to the surrounding epithelium
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respiratory system
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• sinuses are filled with mucus and about 40% of mice show signs of massive inflammation with cellular infiltrates and damage to the surrounding epithelium
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endocrine/exocrine glands
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• disproportionate reduction in double positive thymocytes and accumulation of double negative and singly positive T cells
|
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• weight is reduced even when smaller body weight is factored in
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