All Phenotypes Igh<tm1Rbr> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Igh^tm1Rbr/Igh⁺	C57BL/6-Igh^tm1Rbr	MGI:3800461
cx2	Igh^tm1Rbr/Igh⁺ Tg(IgkHyHEL10)1Rbr/0	involves: C57BL/6	MGI:3800464
cx3	Igh^tm1Rbr/Igh⁺ Tg(IgkHyHEL10)1Rbr/0 Tg(ML5sHEL)5Ccg/0	involves: C57BL/6 * C57BL/6JSfd	MGI:3800476
cx4	Gpr183^tm1.2Rbr/Gpr183^tm1.2Rbr Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr/0	involves: C57BL/6 * FVB/N * SJL	MGI:4355855

Allelic Composition	Igh^tm1Rbr/Igh⁺
Genetic Background	C57BL/6-Igh^tm1Rbr

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm1Rbr mutation (4 available); any Igh mutation (44 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell morphology ( J:132538 )

• about 0.4% of B cells in the spleen produce immunoglobulins with high affinity for hen egg lysozyme antigen (HEL)

immune system

abnormal B cell morphology ( J:132538 )

• about 0.4% of B cells in the spleen produce immunoglobulins with high affinity for hen egg lysozyme antigen (HEL)

Allelic Composition	Igh^tm1Rbr/Igh⁺ Tg(IgkHyHEL10)1Rbr/0
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm1Rbr mutation (4 available); any Igh mutation (44 available)
Tg(IgkHyHEL10)1Rbr mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell morphology ( J:132538 )

• 40-60% of B cells in the spleen produce immunoglobulins that bind hen egg lysozyme antigen (HEL) with high affinity

increased immature B cell number ( J:132538 )

• the splenic B cells that bind HEL antigen are immature as indicated by the IgM^hi IgD^hi surface markers

abnormal B-2 B cell morphology ( J:132538 )

• all of the B cells found in the peritoneal cavity that bind HEL antigen are of the B-2 lineage as determined by B220^hi CD5^lowexpression

increased immunoglobulin level ( J:132538 )

increased IgA level ( J:132538 )

• there are low levels of anti-HEL IgA in the sera of unimmunized mice

increased IgG1 level ( J:132538 )

• there are low levels of anti-HEL IgG1 in the sera of unimmunized mice

increased IgG2a level ( J:132538 )

• there are low levels of anti-HEL IgG2a in the sera of unimmunized mice

increased IgG2b level ( J:132538 )

• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice

increased IgG3 level ( J:132538 )

• there are low levels of anti-HEL IgG3 in the sera of unimmunized mice

increased IgM level ( J:132538 )

• there are high levels of anti-HEL IgM in the sera of unimmunized mice

immune system

abnormal B cell morphology ( J:132538 )

• 40-60% of B cells in the spleen produce immunoglobulins that bind hen egg lysozyme antigen (HEL) with high affinity

increased immature B cell number ( J:132538 )

• the splenic B cells that bind HEL antigen are immature as indicated by the IgM^hi IgD^hi surface markers

abnormal B-2 B cell morphology ( J:132538 )

• all of the B cells found in the peritoneal cavity that bind HEL antigen are of the B-2 lineage as determined by B220^hi CD5^lowexpression

increased immunoglobulin level ( J:132538 )

increased IgA level ( J:132538 )

• there are low levels of anti-HEL IgA in the sera of unimmunized mice

increased IgG1 level ( J:132538 )

• there are low levels of anti-HEL IgG1 in the sera of unimmunized mice

increased IgG2a level ( J:132538 )

• there are low levels of anti-HEL IgG2a in the sera of unimmunized mice

increased IgG2b level ( J:132538 )

• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice

increased IgG3 level ( J:132538 )

• there are low levels of anti-HEL IgG3 in the sera of unimmunized mice

increased IgM level ( J:132538 )

• there are high levels of anti-HEL IgM in the sera of unimmunized mice

Allelic Composition	Igh^tm1Rbr/Igh⁺ Tg(IgkHyHEL10)1Rbr/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: C57BL/6 * C57BL/6JSfd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh^tm1Rbr mutation (4 available); any Igh mutation (44 available)
Tg(IgkHyHEL10)1Rbr mutation (4 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell morphology ( J:132538 )

• there is a 2-fold reduction in the number of HEL-specific B cells found in the spleen

increased immature B cell number ( J:132538 )

• HEL-specific B cells in the spleen have mostly have an immature phenotype

decreased follicular B cell number ( J:132538 )

• few HEL-specific B cells in the spleen are found in the follicular region or have surface markers indicative of follicular B cells

decreased marginal zone B cell number ( J:132538 )

• very few HEL-specific B cells in the spleen are found in the marginal zone or have surface markers indicative of the marginal zone phenotype

immune system

abnormal B cell morphology ( J:132538 )

• there is a 2-fold reduction in the number of HEL-specific B cells found in the spleen

increased immature B cell number ( J:132538 )

• HEL-specific B cells in the spleen have mostly have an immature phenotype

decreased follicular B cell number ( J:132538 )

• few HEL-specific B cells in the spleen are found in the follicular region or have surface markers indicative of follicular B cells

decreased marginal zone B cell number ( J:132538 )

• very few HEL-specific B cells in the spleen are found in the marginal zone or have surface markers indicative of the marginal zone phenotype

abnormal B cell anergy ( J:132538 )

• B cells are anergic when stimulated with HEL antigen as determined by reduced antibody production, no upregulation of activation markers CD69 and CD86, and failure to increase size

• these anergic B cells have a short half-life of less than three days

• however, these anergic B cells can proliferate in response to BCR-independent activation

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased B cell number ( J:151883 )

• 5 days following immunization with sheep red blood cell, B cells are observed at reduced frequency in the spleen compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased plasma cell number ( J:151883 )

• after activation with HEL-conjugated sheep red blood cell, fewer B cells acquired plasmablasts phenotype compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell physiology ( J:151883 )

• B cells fail to display a robust extrafollicular plasmablast response due to reduced migration of responding B cells to the bridging channel and red pulp arrears unlike in Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• however, expression of chemokine receptors is normal

impaired B cell migration ( J:151883 )

• 1 day after activation with HEL-conjugated sheep red blood cell, B cells do not distribute uniformly along the B-T boundary in the spleen and often migrate deep into T cell areas unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• 3 days after activation with HEL-conjugated sheep red blood cell, B cells fail to move to the poles of the B cell follicles proximal to bridging and interfollicular regions and instead remain localized in central follicular areas proximal to the T cell zone unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell proliferation ( J:151883 )

• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgG1 level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgG1 levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgM level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgM levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

hematopoietic system

decreased B cell number ( J:151883 )

• 5 days following immunization with sheep red blood cell, B cells are observed at reduced frequency in the spleen compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased plasma cell number ( J:151883 )

• after activation with HEL-conjugated sheep red blood cell, fewer B cells acquired plasmablasts phenotype compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell physiology ( J:151883 )

• however, expression of chemokine receptors is normal

impaired B cell migration ( J:151883 )

abnormal B cell proliferation ( J:151883 )

• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgG1 level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgG1 levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgM level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgM levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

cellular

impaired B cell migration ( J:151883 )

abnormal B cell proliferation ( J:151883 )

• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24