Phenotypes associated with this allele

• Allele Symbol: Tg(IgkHyHEL10)1Rbr
• Allele Name: transgene insertion 1, Robert Brink
• Allele ID: MGI:3800384
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Igh^tm1Rbr/Igh^+ Tg(IgkHyHEL10)1Rbr/0	involves: C57BL/6	MGI:3800464
cx2	Igh^tm1Rbr/Igh^+ Tg(IgkHyHEL10)1Rbr/0 Tg(ML5sHEL)5Ccg/0	involves: C57BL/6 * C57BL/6JSfd	MGI:3800476
cx3	Gpr183^tm1.2Rbr/Gpr183^tm1.2Rbr Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr/0	involves: C57BL/6 * FVB/N * SJL	MGI:4355855
tg4	Tg(IgkHyHEL10)1Rbr/0	involves: C57BL/6	MGI:3800463

Genotype
MGI:3800464

cx1

• Allelic Composition: Igh^tm1Rbr/Igh⁺; Tg(IgkHyHEL10)1Rbr/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal B cell morphology ( J:132538 )

• 40-60% of B cells in the spleen produce immunoglobulins that bind hen egg lysozyme antigen (HEL) with high affinity

increased immature B cell number ( J:132538 )

• the splenic B cells that bind HEL antigen are immature as indicated by the IgM^hi IgD^hi surface markers

abnormal B-2 B cell morphology ( J:132538 )

• all of the B cells found in the peritoneal cavity that bind HEL antigen are of the B-2 lineage as determined by B220^hi CD5^lowexpression

increased immunoglobulin level ( J:132538 )

increased IgA level ( J:132538 )

• there are low levels of anti-HEL IgA in the sera of unimmunized mice

increased IgG1 level ( J:132538 )

• there are low levels of anti-HEL IgG1 in the sera of unimmunized mice

increased IgG2a level ( J:132538 )

• there are low levels of anti-HEL IgG2a in the sera of unimmunized mice

increased IgG2b level ( J:132538 )

• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice

increased IgG3 level ( J:132538 )

• there are low levels of anti-HEL IgG3 in the sera of unimmunized mice

increased IgM level ( J:132538 )

• there are high levels of anti-HEL IgM in the sera of unimmunized mice

immune system

abnormal B cell morphology ( J:132538 )

• 40-60% of B cells in the spleen produce immunoglobulins that bind hen egg lysozyme antigen (HEL) with high affinity

increased immature B cell number ( J:132538 )

• the splenic B cells that bind HEL antigen are immature as indicated by the IgM^hi IgD^hi surface markers

abnormal B-2 B cell morphology ( J:132538 )

• all of the B cells found in the peritoneal cavity that bind HEL antigen are of the B-2 lineage as determined by B220^hi CD5^lowexpression

increased immunoglobulin level ( J:132538 )

increased IgA level ( J:132538 )

• there are low levels of anti-HEL IgA in the sera of unimmunized mice

increased IgG1 level ( J:132538 )

• there are low levels of anti-HEL IgG1 in the sera of unimmunized mice

increased IgG2a level ( J:132538 )

• there are low levels of anti-HEL IgG2a in the sera of unimmunized mice

increased IgG2b level ( J:132538 )

• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice

increased IgG3 level ( J:132538 )

• there are low levels of anti-HEL IgG3 in the sera of unimmunized mice

increased IgM level ( J:132538 )

• there are high levels of anti-HEL IgM in the sera of unimmunized mice

Genotype
MGI:3800476

cx2

• Allelic Composition: Igh^tm1Rbr/Igh⁺; Tg(IgkHyHEL10)1Rbr/0; Tg(ML5sHEL)5Ccg/0
• Genetic Background: involves: C57BL/6 * C57BL/6JSfd

hematopoietic system

abnormal B cell morphology ( J:132538 )

• there is a 2-fold reduction in the number of HEL-specific B cells found in the spleen

increased immature B cell number ( J:132538 )

• HEL-specific B cells in the spleen have mostly have an immature phenotype

decreased follicular B cell number ( J:132538 )

• few HEL-specific B cells in the spleen are found in the follicular region or have surface markers indicative of follicular B cells

decreased marginal zone B cell number ( J:132538 )

• very few HEL-specific B cells in the spleen are found in the marginal zone or have surface markers indicative of the marginal zone phenotype

immune system

abnormal B cell morphology ( J:132538 )

• there is a 2-fold reduction in the number of HEL-specific B cells found in the spleen

increased immature B cell number ( J:132538 )

• HEL-specific B cells in the spleen have mostly have an immature phenotype

decreased follicular B cell number ( J:132538 )

• few HEL-specific B cells in the spleen are found in the follicular region or have surface markers indicative of follicular B cells

decreased marginal zone B cell number ( J:132538 )

• very few HEL-specific B cells in the spleen are found in the marginal zone or have surface markers indicative of the marginal zone phenotype

abnormal B cell anergy ( J:132538 )

• B cells are anergic when stimulated with HEL antigen as determined by reduced antibody production, no upregulation of activation markers CD69 and CD86, and failure to increase size

• these anergic B cells have a short half-life of less than three days

• however, these anergic B cells can proliferate in response to BCR-independent activation

Genotype
MGI:4355855

cx3

• Allelic Composition: Gpr183^tm1.2Rbr/Gpr183^tm1.2Rbr; Igh^tm1Rbr/Igh^tm1Rbr; Tg(IgkHyHEL10)1Rbr/0
• Genetic Background: involves: C57BL/6 * FVB/N * SJL

immune system

decreased B cell number ( J:151883 )

• 5 days following immunization with sheep red blood cell, B cells are observed at reduced frequency in the spleen compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased plasma cell number ( J:151883 )

• after activation with HEL-conjugated sheep red blood cell, fewer B cells acquired plasmablasts phenotype compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell physiology ( J:151883 )

• B cells fail to display a robust extrafollicular plasmablast response due to reduced migration of responding B cells to the bridging channel and red pulp arrears unlike in Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• however, expression of chemokine receptors is normal

impaired B cell migration ( J:151883 )

• 1 day after activation with HEL-conjugated sheep red blood cell, B cells do not distribute uniformly along the B-T boundary in the spleen and often migrate deep into T cell areas unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• 3 days after activation with HEL-conjugated sheep red blood cell, B cells fail to move to the poles of the B cell follicles proximal to bridging and interfollicular regions and instead remain localized in central follicular areas proximal to the T cell zone unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell proliferation ( J:151883 )

• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgG1 level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgG1 levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgM level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgM levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

hematopoietic system

decreased B cell number ( J:151883 )

• 5 days following immunization with sheep red blood cell, B cells are observed at reduced frequency in the spleen compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased plasma cell number ( J:151883 )

• after activation with HEL-conjugated sheep red blood cell, fewer B cells acquired plasmablasts phenotype compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell physiology ( J:151883 )

• B cells fail to display a robust extrafollicular plasmablast response due to reduced migration of responding B cells to the bridging channel and red pulp arrears unlike in Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• however, expression of chemokine receptors is normal

impaired B cell migration ( J:151883 )

• 1 day after activation with HEL-conjugated sheep red blood cell, B cells do not distribute uniformly along the B-T boundary in the spleen and often migrate deep into T cell areas unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• 3 days after activation with HEL-conjugated sheep red blood cell, B cells fail to move to the poles of the B cell follicles proximal to bridging and interfollicular regions and instead remain localized in central follicular areas proximal to the T cell zone unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell proliferation ( J:151883 )

• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgG1 level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgG1 levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

decreased IgM level ( J:151883 )

• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgM levels are reduced to 10% to 20% of that in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

cellular

impaired B cell migration ( J:151883 )

• 1 day after activation with HEL-conjugated sheep red blood cell, B cells do not distribute uniformly along the B-T boundary in the spleen and often migrate deep into T cell areas unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

• 3 days after activation with HEL-conjugated sheep red blood cell, B cells fail to move to the poles of the B cell follicles proximal to bridging and interfollicular regions and instead remain localized in central follicular areas proximal to the T cell zone unlike in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

abnormal B cell proliferation ( J:151883 )

• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Igh^tm1Rbr/Igh^tm1Rbr Tg(IgkHyHEL10)1Rbr mice

Genotype
MGI:3800463

tg4

• Allelic Composition: Tg(IgkHyHEL10)1Rbr/0
• Genetic Background: involves: C57BL/6

normal phenotype

no abnormal phenotype detected ( J:132538 )