Phenotypes associated with this allele

• Allele Symbol: Tg(Prnp-Abca1)EHol
• Allele Name: transgene insertion E, David M Holtzman
• Allele ID: MGI:3800823
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Apoe^tm1Unc/Apoe^tm1Unc Tg(Prnp-Abca1)EHol/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3801012
cx2	Tg(APPV717F)109Ili/0 Tg(Prnp-Abca1)EHol/0	involves: C57BL/6 * CBA	MGI:3801011
tg3	Tg(Prnp-Abca1)EHol/0	involves: C57BL/6 * CBA	MGI:3801010

Genotype
MGI:3801012

cx1

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Tg(Prnp-Abca1)EHol/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

amyloid beta deposits ( J:131400 )

• frequent deposits are observed in hilus of dentate gyrus

• almost no thioflavin-S positive fibrillar Abeta plaques are observed in cortex and hippocampus

• mice show 2- to 3-fold less Abeta in the brain relative to Tg(APPV717F)109Ili, Apoe-wild-type controls

homeostasis/metabolism

amyloid beta deposits ( J:131400 )

• frequent deposits are observed in hilus of dentate gyrus

• almost no thioflavin-S positive fibrillar Abeta plaques are observed in cortex and hippocampus

• mice show 2- to 3-fold less Abeta in the brain relative to Tg(APPV717F)109Ili, Apoe-wild-type controls

Genotype
MGI:3801011

cx2

• Allelic Composition: Tg(APPV717F)109Ili/0; Tg(Prnp-Abca1)EHol/0
• Genetic Background: involves: C57BL/6 * CBA

nervous system

amyloid beta deposits ( J:131400 )

• at 12 months, rare amyloid beta (Abeta) deposits are observed in the cortex, with occasional deposits in the molecular layer of hippocampus and frequent Abeta deposits in the hilus of the dentate gyrus; mice show 3-fold less Abeta in the brain relative to Tg(APPV717F)109Ili, Abca1-wild-type controls

• fibrillar Abeta plaques are frequently observed in cortex and hippocampus

• Abeta deposits are almost all diffuse, with little true amyloid

• very few clusters of reactive microglia are seen in brain

homeostasis/metabolism

amyloid beta deposits ( J:131400 )

• at 12 months, rare amyloid beta (Abeta) deposits are observed in the cortex, with occasional deposits in the molecular layer of hippocampus and frequent Abeta deposits in the hilus of the dentate gyrus; mice show 3-fold less Abeta in the brain relative to Tg(APPV717F)109Ili, Abca1-wild-type controls

• fibrillar Abeta plaques are frequently observed in cortex and hippocampus

• Abeta deposits are almost all diffuse, with little true amyloid

• very few clusters of reactive microglia are seen in brain

Genotype
MGI:3801010

tg3

• Allelic Composition: Tg(Prnp-Abca1)EHol/0
• Genetic Background: involves: C57BL/6 * CBA

reproductive system

multinucleated giant male germ cells ( J:131400 )

♂

abnormal testis morphology ( J:131400 )

♂ • 3-fold increased numbers of apoptotic cells in testes relative to controls

seminiferous tubule degeneration ( J:131400 )

♂ • tubules display atrophy with marked degenerative changes such as large increase in multinucleated giant cells and cells with condensed nuclei

abnormal testis development ( J:131400 )

♂ • testes exhibit maturation arrest due to absence of mature products of spermatogenesis

decreased testis weight ( J:131400 )

♂ • 50% reduction in testes mass relative to wild-type controls

testicular atrophy ( J:131400 )

♂ • severe atrophy

abnormal spermatogenesis ( J:131400 )

♂ • elongated spermatids and spermatozoa are absent in testes of mutant males

male infertility ( J:131400 )

♂

nervous system

amyloid beta deposits ( J:131400 )

• in 12-month old mice have frequent amyloid beta (Abeta) deposits in cortex and hippocampus including molecular layer of hippocampus

• almost no deposits are observed in hilus of dentate gyrus of hippocampus

• thioflavin-S positive fibrillar Abeta plaques are frequently observed in cortex and hippocampus at 12 months

• thioflavin-S positive plaques are associated with reactive microglia

abnormal glial cell physiology ( J:131400 )

• Apoe-containing lipoprotein particles secreted by astrocytes contain more lipid than those secreted by non-transgenic controls; lipoprotein fractions contain a greater ratio of cholesterol to Apoe

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:131400 )

N • plasma levels of cholesterol including HDL-associated cholesterol and phospolipids, and Apoe are not significantly different from controls at 3 months

amyloid beta deposits ( J:131400 )

• in 12-month old mice have frequent amyloid beta (Abeta) deposits in cortex and hippocampus including molecular layer of hippocampus

• almost no deposits are observed in hilus of dentate gyrus of hippocampus

• thioflavin-S positive fibrillar Abeta plaques are frequently observed in cortex and hippocampus at 12 months

• thioflavin-S positive plaques are associated with reactive microglia

abnormal protein level ( J:131400 )

• mice show 40% reduction in Apoe levels in hippocampus at 3 months; in CSF, levels of Apoe are decreased >40%

• brain lysates contain significantly lower levels of Clusterin than control samples

endocrine/exocrine glands

abnormal testis morphology ( J:131400 )

♂ • 3-fold increased numbers of apoptotic cells in testes relative to controls

seminiferous tubule degeneration ( J:131400 )

♂ • tubules display atrophy with marked degenerative changes such as large increase in multinucleated giant cells and cells with condensed nuclei

abnormal testis development ( J:131400 )

♂ • testes exhibit maturation arrest due to absence of mature products of spermatogenesis

decreased testis weight ( J:131400 )

♂ • 50% reduction in testes mass relative to wild-type controls

testicular atrophy ( J:131400 )

♂ • severe atrophy

cellular

multinucleated giant male germ cells ( J:131400 )

♂