Phenotypes associated with this allele

• Allele Symbol: Tg(Wap-cre)51Nki
• Allele Name: transgene insertion 51, The Netherlands Cancer Institute
• Allele ID: MGI:3801314
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cdh1^tm1Jjon/Cdh1^tm1Jjon Tg(Wap-cre)51Nki/0	involves: 129P2/OlaHsd * FVB/N	MGI:6296605
cn2	Cdh1^tm1Jjon/Cdh1^tm1Jjon Trp53^tm1Brn/Trp53^tm1Brn Tg(Wap-cre)51Nki/0	involves: 129P2/OlaHsd * FVB/N	MGI:6296606
cn3	Trp53^tm1Brn/Trp53^tm1Brn Tg(Wap-cre)51Nki/0	involves: 129P2/OlaHsd * FVB/N	MGI:6296607
cn4	Cdh1^tm1Jjon/Cdh1^+ Trp53^tm1Brn/Trp53^tm1Brn Tg(Wap-cre)51Nki/0	involves: 129P2/OlaHsd * FVB/N	MGI:6296608
cn5	Cdh1^tm1Jjon/Cdh1^tm1Jjon Trp53^tm1Brn/Trp53^+ Tg(Wap-cre)51Nki/0	involves: 129P2/OlaHsd * FVB/N	MGI:6296609

Genotype
MGI:6296605

cn1

• Allelic Composition: Cdh1^tm1Jjon/Cdh1^tm1Jjon; Tg(Wap-cre)51Nki/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

hypolactation ( J:171765 )

♀ • the produced quantity of milk is lower than in wild-type mice, even though females lactate and are able to nurse their litters

♀ • however, no morphological abnormalities in the mammary gland are seen in virgin, pregnant or parous females

integument

hypolactation ( J:171765 )

♀ • the produced quantity of milk is lower than in wild-type mice, even though females lactate and are able to nurse their litters

♀ • however, no morphological abnormalities in the mammary gland are seen in virgin, pregnant or parous females

neoplasm

neoplasm ( J:171765 )

♀N • none of the mice develop mammary carcinomas, even after induction of multiple pregnancies

Genotype
MGI:6296606

cn2

• Allelic Composition: Cdh1^tm1Jjon/Cdh1^tm1Jjon; Trp53^tm1Brn/Trp53^tm1Brn; Tg(Wap-cre)51Nki/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

endocrine/exocrine glands

abnormal mammary gland growth during pregnancy ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show incomplete lobulo-alveolar development

♀ • as pregnancy progresses, the mammary gland fills with nonfunctional tissue resulting in complete disruption of the ductal structure at parturition

abnormal mammary gland duct morphology ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show severe ectasia (dilated ducts)

♀ • however, mammary glands from virgin mice show no gross morphological abnormalities

abnormal mammary gland lobule morphology ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show incomplete lobulo-alveolar development, with effects becoming more pronounced as pregnancy progresses

increased mammary gland tumor incidence ( J:171765 )

♀ • mice develop mammary tumors with a reduced tumor-free survival age of 194 days, with most tumors arising between 150 and 250 days of age

♀ • virgin females develop mammary tumors with identical incidence and latency as uniparous females

♀ • tumor spectrum, invasiveness and metastatic dissemination are similar in virgin and parous females

♀ • mice exhibit invasive mammary tumors which show phenotypic similarity to invasive lobular carcinoma and develop in high incidence multifocally in several mammary glands

♀ • most invasive lobular carcinomas are estrogen receptor negative

♀ • females also exhibit solid carcinoma/carcinosarcoma that predominantly show a mixed epithelial and mesenchymal or spindle-shaped cell morphology and show both expansive and invasive growth patterns

lactation failure ( J:171765 )

♀ • mice produce healthy newborns, however all pups fostered by them die before weaning due to starvation

integument

abnormal mammary gland growth during pregnancy ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show incomplete lobulo-alveolar development

♀ • as pregnancy progresses, the mammary gland fills with nonfunctional tissue resulting in complete disruption of the ductal structure at parturition

abnormal mammary gland duct morphology ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show severe ectasia (dilated ducts)

♀ • however, mammary glands from virgin mice show no gross morphological abnormalities

abnormal mammary gland lobule morphology ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show incomplete lobulo-alveolar development, with effects becoming more pronounced as pregnancy progresses

increased mammary gland tumor incidence ( J:171765 )

♀ • mice develop mammary tumors with a reduced tumor-free survival age of 194 days, with most tumors arising between 150 and 250 days of age

♀ • virgin females develop mammary tumors with identical incidence and latency as uniparous females

♀ • tumor spectrum, invasiveness and metastatic dissemination are similar in virgin and parous females

♀ • mice exhibit invasive mammary tumors which show phenotypic similarity to invasive lobular carcinoma and develop in high incidence multifocally in several mammary glands

♀ • most invasive lobular carcinomas are estrogen receptor negative

♀ • females also exhibit solid carcinoma/carcinosarcoma that predominantly show a mixed epithelial and mesenchymal or spindle-shaped cell morphology and show both expansive and invasive growth patterns

lactation failure ( J:171765 )

♀ • mice produce healthy newborns, however all pups fostered by them die before weaning due to starvation

neoplasm

increased mammary gland tumor incidence ( J:171765 )

♀ • mice develop mammary tumors with a reduced tumor-free survival age of 194 days, with most tumors arising between 150 and 250 days of age

♀ • virgin females develop mammary tumors with identical incidence and latency as uniparous females

♀ • tumor spectrum, invasiveness and metastatic dissemination are similar in virgin and parous females

♀ • mice exhibit invasive mammary tumors which show phenotypic similarity to invasive lobular carcinoma and develop in high incidence multifocally in several mammary glands

♀ • most invasive lobular carcinomas are estrogen receptor negative

♀ • females also exhibit solid carcinoma/carcinosarcoma that predominantly show a mixed epithelial and mesenchymal or spindle-shaped cell morphology and show both expansive and invasive growth patterns

increased metastatic potential ( J:171765 )

♀ • 74% of females that present mammary tumors of about 1 cm show extensive local invasion and metastases to draining and distant lymph nodes

♀ • invasive lobular carcinoma cells are seen in skin, lungs, liver, gastrointestinal tract, pancreas, and spleen or are diffusely disseminated throughout the peritoneal cavity

♀ • several mice develop bone metastases

reproductive system

abnormal mammary gland growth during pregnancy ( J:171765 )

♀ • mammary glands from females at day 14 of pregnancy show incomplete lobulo-alveolar development

♀ • as pregnancy progresses, the mammary gland fills with nonfunctional tissue resulting in complete disruption of the ductal structure at parturition

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
invasive lobular carcinoma		DOID:3457		J:171765

Genotype
MGI:6296607

cn3

• Allelic Composition: Trp53^tm1Brn/Trp53^tm1Brn; Tg(Wap-cre)51Nki/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

endocrine/exocrine glands

increased mammary gland tumor incidence ( J:171765 )

♀ • all females develop mammary tumors with a median latency of 290 days

♀ • mammary tumors are either intermediate-grade adenocarcinoma or solid carcinoma/carcinosarcoma

♀ • tumors show benign noninvasive features and consist of large epithelial cells forming solid nests or irregular glands

♀ • tumors only sporadically metastasize to the lung

increased mammary adenocarcinoma incidence ( J:171765 )

♀ • some mammary tumors are intermediate-grade adenocarcinoma

integument

increased mammary gland tumor incidence ( J:171765 )

♀ • all females develop mammary tumors with a median latency of 290 days

♀ • mammary tumors are either intermediate-grade adenocarcinoma or solid carcinoma/carcinosarcoma

♀ • tumors show benign noninvasive features and consist of large epithelial cells forming solid nests or irregular glands

♀ • tumors only sporadically metastasize to the lung

increased mammary adenocarcinoma incidence ( J:171765 )

♀ • some mammary tumors are intermediate-grade adenocarcinoma

neoplasm

increased mammary gland tumor incidence ( J:171765 )

♀ • all females develop mammary tumors with a median latency of 290 days

♀ • mammary tumors are either intermediate-grade adenocarcinoma or solid carcinoma/carcinosarcoma

♀ • tumors show benign noninvasive features and consist of large epithelial cells forming solid nests or irregular glands

♀ • tumors only sporadically metastasize to the lung

increased mammary adenocarcinoma incidence ( J:171765 )

♀ • some mammary tumors are intermediate-grade adenocarcinoma

Genotype
MGI:6296608

cn4

• Allelic Composition: Cdh1^tm1Jjon/Cdh1⁺; Trp53^tm1Brn/Trp53^tm1Brn; Tg(Wap-cre)51Nki/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

endocrine/exocrine glands

increased mammary gland tumor incidence ( J:171765 )

♀ • females develop mammary tumors with a median latency of around 290 days

♀ • mammary tumors are either intermediate-grade adenocarcinoma or solid carcinoma/carcinosarcoma

♀ • virgin females develop mammary tumors with identical incidence and latency as uniparous females

♀ • tumor spectrum, invasiveness and metastatic dissemination are similar in virgin and parous females

increased mammary adenocarcinoma incidence ( J:171765 )

♀ • some mice develop intermediate-grade adenocarcinoma

integument

increased mammary gland tumor incidence ( J:171765 )

♀ • females develop mammary tumors with a median latency of around 290 days

♀ • mammary tumors are either intermediate-grade adenocarcinoma or solid carcinoma/carcinosarcoma

♀ • virgin females develop mammary tumors with identical incidence and latency as uniparous females

♀ • tumor spectrum, invasiveness and metastatic dissemination are similar in virgin and parous females

increased mammary adenocarcinoma incidence ( J:171765 )

♀ • some mice develop intermediate-grade adenocarcinoma

neoplasm

increased mammary gland tumor incidence ( J:171765 )

♀ • females develop mammary tumors with a median latency of around 290 days

♀ • mammary tumors are either intermediate-grade adenocarcinoma or solid carcinoma/carcinosarcoma

♀ • virgin females develop mammary tumors with identical incidence and latency as uniparous females

♀ • tumor spectrum, invasiveness and metastatic dissemination are similar in virgin and parous females

increased mammary adenocarcinoma incidence ( J:171765 )

♀ • some mice develop intermediate-grade adenocarcinoma

Genotype
MGI:6296609

cn5

• Allelic Composition: Cdh1^tm1Jjon/Cdh1^tm1Jjon; Trp53^tm1Brn/Trp53⁺; Tg(Wap-cre)51Nki/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

endocrine/exocrine glands

increased mammary gland tumor incidence ( J:171765 )

♀ • mice exhibit invasive mammary tumors which show phenotypic similarity to invasive lobular carcinoma

♀ • females also develop solid carcinoma/carcinosarcoma that predominantly exhibit a mixed epithelial and mesenchymal or spindle-shaped cell morphology and show both expansive and invasive growth patterns

hypolactation ( J:171765 )

♀ • pups show reduced survival rates due to inhibition or absence of lactation

integument

increased mammary gland tumor incidence ( J:171765 )

♀ • mice exhibit invasive mammary tumors which show phenotypic similarity to invasive lobular carcinoma

♀ • females also develop solid carcinoma/carcinosarcoma that predominantly exhibit a mixed epithelial and mesenchymal or spindle-shaped cell morphology and show both expansive and invasive growth patterns

hypolactation ( J:171765 )

♀ • pups show reduced survival rates due to inhibition or absence of lactation

neoplasm

increased mammary gland tumor incidence ( J:171765 )

♀ • mice exhibit invasive mammary tumors which show phenotypic similarity to invasive lobular carcinoma

♀ • females also develop solid carcinoma/carcinosarcoma that predominantly exhibit a mixed epithelial and mesenchymal or spindle-shaped cell morphology and show both expansive and invasive growth patterns

increased metastatic potential ( J:171765 )

♀ • 70% of females that present mammary tumors of about 1 cm show extensive local invasion and metastases to draining and distant lymph nodes