Analysis Tools
mortality/aging
|
• deaths occur by the second week and no mice survive beyond 30 days
|
growth/size/body
|
• normal size at birth
• growth retardation apparent by day 7
• organ weights of heart, liver, spleen, and kidney are normal through day 12
|
homeostasis/metabolism
|
• exhibit shivering
|
|
• colder than normal littermates
• become progressively and severely hypothermic from 10 days of age
|
cellular
|
• 40-80% reduction in mitochondrial DNA levels in all organs investigated
• skeletal muscle, heart, liver, and spleen with normal mitochondrial DNA levels at birth but progressive loss during the first two weeks of life
• mitochondrial DNA levels already low at birth in the brain and adipose tissue
• mutation rate for mitochondrial DNA is normal
|
|
• heart mitochondria show disrupted cristae at 14 days
|
cardiovascular system
|
• muscle fibers in the heart are disorganized
|
liver/biliary system
|
• increased numbers of intracellular small lipid vesicles at 14 days of age
|
adipose tissue
|
• near complete absence of hypodermal fat layer at 14 days
|
|
• altered structure of interscapular brown fat
• heterogeneity of cell size and accumulation of lipid vesicles at 14 days
|
muscle
|
• muscle fibers in the heart are disorganized
|
integument
|
• near complete absence of hypodermal fat layer at 14 days
|
