Phenotypes associated with this allele

• Allele Symbol: Igkc^tm1Jkbv
• Allele Name: targeted mutation 1, Aya Jakobovits
• Allele ID: MGI:3802625
• Summary: 16 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igkc^tm1Jkbv/Igkc^tm1Jkbv	Not Specified	MGI:3802929
cx2	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH1)2BJkbv/0 Tg(IGK@yK1)9.2CJkbv/0	involves: 129P2/OlaHsd	MGI:3802681
cx3	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv	involves: 129P2/OlaHsd	MGI:3802682
cx4	Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGK@yK1)9.2CJkbv/0	involves: 129P2/OlaHsd	MGI:3802930
cx5	Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv	involves: 129P2/OlaHsd	MGI:3802931
cx6	Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGK@yK2)J23.1Jkbv/0	involves: 129P2/OlaHsd	MGI:3802932
cx7	Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv	involves: 129P2/OlaHsd	MGI:3802933
cx8	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH1)2BJkbv/0 Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv	involves: 129P2/OlaHsd	MGI:3802934
cx9	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH1)2BJkbv/Tg(IGH@yH1)2BJkbv Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv	involves: 129P2/OlaHsd	MGI:3802935
cx10	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH1)2BJkbv/Tg(IGH@yH1)2BJkbv Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv	involves: 129P2/OlaHsd	MGI:3802936
cx11	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH2)J9.2Jkbv/0 Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv	involves: 129P2/OlaHsd	MGI:3802937
cx12	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH2BM)7Amgn/0 Tg(IGK@yK2)J23.1Jkbv/0	involves: 129P2/OlaHsd	MGI:3810268
cx13	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH2CM)2Amgn/0 Tg(IGK@yK2)J23.1Jkbv/0	involves: 129P2/OlaHsd	MGI:3810269
cx14	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yHG1/2)1Amgn/0 Tg(IGK@yK2)J23.1Jkbv/0	involves: 129P2/OlaHsd	MGI:3810270
cx15	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yHG4)1Amgn/0 Tg(IGK@yK2)J23.1Jkbv/0	involves: 129P2/OlaHsd	MGI:3810271
cx16	Igh-J^tm1Jkbv/Igh-J^tm1Jkbv Igkc^tm1Jkbv/Igkc^tm1Jkbv Tg(IGH@yH2)J9.2Jkbv/0 Tg(IGK@yK2)J23.1Jkbv/0	involves: 129P2/OlaHsd * C57BL/6J	MGI:3802734

Genotype
MGI:3802929

hm1

• Allelic Composition: Igkc^tm1Jkbv/Igkc^tm1Jkbv
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal B cell differentiation ( J:137738 )

• B cells exclusively express lambda light chain and not kappa light chain

decreased immature B cell number ( J:137738 )

• immature B cells in circulation are at 50-60% of wild-type levels

abnormal immunoglobulin light chain V-J recombination ( J:137738 )

• only lambda light chains are productively rearranged

decreased mature B cell number ( J:137738 )

• mature B cells are at 50-60% of wild-type levels in the spleen, blood, and lymph nodes

increased pre-B cell number ( J:137738 )

• there is a 2-fold accumulation of small pre- B cells in the bone marrow

abnormal immunoglobulin level ( J:137738 )

• the concentration of antibodies containing lamda light chain is 580 micrograms per ml compared to 70 micrograms per ml in wild-type mice

hematopoietic system

abnormal B cell differentiation ( J:137738 )

• B cells exclusively express lambda light chain and not kappa light chain

decreased immature B cell number ( J:137738 )

• immature B cells in circulation are at 50-60% of wild-type levels

abnormal immunoglobulin light chain V-J recombination ( J:137738 )

• only lambda light chains are productively rearranged

decreased mature B cell number ( J:137738 )

• mature B cells are at 50-60% of wild-type levels in the spleen, blood, and lymph nodes

increased pre-B cell number ( J:137738 )

• there is a 2-fold accumulation of small pre- B cells in the bone marrow

abnormal immunoglobulin level ( J:137738 )

• the concentration of antibodies containing lamda light chain is 580 micrograms per ml compared to 70 micrograms per ml in wild-type mice

Genotype
MGI:3802681

cx2

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH1)2BJkbv/0; Tg(IGK@yK1)9.2CJkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal B cell morphology ( J:23827 )

• 43% of B cells express human mu heavy chain and human kappa light chain

• 57% of B cells express human mu heavy chain and mouse lamda light chain

• no co-expression of human and mouse light chain is found

increased mature B cell number ( J:23827 )

• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-J^tm1Jkbv Igk-C^tm1Jkbv double homozygotes

• mature B cells are found at a level 10% of that in wild-type mice

abnormal immunoglobulin level ( J:23827 )

• human antibodies occur in the sera of mice at 10 micrograms per ml

• this concentration is several hundred fold higher than occurs in transgenic mice that do not have endogenous Ig heavy and light chains inactivated

hematopoietic system

abnormal B cell morphology ( J:23827 )

• 43% of B cells express human mu heavy chain and human kappa light chain

• 57% of B cells express human mu heavy chain and mouse lamda light chain

• no co-expression of human and mouse light chain is found

increased mature B cell number ( J:23827 )

• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-J^tm1Jkbv Igk-C^tm1Jkbv double homozygotes

• mature B cells are found at a level 10% of that in wild-type mice

abnormal immunoglobulin level ( J:23827 )

• human antibodies occur in the sera of mice at 10 micrograms per ml

• this concentration is several hundred fold higher than occurs in transgenic mice that do not have endogenous Ig heavy and light chains inactivated

Genotype
MGI:3802682

cx3

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type

arrested B cell differentiation ( J:23827 , J:137738 )

absent mature B cells ( J:70620 , J:137738 )

• mature B cells are absent from the spleen and blood (J:70620)

• mature B cells are not found in the periphery (J:137738)

absent B cells ( J:23827 )

• B cells are not found in this mouse strain

hematopoietic system

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type

arrested B cell differentiation ( J:23827 , J:137738 )

absent mature B cells ( J:70620 , J:137738 )

• mature B cells are absent from the spleen and blood (J:70620)

• mature B cells are not found in the periphery (J:137738)

absent B cells ( J:23827 )

• B cells are not found in this mouse strain

Genotype
MGI:3802930

cx4

• Allelic Composition: Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGK@yK1)9.2CJkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

decreased immature B cell number ( J:137738 )

• immature B cells in circulation are at 70% of wild-type levels

decreased mature B cell number ( J:137738 )

• mature B cells are at 50-60% of wild-type levels in the spleen

• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-C^tm1Jkbv homozygotes

• mature B cells expressing human kappa light chain are found in similar numbers as B cells expressing murine lambda light chain

increased pre-B cell number ( J:137738 )

• there is a 2-fold accumulation of small pre- B cells in the bone marrow

abnormal immunoglobulin level ( J:137738 )

• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-C^tm1Jkbv homozygotes

• the concentration of antibodies containing human kappa light chain is 250 micrograms per ml in sera

hematopoietic system

decreased immature B cell number ( J:137738 )

• immature B cells in circulation are at 70% of wild-type levels

decreased mature B cell number ( J:137738 )

• mature B cells are at 50-60% of wild-type levels in the spleen

• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-C^tm1Jkbv homozygotes

• mature B cells expressing human kappa light chain are found in similar numbers as B cells expressing murine lambda light chain

increased pre-B cell number ( J:137738 )

• there is a 2-fold accumulation of small pre- B cells in the bone marrow

abnormal immunoglobulin level ( J:137738 )

• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-C^tm1Jkbv homozygotes

• the concentration of antibodies containing human kappa light chain is 250 micrograms per ml in sera

Genotype
MGI:3802931

cx5

• Allelic Composition: Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal B cell differentiation ( J:137738 )

• B cells exclusively express lambda light chain and not kappa light chain

decreased immature B cell number ( J:137738 )

• immature B cells in circulation are at 70% of wild-type levels

decreased mature B cell number ( J:137738 )

• mature B cells are at 50-60% of wild-type levels in the spleen

• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-C^tm1Jkbv homozygotes

• mature B cells expressing human kappa light chain are twice as common as B cells expressing murine lambda light chain

increased pre-B cell number ( J:137738 )

• there is a 2-fold accumulation of small pre- B cells in the bone marrow

abnormal immunoglobulin level ( J:137738 )

• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-C^tm1Jkbv homozygotes

• the concentration of antibodies containing human kappa light chain is 720 micrograms per ml in sera

hematopoietic system

abnormal B cell differentiation ( J:137738 )

• B cells exclusively express lambda light chain and not kappa light chain

decreased immature B cell number ( J:137738 )

• immature B cells in circulation are at 70% of wild-type levels

decreased mature B cell number ( J:137738 )

• mature B cells are at 50-60% of wild-type levels in the spleen

• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-C^tm1Jkbv homozygotes

• mature B cells expressing human kappa light chain are twice as common as B cells expressing murine lambda light chain

increased pre-B cell number ( J:137738 )

• there is a 2-fold accumulation of small pre- B cells in the bone marrow

abnormal immunoglobulin level ( J:137738 )

• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-C^tm1Jkbv homozygotes

• the concentration of antibodies containing human kappa light chain is 720 micrograms per ml in sera

Genotype
MGI:3802932

cx6

• Allelic Composition: Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGK@yK2)J23.1Jkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

immune system phenotype ( J:137738 )

N • the presence of the transgene prevents the partial arrest of B cell development that occurs in Igk-C^tm1Jkbv homozygotes

N • normal numbers of B cells are found at all developmental stages including immature and mature stages

abnormal B cell morphology ( J:137738 )

• 75% of mature B cells express human kappa light chain

abnormal immunoglobulin level ( J:137738 )

• levels of antibody containing human kappa light chain are 1,400 micrograms per ml while levels of antibody containing murine lambda light chain are 100 micrograms per ml

hematopoietic system

abnormal B cell morphology ( J:137738 )

• 75% of mature B cells express human kappa light chain

abnormal immunoglobulin level ( J:137738 )

• levels of antibody containing human kappa light chain are 1,400 micrograms per ml while levels of antibody containing murine lambda light chain are 100 micrograms per ml

Genotype
MGI:3802933

cx7

• Allelic Composition: Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

immune system phenotype ( J:137738 )

N • the presence of the transgene prevents the partial arrest of B cell development that occurs in Igk-C^tm1Jkbv homozygotes

N • normal numbers of B cells are found at all developmental stages including immature and mature stages

abnormal B cell morphology ( J:137738 )

• over 90% of mature B cells express human kappa light chain

hematopoietic system

abnormal B cell morphology ( J:137738 )

• over 90% of mature B cells express human kappa light chain

Genotype
MGI:3802934

cx8

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH1)2BJkbv/0; Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal B cell differentiation ( J:137738 )

• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells

decreased immature B cell number ( J:137738 )

• B cells newly emerging from the bone marrow are at levels that are 10% of that in wild-type mice

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43⁺ CD45⁺ HSA^med BP-1⁺) step

decreased mature B cell number ( J:137738 )

• the levels of circulating mature B cells are 10% of the numbers found in wild-type

• these B cells express human mu heavy chain

decreased pre-B cell number ( J:137738 )

• pre-B cells numbers in the bone marrow are 30% of wild-type

hematopoietic system

abnormal B cell differentiation ( J:137738 )

• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells

decreased immature B cell number ( J:137738 )

• B cells newly emerging from the bone marrow are at levels that are 10% of that in wild-type mice

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43⁺ CD45⁺ HSA^med BP-1⁺) step

decreased mature B cell number ( J:137738 )

• the levels of circulating mature B cells are 10% of the numbers found in wild-type

• these B cells express human mu heavy chain

decreased pre-B cell number ( J:137738 )

• pre-B cells numbers in the bone marrow are 30% of wild-type

Genotype
MGI:3802935

cx9

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH1)2BJkbv/Tg(IGH@yH1)2BJkbv; Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal B cell differentiation ( J:137738 )

• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells

decreased immature B cell number ( J:137738 )

• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43⁺ CD45⁺ HSA^med BP-1⁺) step

decreased mature B cell number ( J:137738 )

• the levels of circulating mature B cells are 10% of the numbers found in wild-type

• these B cells express human mu heavy chain

decreased pre-B cell number ( J:137738 )

• pre-B cells numbers in the bone marrow are 70% of wild-type

abnormal immunoglobulin level ( J:137738 )

• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 150 and 120 micrograms per ml, respectively

hematopoietic system

abnormal B cell differentiation ( J:137738 )

• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells

decreased immature B cell number ( J:137738 )

• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43⁺ CD45⁺ HSA^med BP-1⁺) step

decreased mature B cell number ( J:137738 )

• the levels of circulating mature B cells are 10% of the numbers found in wild-type

• these B cells express human mu heavy chain

decreased pre-B cell number ( J:137738 )

• pre-B cells numbers in the bone marrow are 70% of wild-type

abnormal immunoglobulin level ( J:137738 )

• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 150 and 120 micrograms per ml, respectively

Genotype
MGI:3802936

cx10

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH1)2BJkbv/Tg(IGH@yH1)2BJkbv; Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal B cell differentiation ( J:137738 )

• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells

decreased immature B cell number ( J:137738 )

• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43⁺ CD45⁺ HSA^med BP-1⁺) step

decreased mature B cell number ( J:137738 )

• the levels of circulating mature B cells are 10% of the numbers found in wild-type

• these B cells express human mu heavy chain

decreased pre-B cell number ( J:137738 )

• pre-B cells numbers in the bone marrow are 70% of wild-type

hematopoietic system

abnormal B cell differentiation ( J:137738 )

• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells

decreased immature B cell number ( J:137738 )

• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice

increased late pro-B cell number ( J:137738 )

• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43⁺ CD45⁺ HSA^med BP-1⁺) step

decreased mature B cell number ( J:137738 )

• the levels of circulating mature B cells are 10% of the numbers found in wild-type

• these B cells express human mu heavy chain

decreased pre-B cell number ( J:137738 )

• pre-B cells numbers in the bone marrow are 70% of wild-type

Genotype
MGI:3802937

cx11

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH2)J9.2Jkbv/0; Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
• Genetic Background: involves: 129P2/OlaHsd

immune system

immune system phenotype ( J:137738 )

N • the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-J^tm1Jkbv homozygotes

decreased immature B cell number ( J:137738 )

• the levels of immature B cells found in circulation are 70% that found in wild-type mice

decreased mature B cell number ( J:137738 )

• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice

• these B cells express human mu heavy chain

abnormal immunoglobulin level ( J:137738 )

• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively

hematopoietic system

decreased immature B cell number ( J:137738 )

• the levels of immature B cells found in circulation are 70% that found in wild-type mice

decreased mature B cell number ( J:137738 )

• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice

• these B cells express human mu heavy chain

abnormal immunoglobulin level ( J:137738 )

• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively

Genotype
MGI:3810268

cx12

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH2BM)7Amgn/0; Tg(IGK@yK2)J23.1Jkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

decreased B cell number ( J:139792 )

• mice have 60-80% the number of B cells as wild-type mice

abnormal immunoglobulin level ( J:139792 )

• unimmunized mice produce significant levels of human IgM and IgG1

• IgM levels in sera ranged from 110-140 micrograms/ml

• IgG1 levels in sera ranged from 172-298 micrograms/ml

• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences

hematopoietic system

decreased B cell number ( J:139792 )

• mice have 60-80% the number of B cells as wild-type mice

abnormal immunoglobulin level ( J:139792 )

• unimmunized mice produce significant levels of human IgM and IgG1

• IgM levels in sera ranged from 110-140 micrograms/ml

• IgG1 levels in sera ranged from 172-298 micrograms/ml

• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences

Genotype
MGI:3810269

cx13

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH2CM)2Amgn/0; Tg(IGK@yK2)J23.1Jkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

decreased B cell number ( J:139792 )

• mice have 80-100% the number of B cells as wild-type mice

abnormal immunoglobulin level ( J:139792 )

• unimmunized mice produce significant levels of human IgM and IgG2

• IgM levels in sera ranged from 27.3-109.4 micrograms/ml

• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml

• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences

hematopoietic system

decreased B cell number ( J:139792 )

• mice have 80-100% the number of B cells as wild-type mice

abnormal immunoglobulin level ( J:139792 )

• unimmunized mice produce significant levels of human IgM and IgG2

• IgM levels in sera ranged from 27.3-109.4 micrograms/ml

• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml

• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences

Genotype
MGI:3810270

cx14

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yHG1/2)1Amgn/0; Tg(IGK@yK2)J23.1Jkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal immunoglobulin level ( J:139792 )

• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences

hematopoietic system

abnormal immunoglobulin level ( J:139792 )

• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences

Genotype
MGI:3810271

cx15

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yHG4)1Amgn/0; Tg(IGK@yK2)J23.1Jkbv/0
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal immunoglobulin level ( J:139792 )

• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences

hematopoietic system

abnormal immunoglobulin level ( J:139792 )

• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences

Genotype
MGI:3802734

cx16

• Allelic Composition: Igh-J^tm1Jkbv/Igh-J^tm1Jkbv; Igkc^tm1Jkbv/Igkc^tm1Jkbv; Tg(IGH@yH2)J9.2Jkbv/0; Tg(IGK@yK2)J23.1Jkbv/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

immune system

abnormal B cell morphology ( J:70620 )

• all B cells express human IgM and high levels of B220

• about 80% of B cells exclusively express human kappa light chain

• only 15% of B cells express endogenous mouse lambda mouse chain

abnormal immunoglobulin heavy chain V(D)J recombination ( J:70620 )

• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells

• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans

• J segment usage also resembles usage in human B cells

abnormal immunoglobulin light chain V-J recombination ( J:70620 )

• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells

• V and J gene segment usage resembles what is observed in human B cells

increased mature B cell number ( J:70620 )

• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-J^tm1Jkbv Igk-C^tm1Jkbv double homozygotes

• mature B cell numbers are 60% of that found in wild-type mice

abnormal immunoglobulin level ( J:70620 )

• antibodies containing human immunoglobulin chains are present in the sera

• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively

• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml

• high affinity human antibodies can be generated by immunizing mice with human antigen

hematopoietic system

abnormal B cell morphology ( J:70620 )

• all B cells express human IgM and high levels of B220

• about 80% of B cells exclusively express human kappa light chain

• only 15% of B cells express endogenous mouse lambda mouse chain

abnormal immunoglobulin heavy chain V(D)J recombination ( J:70620 )

• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells

• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans

• J segment usage also resembles usage in human B cells

abnormal immunoglobulin light chain V-J recombination ( J:70620 )

• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells

• V and J gene segment usage resembles what is observed in human B cells

increased mature B cell number ( J:70620 )

• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-J^tm1Jkbv Igk-C^tm1Jkbv double homozygotes

• mature B cell numbers are 60% of that found in wild-type mice

abnormal immunoglobulin level ( J:70620 )

• antibodies containing human immunoglobulin chains are present in the sera

• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively

• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml

• high affinity human antibodies can be generated by immunizing mice with human antigen