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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(IGK@yK1)9.2CJkbv
transgene insertion 9.2C, Aya Jakobovits
MGI:3802658
Summary 8 genotypes


Genotype
MGI:3802679
cx1
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Tg(IGH@yH1)2BJkbv/0
Tg(IGK@yK1)9.2CJkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Tg(IGH@yH1)2BJkbv mutation (0 available)
Tg(IGK@yK1)9.2CJkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal B cell morphology ( J:23827 )
• all B cells express human mu heavy chain immunoglobulin with most of the B cells also expressing human delta heavy chain
increased late pro-B cell number ( J:23827 )
• late pro-B cell numbers are increased more than 2-fold compared to wild-type mice suggesting a less efficient V to DJ joining process
increased mature B cell number ( J:23827 )
• the presence of the two transgenes rescues the block in B cell development that occurs in Igh-Jtm1Jkbv development
• mature B cells are found in the bone marrow, spleen and blood in numbers corresponding to 58%, 55%, and 30% of wild-type levels
abnormal immunoglobulin level ( J:23827 )
• antibodies containing human mu heavy chain are found in the serum at a concentration of 350 micrograms per ml, a level over 500 fold higher than found in transgenic mice without the Igh-J targeted mutation
• the serum concentration of antibodies with human mu heavy chain is only 2- to 3- fold lower than the concentration of IgM found in humans

immune system
abnormal B cell morphology ( J:23827 )
• all B cells express human mu heavy chain immunoglobulin with most of the B cells also expressing human delta heavy chain
increased late pro-B cell number ( J:23827 )
• late pro-B cell numbers are increased more than 2-fold compared to wild-type mice suggesting a less efficient V to DJ joining process
increased mature B cell number ( J:23827 )
• the presence of the two transgenes rescues the block in B cell development that occurs in Igh-Jtm1Jkbv development
• mature B cells are found in the bone marrow, spleen and blood in numbers corresponding to 58%, 55%, and 30% of wild-type levels
abnormal immunoglobulin level ( J:23827 )
• antibodies containing human mu heavy chain are found in the serum at a concentration of 350 micrograms per ml, a level over 500 fold higher than found in transgenic mice without the Igh-J targeted mutation
• the serum concentration of antibodies with human mu heavy chain is only 2- to 3- fold lower than the concentration of IgM found in humans




Genotype
MGI:3802680
cx2
Allelic
Composition		 Tg(IGH@yH1)2BJkbv/0
Tg(IGK@yK1)9.2CJkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell morphology ( J:23827 )
• 0.8-3.9% of B cells express human mu heavy chain and 1.4-4.8% express human kappa chain
• 0.06% to 0.27% of B cells express both the human heavy and light chains
abnormal immunoglobulin level ( J:23827 )
• human antibodies are found in the serum at 0.3 to 0.7 micrograms per ml
• antigen specific human antibodies are generated when mice are immunized with tetanus toxin fragment

hematopoietic system
abnormal B cell morphology ( J:23827 )
• 0.8-3.9% of B cells express human mu heavy chain and 1.4-4.8% express human kappa chain
• 0.06% to 0.27% of B cells express both the human heavy and light chains
abnormal immunoglobulin level ( J:23827 )
• human antibodies are found in the serum at 0.3 to 0.7 micrograms per ml
• antigen specific human antibodies are generated when mice are immunized with tetanus toxin fragment




Genotype
MGI:3802681
cx3
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH1)2BJkbv/0
Tg(IGK@yK1)9.2CJkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH1)2BJkbv mutation (0 available)
Tg(IGK@yK1)9.2CJkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell morphology ( J:23827 )
• 43% of B cells express human mu heavy chain and human kappa light chain
• 57% of B cells express human mu heavy chain and mouse lamda light chain
• no co-expression of human and mouse light chain is found
increased mature B cell number ( J:23827 )
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cells are found at a level 10% of that in wild-type mice
abnormal immunoglobulin level ( J:23827 )
• human antibodies occur in the sera of mice at 10 micrograms per ml
• this concentration is several hundred fold higher than occurs in transgenic mice that do not have endogenous Ig heavy and light chains inactivated

hematopoietic system
abnormal B cell morphology ( J:23827 )
• 43% of B cells express human mu heavy chain and human kappa light chain
• 57% of B cells express human mu heavy chain and mouse lamda light chain
• no co-expression of human and mouse light chain is found
increased mature B cell number ( J:23827 )
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cells are found at a level 10% of that in wild-type mice
abnormal immunoglobulin level ( J:23827 )
• human antibodies occur in the sera of mice at 10 micrograms per ml
• this concentration is several hundred fold higher than occurs in transgenic mice that do not have endogenous Ig heavy and light chains inactivated




Genotype
MGI:3802930
cx4
Allelic
Composition		 Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGK@yK1)9.2CJkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGK@yK1)9.2CJkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased immature B cell number ( J:137738 )
• immature B cells in circulation are at 70% of wild-type levels
decreased mature B cell number ( J:137738 )
• mature B cells are at 50-60% of wild-type levels in the spleen
• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-Ctm1Jkbv homozygotes
• mature B cells expressing human kappa light chain are found in similar numbers as B cells expressing murine lambda light chain
increased pre-B cell number ( J:137738 )
• there is a 2-fold accumulation of small pre- B cells in the bone marrow
abnormal immunoglobulin level ( J:137738 )
• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-Ctm1Jkbv homozygotes
• the concentration of antibodies containing human kappa light chain is 250 micrograms per ml in sera

hematopoietic system
decreased immature B cell number ( J:137738 )
• immature B cells in circulation are at 70% of wild-type levels
decreased mature B cell number ( J:137738 )
• mature B cells are at 50-60% of wild-type levels in the spleen
• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-Ctm1Jkbv homozygotes
• mature B cells expressing human kappa light chain are found in similar numbers as B cells expressing murine lambda light chain
increased pre-B cell number ( J:137738 )
• there is a 2-fold accumulation of small pre- B cells in the bone marrow
abnormal immunoglobulin level ( J:137738 )
• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-Ctm1Jkbv homozygotes
• the concentration of antibodies containing human kappa light chain is 250 micrograms per ml in sera




Genotype
MGI:3802931
cx5
Allelic
Composition		 Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGK@yK1)9.2CJkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:137738 )
• B cells exclusively express lambda light chain and not kappa light chain
decreased immature B cell number ( J:137738 )
• immature B cells in circulation are at 70% of wild-type levels
decreased mature B cell number ( J:137738 )
• mature B cells are at 50-60% of wild-type levels in the spleen
• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-Ctm1Jkbv homozygotes
• mature B cells expressing human kappa light chain are twice as common as B cells expressing murine lambda light chain
increased pre-B cell number ( J:137738 )
• there is a 2-fold accumulation of small pre- B cells in the bone marrow
abnormal immunoglobulin level ( J:137738 )
• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-Ctm1Jkbv homozygotes
• the concentration of antibodies containing human kappa light chain is 720 micrograms per ml in sera

hematopoietic system
abnormal B cell differentiation ( J:137738 )
• B cells exclusively express lambda light chain and not kappa light chain
decreased immature B cell number ( J:137738 )
• immature B cells in circulation are at 70% of wild-type levels
decreased mature B cell number ( J:137738 )
• mature B cells are at 50-60% of wild-type levels in the spleen
• mature B cells in the blood and lymph nodes are 20-25% higher than in Igk-Ctm1Jkbv homozygotes
• mature B cells expressing human kappa light chain are twice as common as B cells expressing murine lambda light chain
increased pre-B cell number ( J:137738 )
• there is a 2-fold accumulation of small pre- B cells in the bone marrow
abnormal immunoglobulin level ( J:137738 )
• the concentration of antibodies containing murine lambda light chain are reduced to levels of 300 micrograms per ml compared to 580 micrograms per ml in Igk-Ctm1Jkbv homozygotes
• the concentration of antibodies containing human kappa light chain is 720 micrograms per ml in sera




Genotype
MGI:3802934
cx6
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH1)2BJkbv/0
Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH1)2BJkbv mutation (0 available)
Tg(IGK@yK1)9.2CJkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:137738 )
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
decreased immature B cell number ( J:137738 )
• B cells newly emerging from the bone marrow are at levels that are 10% of that in wild-type mice
increased late pro-B cell number ( J:137738 )
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
decreased mature B cell number ( J:137738 )
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
decreased pre-B cell number ( J:137738 )
• pre-B cells numbers in the bone marrow are 30% of wild-type

hematopoietic system
abnormal B cell differentiation ( J:137738 )
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
decreased immature B cell number ( J:137738 )
• B cells newly emerging from the bone marrow are at levels that are 10% of that in wild-type mice
increased late pro-B cell number ( J:137738 )
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
decreased mature B cell number ( J:137738 )
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
decreased pre-B cell number ( J:137738 )
• pre-B cells numbers in the bone marrow are 30% of wild-type




Genotype
MGI:3802935
cx7
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH1)2BJkbv/Tg(IGH@yH1)2BJkbv
Tg(IGK@yK1)9.2CJkbv/Tg(IGK@yK1)9.2CJkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH1)2BJkbv mutation (0 available)
Tg(IGK@yK1)9.2CJkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:137738 )
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
decreased immature B cell number ( J:137738 )
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
increased late pro-B cell number ( J:137738 )
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
decreased mature B cell number ( J:137738 )
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
decreased pre-B cell number ( J:137738 )
• pre-B cells numbers in the bone marrow are 70% of wild-type
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 150 and 120 micrograms per ml, respectively

hematopoietic system
abnormal B cell differentiation ( J:137738 )
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
decreased immature B cell number ( J:137738 )
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
increased late pro-B cell number ( J:137738 )
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
decreased mature B cell number ( J:137738 )
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
decreased pre-B cell number ( J:137738 )
• pre-B cells numbers in the bone marrow are 70% of wild-type
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 150 and 120 micrograms per ml, respectively




Genotype
MGI:3802678
tg8
Allelic
Composition		 Tg(IGK@yK1)9.2CJkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell morphology ( J:23827 )
• 9.3% of B cells express human kappa light chain
• 80% of the B cells that express human kappa light chain do not express endogenous murine light chains
abnormal immunoglobulin light chain V-J recombination ( J:23827 )
• the V and J segments of the human transgene undergo recombination within B cells
abnormal immunoglobulin level ( J:23827 )
• antibodies containing the human kappa light chain is found in the sera at 17.7 micrograms per ml

hematopoietic system
abnormal B cell morphology ( J:23827 )
• 9.3% of B cells express human kappa light chain
• 80% of the B cells that express human kappa light chain do not express endogenous murine light chains
abnormal immunoglobulin light chain V-J recombination ( J:23827 )
• the V and J segments of the human transgene undergo recombination within B cells
abnormal immunoglobulin level ( J:23827 )
• antibodies containing the human kappa light chain is found in the sera at 17.7 micrograms per ml




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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory