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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(IGH@yH2)J9.2Jkbv
transgene insertion J9.2, Aya Jakobovits
MGI:3802713
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0 		involves: 129P2/OlaHsd MGI:3802926
cx2
 		Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv 		involves: 129P2/OlaHsd MGI:3802937
cx3
 		Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Tg(IGK@yK2)J23.1Jkbv/0 		involves: 129P2/OlaHsd * C57BL/6J MGI:3802734


Genotype
MGI:3802926
cx1
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Tg(IGH@yH2)J9.2Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:137738 )
N
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
decreased mature B cell number ( J:137738 )
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu or gamma2 heavy chains are found in the sera at levels of 200 micrograms per ml

hematopoietic system
decreased mature B cell number ( J:137738 )
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu or gamma2 heavy chains are found in the sera at levels of 200 micrograms per ml




Genotype
MGI:3802937
cx2
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH2)J9.2Jkbv mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:137738 )
N
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
decreased immature B cell number ( J:137738 )
• the levels of immature B cells found in circulation are 70% that found in wild-type mice
decreased mature B cell number ( J:137738 )
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively

hematopoietic system
decreased immature B cell number ( J:137738 )
• the levels of immature B cells found in circulation are 70% that found in wild-type mice
decreased mature B cell number ( J:137738 )
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively




Genotype
MGI:3802734
cx3
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH2)J9.2Jkbv mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell morphology ( J:70620 )
• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
abnormal immunoglobulin heavy chain V(D)J recombination ( J:70620 )
• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
abnormal immunoglobulin light chain V-J recombination ( J:70620 )
• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
increased mature B cell number ( J:70620 )
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
abnormal immunoglobulin level ( J:70620 )
• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen

hematopoietic system
abnormal B cell morphology ( J:70620 )
• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
abnormal immunoglobulin heavy chain V(D)J recombination ( J:70620 )
• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
abnormal immunoglobulin light chain V-J recombination ( J:70620 )
• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
increased mature B cell number ( J:70620 )
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
abnormal immunoglobulin level ( J:70620 )
• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen




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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory