About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(IGK@yK2)J23.1Jkbv
transgene insertion J23.1, Aya Jakobovits
MGI:3802714
Summary 9 genotypes


Genotype
MGI:3802932
cx1
Allelic
Composition		 Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:137738 )
N
• the presence of the transgene prevents the partial arrest of B cell development that occurs in Igk-Ctm1Jkbv homozygotes
• normal numbers of B cells are found at all developmental stages including immature and mature stages
abnormal B cell morphology ( J:137738 )
• 75% of mature B cells express human kappa light chain
abnormal immunoglobulin level ( J:137738 )
• levels of antibody containing human kappa light chain are 1,400 micrograms per ml while levels of antibody containing murine lambda light chain are 100 micrograms per ml

hematopoietic system
abnormal B cell morphology ( J:137738 )
• 75% of mature B cells express human kappa light chain
abnormal immunoglobulin level ( J:137738 )
• levels of antibody containing human kappa light chain are 1,400 micrograms per ml while levels of antibody containing murine lambda light chain are 100 micrograms per ml




Genotype
MGI:3802933
cx2
Allelic
Composition		 Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:137738 )
N
• the presence of the transgene prevents the partial arrest of B cell development that occurs in Igk-Ctm1Jkbv homozygotes
• normal numbers of B cells are found at all developmental stages including immature and mature stages
abnormal B cell morphology ( J:137738 )
• over 90% of mature B cells express human kappa light chain

hematopoietic system
abnormal B cell morphology ( J:137738 )
• over 90% of mature B cells express human kappa light chain




Genotype
MGI:3802936
cx3
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH1)2BJkbv/Tg(IGH@yH1)2BJkbv
Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH1)2BJkbv mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:137738 )
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
decreased immature B cell number ( J:137738 )
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
increased late pro-B cell number ( J:137738 )
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
decreased mature B cell number ( J:137738 )
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
decreased pre-B cell number ( J:137738 )
• pre-B cells numbers in the bone marrow are 70% of wild-type

hematopoietic system
abnormal B cell differentiation ( J:137738 )
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
decreased immature B cell number ( J:137738 )
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
increased late pro-B cell number ( J:137738 )
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
decreased mature B cell number ( J:137738 )
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
decreased pre-B cell number ( J:137738 )
• pre-B cells numbers in the bone marrow are 70% of wild-type




Genotype
MGI:3802937
cx4
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Tg(IGK@yK2)J23.1Jkbv/Tg(IGK@yK2)J23.1Jkbv
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH2)J9.2Jkbv mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:137738 )
N
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
decreased immature B cell number ( J:137738 )
• the levels of immature B cells found in circulation are 70% that found in wild-type mice
decreased mature B cell number ( J:137738 )
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively

hematopoietic system
decreased immature B cell number ( J:137738 )
• the levels of immature B cells found in circulation are 70% that found in wild-type mice
decreased mature B cell number ( J:137738 )
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
abnormal immunoglobulin level ( J:137738 )
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively




Genotype
MGI:3810268
cx5
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2BM)7Amgn/0
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH2BM)7Amgn mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased B cell number ( J:139792 )
• mice have 60-80% the number of B cells as wild-type mice
abnormal immunoglobulin level ( J:139792 )
• unimmunized mice produce significant levels of human IgM and IgG1
• IgM levels in sera ranged from 110-140 micrograms/ml
• IgG1 levels in sera ranged from 172-298 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences

hematopoietic system
decreased B cell number ( J:139792 )
• mice have 60-80% the number of B cells as wild-type mice
abnormal immunoglobulin level ( J:139792 )
• unimmunized mice produce significant levels of human IgM and IgG1
• IgM levels in sera ranged from 110-140 micrograms/ml
• IgG1 levels in sera ranged from 172-298 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences




Genotype
MGI:3810269
cx6
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2CM)2Amgn/0
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH2CM)2Amgn mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased B cell number ( J:139792 )
• mice have 80-100% the number of B cells as wild-type mice
abnormal immunoglobulin level ( J:139792 )
• unimmunized mice produce significant levels of human IgM and IgG2
• IgM levels in sera ranged from 27.3-109.4 micrograms/ml
• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences

hematopoietic system
decreased B cell number ( J:139792 )
• mice have 80-100% the number of B cells as wild-type mice
abnormal immunoglobulin level ( J:139792 )
• unimmunized mice produce significant levels of human IgM and IgG2
• IgM levels in sera ranged from 27.3-109.4 micrograms/ml
• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences




Genotype
MGI:3810270
cx7
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yHG1/2)1Amgn/0
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yHG1/2)1Amgn mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal immunoglobulin level ( J:139792 )
• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences

hematopoietic system
abnormal immunoglobulin level ( J:139792 )
• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences




Genotype
MGI:3810271
cx8
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yHG4)1Amgn/0
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yHG4)1Amgn mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal immunoglobulin level ( J:139792 )
• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences

hematopoietic system
abnormal immunoglobulin level ( J:139792 )
• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences




Genotype
MGI:3802734
cx9
Allelic
Composition		 Igh-Jtm1Jkbv/Igh-Jtm1Jkbv
Igkctm1Jkbv/Igkctm1Jkbv
Tg(IGH@yH2)J9.2Jkbv/0
Tg(IGK@yK2)J23.1Jkbv/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation (0 available); any Igh-J mutation (13 available)
Igkctm1Jkbv mutation (0 available); any Igkc mutation (9 available)
Tg(IGH@yH2)J9.2Jkbv mutation (0 available)
Tg(IGK@yK2)J23.1Jkbv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell morphology ( J:70620 )
• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
abnormal immunoglobulin heavy chain V(D)J recombination ( J:70620 )
• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
abnormal immunoglobulin light chain V-J recombination ( J:70620 )
• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
increased mature B cell number ( J:70620 )
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
abnormal immunoglobulin level ( J:70620 )
• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen

hematopoietic system
abnormal B cell morphology ( J:70620 )
• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
abnormal immunoglobulin heavy chain V(D)J recombination ( J:70620 )
• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
abnormal immunoglobulin light chain V-J recombination ( J:70620 )
• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
increased mature B cell number ( J:70620 )
• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
abnormal immunoglobulin level ( J:70620 )
• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory