All Phenotypes Hand2<tm1.1Majh> MGI Mouse

embryonic lethality during organogenesis, complete penetrance ( J:137726 )

• embryos die around E12

• treatment of dams with a mixture of catecholamine intermediates allows mice to survive to birth

N	• despite loss of Hand2 expression in neural crest cells, neural crest migration is normal

abnormal neuron differentiation ( J:137726 )

• significant reduction in the proliferation of neuronal precursor cells and neuroblasts by E12

abnormal enteric nervous system morphology ( J:138032 )

• patterning defects in the stomach suggest decrease in fiber density and relative disorganization of the plexus

abnormal enteric ganglia morphology ( J:138032 )

• abnormal patterning of ganglia and decrease in ganglia depth in the stomach at E14

• at E16 decrease in fiber and ganglia density at the oral end of the stomach

• density increases from the oral to the anal end but remains lower than in stomachs of control embryos

abnormal enteric neuron morphology ( J:138032 )

• marked increase in cell size and decrease in cell density in the stomach at E14

• at E16 decrease in fiber and ganglia density at the oral end of the stomach

• density increases from the oral to the anal end but remains lower than in stomachs of control embryos

abnormal sympathetic ganglion morphology ( J:137726 )

• at E12 there is a reduction in the number of cells expressing either a pan-neuronal marker (Hu) and catecholaminergic marker (TH) as well as a decrease in the proportion of cells expressing both markers

• the proportion of neurons and expression of TH continues to decrease with age

• at P0 only a few scattered cells remain that express TH and the sympathetic chain is absent

abnormal heart development ( J:137726 )