Phenotypes associated with this allele

• Allele Symbol: Tg(Fcer2a-cre)5Mbu
• Allele Name: transgene insertion 5, Meinrad Busslinger
• Allele ID: MGI:3803652
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Atmin^tm1.1Jhh/Atmin^tm1.1Jhh Ighm^tm1(Bcl6)Rdf/Ighm^+ Tg(Fcer2a-cre)5Mbu/0	B6.Cg-Atmin^tm1.1Jhh Ighm^tm1(Bcl6)Rdf Tg(Fcer2a-cre)5Mbu	MGI:5755471
cn2	Atmin^tm1.1Jhh/Atmin^tm1.1Jhh Tg(Fcer2a-cre)5Mbu/0	B6.Cg-Atmin^tm1.1Jhh Tg(Fcer2a-cre)5Mbu	MGI:5755467
cn3	Eif4a1^tm1.1Bea/Eif4a1^tm1.1Bea Tg(Fcer2a-cre)5Mbu/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:7646992
cn4	Hdac1^tm1.1Pmt/Hdac1^tm1.1Pmt Hdac2^tm1.1Pmt/Hdac2^tm1.1Pmt Tg(Fcer2a-cre)5Mbu/0	involves: 129P2/OlaHsd * C57BL/6 * CBA * FVB/N	MGI:4440610
cn5	Tcf3^tm1Mbu/Tcf3^tm1.2Mbu Tg(Fcer2a-cre)5Mbu/0	involves: 129P2/OlaHsd * C57BL/6 * CBA * SJL	MGI:3804199
cn6	Nfatc1^tm1Rao/Nfatc1^tm1Rao Tg(Fcer2a-cre)5Mbu/0	involves: C57BL/6 * CBA	MGI:5295254
cn7	Rtcb^tm1c(KOMP)Wtsi/Rtcb^tm1c(KOMP)Wtsi Tg(Fcer2a-cre)5Mbu/0	involves: C57BL/6 * CBA * SJL	MGI:5688397

Genotype
MGI:5755471

cn1

• Allelic Composition: Atmin^tm1.1Jhh/Atmin^tm1.1Jhh; Ighm^tm1(Bcl6)Rdf/Ighm⁺; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: B6.Cg-Atmin^tm1.1Jhh Ighm^tm1(Bcl6)Rdf Tg(Fcer2a-cre)5Mbu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

abnormal tumor susceptibility ( J:230105 )

• mice exhibit similar tumorigenesis as Atmin^tm1.1Jhh/Atmin^tm1.1Jhh Tg(Fcer2a-cre)5Mbu mice

Genotype
MGI:5755467

cn2

• Allelic Composition: Atmin^tm1.1Jhh/Atmin^tm1.1Jhh; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: B6.Cg-Atmin^tm1.1Jhh Tg(Fcer2a-cre)5Mbu

hematopoietic system

hematopoietic system phenotype ( J:230105 )

N • mice exhibit normal B cell numbers in the spleen and antigen-specific IgG1-switched antibodies

neoplasm

neoplasm ( J:230105 )

N • mice repeatedly immunized with sheep red blood cells do not exhibit an increase in tumor-related death

Genotype
MGI:7646992

cn3

• Allelic Composition: Eif4a1^tm1.1Bea/Eif4a1^tm1.1Bea; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

hematopoietic system

hematopoietic system phenotype ( J:344730 )

N • normal numbers of splenic immature B cells

decreased B cell proliferation ( J:344730 )

• in a mixed bone marrow competitive chimera the number of Fo and MZ cells derived from null mice are reduced by 47% and 59%, respectively

• in culture the proliferation index is reduced to 1 compared to 3 in control cells

decreased germinal center B cell number ( J:344730 )

• 7 days after immunization with 4-hydroxy-3-nitrophenyl-acetyl conjugated to keyhole limpet hemocyanin germinal center B cells are almost completely absent with a 10-fold reduction compared to similarly treated control mice

• light zone and dark zone germinal center B cells are reduced by 9-fold and 15-fold, respectively

• reduction in B cells corresponds to a greater than 100-fold reduction in NP-specific IgG1 antibody secreting cells in the spleen

• remaining B cells have only a 40% reduction in EIF4A1

decreased IgG1 level ( J:344730 )

• decrease in both low- and high-affinity IgG1 antibodies following immunization

cellular

decreased B cell proliferation ( J:344730 )

• in a mixed bone marrow competitive chimera the number of Fo and MZ cells derived from null mice are reduced by 47% and 59%, respectively

• in culture the proliferation index is reduced to 1 compared to 3 in control cells

immune system

decreased B cell proliferation ( J:344730 )

• in a mixed bone marrow competitive chimera the number of Fo and MZ cells derived from null mice are reduced by 47% and 59%, respectively

• in culture the proliferation index is reduced to 1 compared to 3 in control cells

decreased germinal center B cell number ( J:344730 )

• 7 days after immunization with 4-hydroxy-3-nitrophenyl-acetyl conjugated to keyhole limpet hemocyanin germinal center B cells are almost completely absent with a 10-fold reduction compared to similarly treated control mice

• light zone and dark zone germinal center B cells are reduced by 9-fold and 15-fold, respectively

• reduction in B cells corresponds to a greater than 100-fold reduction in NP-specific IgG1 antibody secreting cells in the spleen

• remaining B cells have only a 40% reduction in EIF4A1

decreased IgG1 level ( J:344730 )

• decrease in both low- and high-affinity IgG1 antibodies following immunization

Genotype
MGI:4440610

cn4

• Allelic Composition: Hdac1^tm1.1Pmt/Hdac1^tm1.1Pmt; Hdac2^tm1.1Pmt/Hdac2^tm1.1Pmt; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA * FVB/N

immune system

increased B cell apoptosis ( J:157903 )

• massive induction of apoptosis is detected when cells are cultured with LPS and IL4 or anti-IgM Fab

decreased B cell proliferation ( J:157903 )

• fail to proliferate in response to LPS and IL4 or anti-IgM Fab fragment stimulation

hematopoietic system

increased B cell apoptosis ( J:157903 )

• massive induction of apoptosis is detected when cells are cultured with LPS and IL4 or anti-IgM Fab

decreased B cell proliferation ( J:157903 )

• fail to proliferate in response to LPS and IL4 or anti-IgM Fab fragment stimulation

cellular

increased B cell apoptosis ( J:157903 )

• massive induction of apoptosis is detected when cells are cultured with LPS and IL4 or anti-IgM Fab

decreased B cell proliferation ( J:157903 )

• fail to proliferate in response to LPS and IL4 or anti-IgM Fab fragment stimulation

Genotype
MGI:3804199

cn5

• Allelic Composition: Tcf3^tm1Mbu/Tcf3^tm1.2Mbu; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA * SJL

immune system

immune system phenotype ( J:137719 )

N • despite reduced B cell numbers, class switching, NP-specific production of IgG1, IgG2a and IgG3, B cell proliferation, memory B cells and plasma B cells following stimulation are normal following immunization

increased marginal zone B cell number ( J:137719 )

• slight

decreased B cell number ( J:137719 )

• splenic B cell numbers are minimally reduced compared to in wild-type mice

abnormal germinal center B cell morphology ( J:137719 )

• 8 days following immunization, PNA+ germinal center number and size are reduced compared to in wild-type or heterozygous mice

• however, stimulated germinal B cell proliferation is normal

decreased germinal center B cell number ( J:137719 )

• at 4 and 8 days after immunization germinal center B cell numbers are 3- to 5-fold lower tan in wild-type or heterozygous mice

hematopoietic system

increased marginal zone B cell number ( J:137719 )

• slight

decreased B cell number ( J:137719 )

• splenic B cell numbers are minimally reduced compared to in wild-type mice

decreased germinal center B cell number ( J:137719 )

• at 4 and 8 days after immunization germinal center B cell numbers are 3- to 5-fold lower tan in wild-type or heterozygous mice

abnormal germinal center B cell morphology ( J:137719 )

• 8 days following immunization, PNA+ germinal center number and size are reduced compared to in wild-type or heterozygous mice

• however, stimulated germinal B cell proliferation is normal

Genotype
MGI:5295254

cn6

• Allelic Composition: Nfatc1^tm1Rao/Nfatc1^tm1Rao; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

abnormal B cell physiology ( J:177319 )

• stimulated B cells exhibit reduced ability to induced T cell proliferation

decreased B cell proliferation ( J:177319 )

• alpha-IgM-mediated

• however, LPS-stimulated B cell proliferation is normal

abnormal class switch recombination ( J:177319 )

• to IgG3 induced by T cell-independent type II antigens

decreased IgG2a level ( J:177319 )

• after immunization with NP-KLH

decreased IgG3 level ( J:177319 )

• 10-fold after immunization with NP-Ficoll

decreased IgM level ( J:177319 )

• after immunization with NP-Ficoll

increased IgM level ( J:177319 )

• in unstimulated B cells

increased interleukin-10 secretion ( J:177319 )

• from LPS-stimulated B cells

decreased interleukin-2 secretion ( J:177319 )

• from stimulated B cells

hematopoietic system

abnormal B cell physiology ( J:177319 )

• stimulated B cells exhibit reduced ability to induced T cell proliferation

decreased B cell proliferation ( J:177319 )

• alpha-IgM-mediated

• however, LPS-stimulated B cell proliferation is normal

abnormal class switch recombination ( J:177319 )

• to IgG3 induced by T cell-independent type II antigens

decreased IgG2a level ( J:177319 )

• after immunization with NP-KLH

decreased IgG3 level ( J:177319 )

• 10-fold after immunization with NP-Ficoll

decreased IgM level ( J:177319 )

• after immunization with NP-Ficoll

increased IgM level ( J:177319 )

• in unstimulated B cells

cellular

decreased B cell proliferation ( J:177319 )

• alpha-IgM-mediated

• however, LPS-stimulated B cell proliferation is normal

Genotype
MGI:5688397

cn7

• Allelic Composition: Rtcb^{tm1c(KOMP)Wtsi}/Rtcb^{tm1c(KOMP)Wtsi}; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: involves: C57BL/6 * CBA * SJL

immune system

abnormal B cell number ( J:216740 )

• slight decrease in the percentage of CD138+ CD22- plasmablasts in cells stimulated with LPS in vitro

abnormal plasma cell morphology ( J:216740 )

• plasmablasts have disorganized and less dense ER structures

abnormal B cell physiology ( J:216740 )

• impaired ability of plasma cells to secrete immunoglobins

decreased IgM level ( J:216740 )

• in culture supernatants of pre-plasmablasts and plasmablasts

• however, the number of plasmablasts secreting IgM is similar to control cultures

hematopoietic system

abnormal B cell number ( J:216740 )

• slight decrease in the percentage of CD138+ CD22- plasmablasts in cells stimulated with LPS in vitro

abnormal plasma cell morphology ( J:216740 )

• plasmablasts have disorganized and less dense ER structures

abnormal B cell physiology ( J:216740 )

• impaired ability of plasma cells to secrete immunoglobins

decreased IgM level ( J:216740 )

• in culture supernatants of pre-plasmablasts and plasmablasts

• however, the number of plasmablasts secreting IgM is similar to control cultures