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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Lck-Akt1*)M-1Pnt
transgene insertion M-1, Philip N Tsichlis
MGI:3803798
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Rag2tm1Fwa/Rag2tm1Fwa
Tg(Lck-Akt1*)M-1Pnt/0 		involves: 129S/SvEv * C3H/He * C57BL/6 MGI:3803973
tg2
 		Tg(Lck-Akt1*)M-1Pnt/0 involves: C3H/He * C57BL/6 MGI:3803812


Genotype
MGI:3803973
cx1
Allelic
Composition		 Rag2tm1Fwa/Rag2tm1Fwa
Tg(Lck-Akt1*)M-1Pnt/0
Genetic
Background		 involves: 129S/SvEv * C3H/He * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(Lck-Akt1*)M-1Pnt mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased double-positive T cell number ( J:135903 )
• 96% of thymocytes are double positive
• the presence of the transgene rescues the block of T cell development that occurs at DN3 in Rag2tm1Fwa homozygotes
absent CD4-positive, alpha-beta T cells ( J:135903 )
• despite the presence of double positive thymocytes, single positive T cells do not develop
absent CD8-positive, alpha-beta T cells ( J:135903 )
• despite the presence of double positive thymocytes, single positive T cells do not develop

hematopoietic system
increased double-positive T cell number ( J:135903 )
• 96% of thymocytes are double positive
• the presence of the transgene rescues the block of T cell development that occurs at DN3 in Rag2tm1Fwa homozygotes
absent CD4-positive, alpha-beta T cells ( J:135903 )
• despite the presence of double positive thymocytes, single positive T cells do not develop
absent CD8-positive, alpha-beta T cells ( J:135903 )
• despite the presence of double positive thymocytes, single positive T cells do not develop




Genotype
MGI:3803812
tg2
Allelic
Composition		 Tg(Lck-Akt1*)M-1Pnt/0
Genetic
Background		 involves: C3H/He * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
decreased survivor rate ( J:73487 )
• about half of the mice are dead at 90 days of age from thymic lymphomas
• less than 20% of the mice live past 240 days

neoplasm
increased T cell derived lymphoma incidence ( J:73487 )
• most mice die of thymic lymphomas
• lymphomas contain clonal rearrangements in the T cell receptor genes
• all tumors are lymphoblastic lymphomas with variable expression of the CD4 and CD8 genes

immune system
decreased thymocyte number ( J:73487 )
• number of thymocytes is decreased by more than 20% compared to wild-type mice despite the thymus having similar weight as in wild-type
increased T cell derived lymphoma incidence ( J:73487 )
• most mice die of thymic lymphomas
• lymphomas contain clonal rearrangements in the T cell receptor genes
• all tumors are lymphoblastic lymphomas with variable expression of the CD4 and CD8 genes
enlarged spleen ( J:73487 )
• is often observed in necropsy due to lymphomas
increased double-negative T cell number ( J:135903 )
• there is a five-fold increase in the percentage of double negative thymocytes found in the thymus
• double negative thymocytes progress rapidly through the DN3 to DN4 stage
increased single-positive T cell number ( J:135903 )
• the percentage of single positive thymocytes is increased in these mice

cellular
abnormal cell cycle checkpoint function ( J:73487 )
• thymocyte cell size is significantly increased in these mice
• thymocytes express high levels of cyclin D2, D3,and E and promote progression through G1

hematopoietic system
decreased thymocyte number ( J:73487 )
• number of thymocytes is decreased by more than 20% compared to wild-type mice despite the thymus having similar weight as in wild-type
increased T cell derived lymphoma incidence ( J:73487 )
• most mice die of thymic lymphomas
• lymphomas contain clonal rearrangements in the T cell receptor genes
• all tumors are lymphoblastic lymphomas with variable expression of the CD4 and CD8 genes
enlarged spleen ( J:73487 )
• is often observed in necropsy due to lymphomas
increased double-negative T cell number ( J:135903 )
• there is a five-fold increase in the percentage of double negative thymocytes found in the thymus
• double negative thymocytes progress rapidly through the DN3 to DN4 stage
increased single-positive T cell number ( J:135903 )
• the percentage of single positive thymocytes is increased in these mice

endocrine/exocrine glands
decreased thymocyte number ( J:73487 )
• number of thymocytes is decreased by more than 20% compared to wild-type mice despite the thymus having similar weight as in wild-type
increased T cell derived lymphoma incidence ( J:73487 )
• most mice die of thymic lymphomas
• lymphomas contain clonal rearrangements in the T cell receptor genes
• all tumors are lymphoblastic lymphomas with variable expression of the CD4 and CD8 genes

growth/size/body
enlarged spleen ( J:73487 )
• is often observed in necropsy due to lymphomas




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory