Analysis Tools
hematopoietic system
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• after BCR crosslinking, B cells isolated from mutant mice show reduced proliferative capacity compared to wild-type cells
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• overall spleen cell number is greater than in wild-type
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• transplantation of mutant bone marrow cells into irradiated mice recapitulates the MZ and FO B cell defects observed in Fli1-null mice, indicating that defects are B cell-autonomous
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• in vitro, IgG1 class switch of B cells is reduced compared to wild-type B cells stimulated with LPS, LPS and Il-4, or LPS and Ifng; percentage of IgG1-expressing B cells is significantly lower than from wild-type
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• absolute number is 10-fold higher than in controls; percentages of T1 B and marginal zone precursor B cells in spleen are significantly increased
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• percentage and total number of marginal zone (MZ) B cells are significantly increased compared to wild-type controls
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• percentage and total number of follicular zone (FO) B cells are significantly decreased compared to wild-type controls
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• percentage of splenic B cells is significantly decreased compared to wild-type controls, but due to increase in total cellularity, splenic B cell number is slightly greater than wild-type
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• decreased >50% compared with controls
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• decreased >50% compared with controls
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immune system
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• after BCR crosslinking, B cells isolated from mutant mice show reduced proliferative capacity compared to wild-type cells
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• overall spleen cell number is greater than in wild-type
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• transplantation of mutant bone marrow cells into irradiated mice recapitulates the MZ and FO B cell defects observed in Fli1-null mice, indicating that defects are B cell-autonomous
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• in vitro, IgG1 class switch of B cells is reduced compared to wild-type B cells stimulated with LPS, LPS and Il-4, or LPS and Ifng; percentage of IgG1-expressing B cells is significantly lower than from wild-type
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• absolute number is 10-fold higher than in controls; percentages of T1 B and marginal zone precursor B cells in spleen are significantly increased
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• percentage and total number of marginal zone (MZ) B cells are significantly increased compared to wild-type controls
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• percentage and total number of follicular zone (FO) B cells are significantly decreased compared to wild-type controls
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• percentage of splenic B cells is significantly decreased compared to wild-type controls, but due to increase in total cellularity, splenic B cell number is slightly greater than wild-type
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• production of IgG1 antibodies against a T cell-dependent antigen (TNP-KLH) after immunization is reduced relative to wild-type controls
• mice produce higher levels of IgM antibodies against a T cell-independent antigen (TNP-Ficoll) after immunization compared to controls; mice also produce higher levels of IgG3 antibodies against both T cell-dependent and-independent antigens
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• decreased >50% compared with controls
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• decreased >50% compared with controls
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cellular
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• after BCR crosslinking, B cells isolated from mutant mice show reduced proliferative capacity compared to wild-type cells
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growth/size/body
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• overall spleen cell number is greater than in wild-type
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